Transcription Start Site Mapping And Small Regulatory Rna Profiling Of Mycobacterium Tuberculosis
Mycobacterium tuberculosis is an actinobacterium that causes chronic infections. Its pathogenesis lies in the thick, lipid-rich cell envelope that facilitate persistence in hostile environments. A refined knowledge of regulatory networks within M. tuberculosis is important to understand its patho...
| मुख्य लेखक: | |
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| स्वरूप: | थीसिस |
| भाषा: | English |
| प्रकाशित: |
2022
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| विषय: | |
| ऑनलाइन पहुंच: | http://eprints.usm.my/59681/1/24%20Pages%20from%20CHEAH%20HONG%20LEONG%20-%20TESIS.pdf |
| _version_ | 1825908134034538496 |
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| author | Cheah, Hong Leong |
| author_facet | Cheah, Hong Leong |
| author_sort | Cheah, Hong Leong |
| collection | USM |
| description | Mycobacterium tuberculosis is an actinobacterium that causes chronic infections.
Its pathogenesis lies in the thick, lipid-rich cell envelope that facilitate persistence in
hostile environments. A refined knowledge of regulatory networks within M.
tuberculosis is important to understand its pathogenesis for therapeutic design. Small
regulatory RNAs (sRNAs) are key modulatory components within all organisms.
Extensive studies over the past decade have uncovered a plethora of bacterial sRNAs,
implying that bacterial transcriptomes are far more sophisticated than previously
anticipated. Here, sRNA profiles of M. tuberculosis exposed to different stresses
were established by analyzing RNA-seq data to address different facets of sRNAome
in M. tuberculosis. This study has identified a total of 1,376 sRNA candidates and
the subsequent 5’/3’RACE assay has successfully validated a total of five sRNAs,
among which predictedRNA_0578 may encode for a novel small peptide and
predictedRNA_0020 potentially affects the formation of mycobacterial cell wall.
Besides, this study also included transcription start site (TSS) landscapes that provide
additional transcriptional features, e.g., promoters and untranslated regions (UTRs).
Taken together, this study reveals the complexities of the mycobacterial sRNAome
and may facilitate the development of antisense antibiotics. |
| first_indexed | 2024-03-06T16:14:27Z |
| format | Thesis |
| id | usm.eprints-59681 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T16:14:27Z |
| publishDate | 2022 |
| record_format | dspace |
| spelling | usm.eprints-596812023-12-14T01:49:49Z http://eprints.usm.my/59681/ Transcription Start Site Mapping And Small Regulatory Rna Profiling Of Mycobacterium Tuberculosis Cheah, Hong Leong R5-920 Medicine (General) Mycobacterium tuberculosis is an actinobacterium that causes chronic infections. Its pathogenesis lies in the thick, lipid-rich cell envelope that facilitate persistence in hostile environments. A refined knowledge of regulatory networks within M. tuberculosis is important to understand its pathogenesis for therapeutic design. Small regulatory RNAs (sRNAs) are key modulatory components within all organisms. Extensive studies over the past decade have uncovered a plethora of bacterial sRNAs, implying that bacterial transcriptomes are far more sophisticated than previously anticipated. Here, sRNA profiles of M. tuberculosis exposed to different stresses were established by analyzing RNA-seq data to address different facets of sRNAome in M. tuberculosis. This study has identified a total of 1,376 sRNA candidates and the subsequent 5’/3’RACE assay has successfully validated a total of five sRNAs, among which predictedRNA_0578 may encode for a novel small peptide and predictedRNA_0020 potentially affects the formation of mycobacterial cell wall. Besides, this study also included transcription start site (TSS) landscapes that provide additional transcriptional features, e.g., promoters and untranslated regions (UTRs). Taken together, this study reveals the complexities of the mycobacterial sRNAome and may facilitate the development of antisense antibiotics. 2022-09 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/59681/1/24%20Pages%20from%20CHEAH%20HONG%20LEONG%20-%20TESIS.pdf Cheah, Hong Leong (2022) Transcription Start Site Mapping And Small Regulatory Rna Profiling Of Mycobacterium Tuberculosis. PhD thesis, Perpustakaan Hamzah Sendut. |
| spellingShingle | R5-920 Medicine (General) Cheah, Hong Leong Transcription Start Site Mapping And Small Regulatory Rna Profiling Of Mycobacterium Tuberculosis |
| title | Transcription Start Site Mapping And Small Regulatory Rna Profiling Of Mycobacterium Tuberculosis |
| title_full | Transcription Start Site Mapping And Small Regulatory Rna Profiling Of Mycobacterium Tuberculosis |
| title_fullStr | Transcription Start Site Mapping And Small Regulatory Rna Profiling Of Mycobacterium Tuberculosis |
| title_full_unstemmed | Transcription Start Site Mapping And Small Regulatory Rna Profiling Of Mycobacterium Tuberculosis |
| title_short | Transcription Start Site Mapping And Small Regulatory Rna Profiling Of Mycobacterium Tuberculosis |
| title_sort | transcription start site mapping and small regulatory rna profiling of mycobacterium tuberculosis |
| topic | R5-920 Medicine (General) |
| url | http://eprints.usm.my/59681/1/24%20Pages%20from%20CHEAH%20HONG%20LEONG%20-%20TESIS.pdf |
| work_keys_str_mv | AT cheahhongleong transcriptionstartsitemappingandsmallregulatoryrnaprofilingofmycobacteriumtuberculosis |