Strain Improvement Via Random Mutation For Glucoamylase Production By Aspergillus Niger Van Tieghem Through Solid State Fermentation
Glucoamylase enzyme is widely utilized for conversion of starch into glucose during saccharification process. Aspergillus niger van Tieghem is commonly used for glucoamylase production in submerged cultivation system (SmF) and solid state fermentation (SSF). This research was carried out to enhan...
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| Format: | Thesis |
| Language: | English |
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2012
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| Online Access: | http://eprints.usm.my/59458/1/24%20Pages%20from%2000001760344.pdf |
| _version_ | 1825908091134148608 |
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| author | Murugaiah, Hemalatha |
| author_facet | Murugaiah, Hemalatha |
| author_sort | Murugaiah, Hemalatha |
| collection | USM |
| description | Glucoamylase enzyme is widely utilized for conversion of starch into glucose during
saccharification process. Aspergillus niger van Tieghem is commonly used for
glucoamylase production in submerged cultivation system (SmF) and solid state
fermentation (SSF). This research was carried out to enhance glucoamylase
production by mutating A. niger van Tieghem using chemical mutagen, ethyl
methane sulphonate (EMS) via random mutation. Mutant ANC9 was identified as a
potential mutant which produced, 1.9 and 1.6 fold higher glucoamylase activity
towards cooked starch and raw starch respectively in SmF using cooked starch as
substrate. Glucoamylase activity towards raw starch and cooked starch in SSF by
mutant ANC9 were 44.81± 1.2 Ulg and 101.50 ±2.2 Ulg respectively compared to
wild strain which was only able to produce 34.4±O.5 Ulg towards cooked starch and
79.1±2.2 Ulg towards raw starch. |
| first_indexed | 2024-03-06T16:13:49Z |
| format | Thesis |
| id | usm.eprints-59458 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T16:13:49Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | usm.eprints-594582023-10-05T04:16:10Z http://eprints.usm.my/59458/ Strain Improvement Via Random Mutation For Glucoamylase Production By Aspergillus Niger Van Tieghem Through Solid State Fermentation Murugaiah, Hemalatha QH1-278.5 Natural history (General) Glucoamylase enzyme is widely utilized for conversion of starch into glucose during saccharification process. Aspergillus niger van Tieghem is commonly used for glucoamylase production in submerged cultivation system (SmF) and solid state fermentation (SSF). This research was carried out to enhance glucoamylase production by mutating A. niger van Tieghem using chemical mutagen, ethyl methane sulphonate (EMS) via random mutation. Mutant ANC9 was identified as a potential mutant which produced, 1.9 and 1.6 fold higher glucoamylase activity towards cooked starch and raw starch respectively in SmF using cooked starch as substrate. Glucoamylase activity towards raw starch and cooked starch in SSF by mutant ANC9 were 44.81± 1.2 Ulg and 101.50 ±2.2 Ulg respectively compared to wild strain which was only able to produce 34.4±O.5 Ulg towards cooked starch and 79.1±2.2 Ulg towards raw starch. 2012-07 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/59458/1/24%20Pages%20from%2000001760344.pdf Murugaiah, Hemalatha (2012) Strain Improvement Via Random Mutation For Glucoamylase Production By Aspergillus Niger Van Tieghem Through Solid State Fermentation. Masters thesis, Perpustakaan Hamzah Sendut. |
| spellingShingle | QH1-278.5 Natural history (General) Murugaiah, Hemalatha Strain Improvement Via Random Mutation For Glucoamylase Production By Aspergillus Niger Van Tieghem Through Solid State Fermentation |
| title | Strain Improvement Via Random Mutation For Glucoamylase Production By Aspergillus Niger Van Tieghem Through Solid State Fermentation |
| title_full | Strain Improvement Via Random Mutation For Glucoamylase Production By Aspergillus Niger Van Tieghem Through Solid State Fermentation |
| title_fullStr | Strain Improvement Via Random Mutation For Glucoamylase Production By Aspergillus Niger Van Tieghem Through Solid State Fermentation |
| title_full_unstemmed | Strain Improvement Via Random Mutation For Glucoamylase Production By Aspergillus Niger Van Tieghem Through Solid State Fermentation |
| title_short | Strain Improvement Via Random Mutation For Glucoamylase Production By Aspergillus Niger Van Tieghem Through Solid State Fermentation |
| title_sort | strain improvement via random mutation for glucoamylase production by aspergillus niger van tieghem through solid state fermentation |
| topic | QH1-278.5 Natural history (General) |
| url | http://eprints.usm.my/59458/1/24%20Pages%20from%2000001760344.pdf |
| work_keys_str_mv | AT murugaiahhemalatha strainimprovementviarandommutationforglucoamylaseproductionbyaspergillusnigervantieghemthroughsolidstatefermentation |