Development Of An Efficient Protocol For The Production Of Phalaenopsis Hybrid Orchid Plantlets
As a monopodial plant, Phalaenopsis is traditionally propagated by off-shoot cutting or division. However, this resulted in a low multiplication rate and hinders the growth of the mother plant and therefore inefficient for large-scale production. Micropropagation has provided a valuable alternati...
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| Format: | Thesis |
| Language: | English |
| Published: |
2022
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| Online Access: | http://eprints.usm.my/59667/1/24%20Pages%20from%20ARULVILEE%20AP%20RAJASEGAR%20-%20TESIS.pdf |
| _version_ | 1825908131552559104 |
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| author | Rajasegar, Arulvilee |
| author_facet | Rajasegar, Arulvilee |
| author_sort | Rajasegar, Arulvilee |
| collection | USM |
| description | As a monopodial plant, Phalaenopsis is traditionally propagated by off-shoot
cutting or division. However, this resulted in a low multiplication rate and hinders
the growth of the mother plant and therefore inefficient for large-scale production.
Micropropagation has provided a valuable alternative method for the large-scale
production of identical orchid plantlets within a short period. The study aimed to
improve the proliferation of protocorm-like bodies (PLBs) of Phalaenopsis
Fuller’s Light × Phalaenopsis Purple Heart through the micropropagation method.
The thin cell layer culture (TCL) approach generated a 2.16 times higher neo-PLB
formation rate than the conventional half-PLB explants. The medium composition
of full MS medium supplemented with 3% sucrose, 2% banana extract, and 15%
coconutwater significantly produced the highest PLB fresh weight (2.499 g) after
4 weeks of culture. The Phalaenopsis PLBs further developed into shoot and root
using 1.0 mg/L BAP and NAA mg/L. The incorporation of 1.5 mg/L of glutamine
resulted shoot formation with 70% while 1.5 mg/L of arginine increased root
formation by 87%, wherein the cultures were differentiated into complete
plantlets. |
| first_indexed | 2024-03-06T16:14:24Z |
| format | Thesis |
| id | usm.eprints-59667 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T16:14:24Z |
| publishDate | 2022 |
| record_format | dspace |
| spelling | usm.eprints-596672023-12-13T01:28:02Z http://eprints.usm.my/59667/ Development Of An Efficient Protocol For The Production Of Phalaenopsis Hybrid Orchid Plantlets Rajasegar, Arulvilee QH1-278.5 Natural history (General) As a monopodial plant, Phalaenopsis is traditionally propagated by off-shoot cutting or division. However, this resulted in a low multiplication rate and hinders the growth of the mother plant and therefore inefficient for large-scale production. Micropropagation has provided a valuable alternative method for the large-scale production of identical orchid plantlets within a short period. The study aimed to improve the proliferation of protocorm-like bodies (PLBs) of Phalaenopsis Fuller’s Light × Phalaenopsis Purple Heart through the micropropagation method. The thin cell layer culture (TCL) approach generated a 2.16 times higher neo-PLB formation rate than the conventional half-PLB explants. The medium composition of full MS medium supplemented with 3% sucrose, 2% banana extract, and 15% coconutwater significantly produced the highest PLB fresh weight (2.499 g) after 4 weeks of culture. The Phalaenopsis PLBs further developed into shoot and root using 1.0 mg/L BAP and NAA mg/L. The incorporation of 1.5 mg/L of glutamine resulted shoot formation with 70% while 1.5 mg/L of arginine increased root formation by 87%, wherein the cultures were differentiated into complete plantlets. 2022-09 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/59667/1/24%20Pages%20from%20ARULVILEE%20AP%20RAJASEGAR%20-%20TESIS.pdf Rajasegar, Arulvilee (2022) Development Of An Efficient Protocol For The Production Of Phalaenopsis Hybrid Orchid Plantlets. PhD thesis, Perpustakaan Hamzah Sendut. |
| spellingShingle | QH1-278.5 Natural history (General) Rajasegar, Arulvilee Development Of An Efficient Protocol For The Production Of Phalaenopsis Hybrid Orchid Plantlets |
| title | Development Of An Efficient Protocol For The Production Of Phalaenopsis Hybrid Orchid Plantlets |
| title_full | Development Of An Efficient Protocol For The Production Of Phalaenopsis Hybrid Orchid Plantlets |
| title_fullStr | Development Of An Efficient Protocol For The Production Of Phalaenopsis Hybrid Orchid Plantlets |
| title_full_unstemmed | Development Of An Efficient Protocol For The Production Of Phalaenopsis Hybrid Orchid Plantlets |
| title_short | Development Of An Efficient Protocol For The Production Of Phalaenopsis Hybrid Orchid Plantlets |
| title_sort | development of an efficient protocol for the production of phalaenopsis hybrid orchid plantlets |
| topic | QH1-278.5 Natural history (General) |
| url | http://eprints.usm.my/59667/1/24%20Pages%20from%20ARULVILEE%20AP%20RAJASEGAR%20-%20TESIS.pdf |
| work_keys_str_mv | AT rajasegararulvilee developmentofanefficientprotocolfortheproductionofphalaenopsishybridorchidplantlets |