Development of a novel approach multiplex late-pcr electrochemical-enzyme based dna sensor for sequence specific detection of vibrio cholerae
Phase 1: Development of a Multiplex Linear-After-The-Exponential (LATE)- Polymerase Chain Reaction (PCR) • To design specific primers and optimize a multiplex LATE-PCR which is capable of simultaneously detecting the presence of V. cholerae, as well as cholera toxin and internal control genes....
| Main Author: | |
|---|---|
| Format: | Monograph |
| Language: | English |
| Published: |
Pusat Pengajian Kesihatan
2015
|
| Subjects: | |
| Online Access: | http://eprints.usm.my/59940/1/DR%20CHAN%20YEAN%20YEAN%20-%20e.pdf |
| _version_ | 1825908183215898624 |
|---|---|
| author | Yean, Chan Yean |
| author_facet | Yean, Chan Yean |
| author_sort | Yean, Chan Yean |
| collection | USM |
| description | Phase 1: Development of a Multiplex Linear-After-The-Exponential (LATE)- Polymerase Chain
Reaction (PCR)
• To design specific primers and optimize a multiplex LATE-PCR which is capable of
simultaneously detecting the presence of V. cholerae, as well as cholera toxin and internal control
genes.
Phase 2: Development of a Mixed Self-Assembled Monolayer (SAM) on Disposable Screen-Printed
Gold Electrode (SPGE)
• To design thiol-modified capture probe for sequence-specific hybridization with target LATEPCR
amplicons.
• To optimize the immobilization of capture probe DNAs on gold electrode surface using Self-
Assembled Monolayer (SAM) system.
Phase 3: Development of a Multiplex Electrochemical-Enzyme based DNA sensor
• To optimize the hybridization of labeled LATE-PCR amplicons to the capture probe DNAs.
• To optimize the simultaneous detection of multiple redox reactions catalyzed by alkaline
phosphatase and horseradish peroxidase by amperometry.
Phase 4: Evaluation of the Multiplex Electrochemical-Enzyme based DNA sensor
• To perform analytical evaluation of the multiplex electrochemical DNA hybridization
genosensor.
• To evaluate the performance of the multiplex electrochemical DNA hybridization genosensor
using clinical samples.
Objective Achieved
(Please state the extent to which the project objectives were achieved)
Phase 1: Development of a Multiplex Linear-After-The-Exponential (LATE)- Polymerase Chain
Reaction (PCR)
• To design specific primers and optimize a multiplex LATE-PCR which is capable of
simultaneously detecting the presence of V. cholerae, as well as cholera toxin and internal control
genes -100% achieved as per planned
Phase 2: Development of a Mixed Self-Assembled Monolayer (SAM) on Disposable Screen-Printed
Gold Electrode (SPGE)
• To design thiol-modified capture probe for sequence-specific hybridization with target LATEPCR
amplicons.-100% achieved as per planned
• To optimize the immobilization of capture probe DNAs on gold electrode surface using Self-
Assembled Monolayer (SAM) system -100% achieved as per planned
Phase 3: Development of a Multiplex Electrochemical-Enzyme based DNA sensor
• To optimize the hybridization of labeled LATE-PCR amplicons to the capture probe DNAs -
100% achieved as per planned
• To optimize the simultaneous detection of multiple redox reactions catalyzed by alkaline
phosphatase and horseradish peroxidase by amperometry -100% achieved as per planned
Phase 4: Evaluation of the Multiplex Electrochemical-Enzyme based DNA sensor
• To perform analytical evaluation of the multiplex electrochemical DNA hybridization
genosensor -100% achieved as per planned
• To evaluate the performance of the multiplex electrochemical DNA hybridization genosensor
using clinical samples -100% achieved as per planned |
| first_indexed | 2024-04-09T03:52:47Z |
| format | Monograph |
| id | usm.eprints-59940 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-04-09T03:52:47Z |
| publishDate | 2015 |
| publisher | Pusat Pengajian Kesihatan |
| record_format | dspace |
| spelling | usm.eprints-599402024-03-31T02:15:20Z http://eprints.usm.my/59940/ Development of a novel approach multiplex late-pcr electrochemical-enzyme based dna sensor for sequence specific detection of vibrio cholerae Yean, Chan Yean R Medicine RC Internal medicine Phase 1: Development of a Multiplex Linear-After-The-Exponential (LATE)- Polymerase Chain Reaction (PCR) • To design specific primers and optimize a multiplex LATE-PCR which is capable of simultaneously detecting the presence of V. cholerae, as well as cholera toxin and internal control genes. Phase 2: Development of a Mixed Self-Assembled Monolayer (SAM) on Disposable Screen-Printed Gold Electrode (SPGE) • To design thiol-modified capture probe for sequence-specific hybridization with target LATEPCR amplicons. • To optimize the immobilization of capture probe DNAs on gold electrode surface using Self- Assembled Monolayer (SAM) system. Phase 3: Development of a Multiplex Electrochemical-Enzyme based DNA sensor • To optimize the hybridization of labeled LATE-PCR amplicons to the capture probe DNAs. • To optimize the simultaneous detection of multiple redox reactions catalyzed by alkaline phosphatase and horseradish peroxidase by amperometry. Phase 4: Evaluation of the Multiplex Electrochemical-Enzyme based DNA sensor • To perform analytical evaluation of the multiplex electrochemical DNA hybridization genosensor. • To evaluate the performance of the multiplex electrochemical DNA hybridization genosensor using clinical samples. Objective Achieved (Please state the extent to which the project objectives were achieved) Phase 1: Development of a Multiplex Linear-After-The-Exponential (LATE)- Polymerase Chain Reaction (PCR) • To design specific primers and optimize a multiplex LATE-PCR which is capable of simultaneously detecting the presence of V. cholerae, as well as cholera toxin and internal control genes -100% achieved as per planned Phase 2: Development of a Mixed Self-Assembled Monolayer (SAM) on Disposable Screen-Printed Gold Electrode (SPGE) • To design thiol-modified capture probe for sequence-specific hybridization with target LATEPCR amplicons.-100% achieved as per planned • To optimize the immobilization of capture probe DNAs on gold electrode surface using Self- Assembled Monolayer (SAM) system -100% achieved as per planned Phase 3: Development of a Multiplex Electrochemical-Enzyme based DNA sensor • To optimize the hybridization of labeled LATE-PCR amplicons to the capture probe DNAs - 100% achieved as per planned • To optimize the simultaneous detection of multiple redox reactions catalyzed by alkaline phosphatase and horseradish peroxidase by amperometry -100% achieved as per planned Phase 4: Evaluation of the Multiplex Electrochemical-Enzyme based DNA sensor • To perform analytical evaluation of the multiplex electrochemical DNA hybridization genosensor -100% achieved as per planned • To evaluate the performance of the multiplex electrochemical DNA hybridization genosensor using clinical samples -100% achieved as per planned Pusat Pengajian Kesihatan 2015 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/59940/1/DR%20CHAN%20YEAN%20YEAN%20-%20e.pdf Yean, Chan Yean (2015) Development of a novel approach multiplex late-pcr electrochemical-enzyme based dna sensor for sequence specific detection of vibrio cholerae. Project Report. Pusat Pengajian Kesihatan. (Submitted) |
| spellingShingle | R Medicine RC Internal medicine Yean, Chan Yean Development of a novel approach multiplex late-pcr electrochemical-enzyme based dna sensor for sequence specific detection of vibrio cholerae |
| title | Development of a novel approach multiplex
late-pcr electrochemical-enzyme based dna
sensor for sequence specific detection of
vibrio cholerae |
| title_full | Development of a novel approach multiplex
late-pcr electrochemical-enzyme based dna
sensor for sequence specific detection of
vibrio cholerae |
| title_fullStr | Development of a novel approach multiplex
late-pcr electrochemical-enzyme based dna
sensor for sequence specific detection of
vibrio cholerae |
| title_full_unstemmed | Development of a novel approach multiplex
late-pcr electrochemical-enzyme based dna
sensor for sequence specific detection of
vibrio cholerae |
| title_short | Development of a novel approach multiplex
late-pcr electrochemical-enzyme based dna
sensor for sequence specific detection of
vibrio cholerae |
| title_sort | development of a novel approach multiplex late pcr electrochemical enzyme based dna sensor for sequence specific detection of vibrio cholerae |
| topic | R Medicine RC Internal medicine |
| url | http://eprints.usm.my/59940/1/DR%20CHAN%20YEAN%20YEAN%20-%20e.pdf |
| work_keys_str_mv | AT yeanchanyean developmentofanovelapproachmultiplexlatepcrelectrochemicalenzymebaseddnasensorforsequencespecificdetectionofvibriocholerae |