Identification of differentially expressed gene/s observed in stem cells derived extracted human tooth
The aim of this study was to identify the differentially expressed genes (DEGs) in SHED and DPSCs by GeneFishing™ DEG method using the arbitrary primer pairs provided. Two bands which were highly expressed in SHED and three bands in DPSCs were isolated purified and sent for sequencing. Sequencing...
| Main Author: | |
|---|---|
| Format: | Monograph |
| Language: | English |
| Published: |
Universiti Sains Malaysia
2012
|
| Subjects: | |
| Online Access: | http://eprints.usm.my/59592/1/DR%20KHAIRANI%20IDAH%20MOKHTAR%20-%20e.pdf |
| _version_ | 1825908117223768064 |
|---|---|
| author | Mokhtar, Khairaniidah |
| author_facet | Mokhtar, Khairaniidah |
| author_sort | Mokhtar, Khairaniidah |
| collection | USM |
| description | The aim of this study was to identify the differentially expressed genes (DEGs) in SHED and DPSCs by GeneFishing™ DEG method using the arbitrary primer pairs provided. Two bands
which were highly expressed in SHED and three bands in DPSCs were isolated purified and
sent for sequencing. Sequencing analysis revealed these to be TIMP Metallopeptidase
Inhibitor 1 (TIMP1}, (A09), and ribosomal protein s8, (RPS8), (A16) in SHED and collagen,
type I, alpha 1, (COL1AP), (A20), follistatin-like 1 (FSTL1), (A17), lectin, galactosidebinding,
soluble, 1, (LGALSP), (A16) in DPSCs. TIMP1 is involved in degradation of the
extracellular matrix, cell proliferation and anti-apoptotic function and RPS8 is involved as a
rate-limiting factor in translational regulation; COL1A1 is involved in the resistance and I elasticity of the tissues; FSTL1 is an autoantigen associated with rheumatoid arthritis;
LGALS1 is involved in cell growth, differentiation, adhesion, RNA processing, apoptosis, and
malignant transformation. The gene expression patterns of SHED and DPSCs might be useful
in determining the detailed functional roles of the relevant genes applicable to stem cell
therapies, which paves way to be used as I multipotent cell sources for genetic and tissue engineering technology. |
| first_indexed | 2024-03-06T16:14:12Z |
| format | Monograph |
| id | usm.eprints-59592 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T16:14:12Z |
| publishDate | 2012 |
| publisher | Universiti Sains Malaysia |
| record_format | dspace |
| spelling | usm.eprints-595922023-11-14T08:00:06Z http://eprints.usm.my/59592/ Identification of differentially expressed gene/s observed in stem cells derived extracted human tooth Mokhtar, Khairaniidah R735-854 Medical education. Medical schools. Research The aim of this study was to identify the differentially expressed genes (DEGs) in SHED and DPSCs by GeneFishing™ DEG method using the arbitrary primer pairs provided. Two bands which were highly expressed in SHED and three bands in DPSCs were isolated purified and sent for sequencing. Sequencing analysis revealed these to be TIMP Metallopeptidase Inhibitor 1 (TIMP1}, (A09), and ribosomal protein s8, (RPS8), (A16) in SHED and collagen, type I, alpha 1, (COL1AP), (A20), follistatin-like 1 (FSTL1), (A17), lectin, galactosidebinding, soluble, 1, (LGALSP), (A16) in DPSCs. TIMP1 is involved in degradation of the extracellular matrix, cell proliferation and anti-apoptotic function and RPS8 is involved as a rate-limiting factor in translational regulation; COL1A1 is involved in the resistance and I elasticity of the tissues; FSTL1 is an autoantigen associated with rheumatoid arthritis; LGALS1 is involved in cell growth, differentiation, adhesion, RNA processing, apoptosis, and malignant transformation. The gene expression patterns of SHED and DPSCs might be useful in determining the detailed functional roles of the relevant genes applicable to stem cell therapies, which paves way to be used as I multipotent cell sources for genetic and tissue engineering technology. Universiti Sains Malaysia 2012 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/59592/1/DR%20KHAIRANI%20IDAH%20MOKHTAR%20-%20e.pdf Mokhtar, Khairaniidah (2012) Identification of differentially expressed gene/s observed in stem cells derived extracted human tooth. Project Report. Universiti Sains Malaysia. (Submitted) |
| spellingShingle | R735-854 Medical education. Medical schools. Research Mokhtar, Khairaniidah Identification of differentially expressed gene/s observed in stem cells derived extracted human tooth |
| title | Identification of differentially expressed
gene/s observed in stem cells derived
extracted human tooth |
| title_full | Identification of differentially expressed
gene/s observed in stem cells derived
extracted human tooth |
| title_fullStr | Identification of differentially expressed
gene/s observed in stem cells derived
extracted human tooth |
| title_full_unstemmed | Identification of differentially expressed
gene/s observed in stem cells derived
extracted human tooth |
| title_short | Identification of differentially expressed
gene/s observed in stem cells derived
extracted human tooth |
| title_sort | identification of differentially expressed gene s observed in stem cells derived extracted human tooth |
| topic | R735-854 Medical education. Medical schools. Research |
| url | http://eprints.usm.my/59592/1/DR%20KHAIRANI%20IDAH%20MOKHTAR%20-%20e.pdf |
| work_keys_str_mv | AT mokhtarkhairaniidah identificationofdifferentiallyexpressedgenesobservedinstemcellsderivedextractedhumantooth |