Analysis of total coliform and escherichia coli (e. coli) in freshwater and marine bivalves.
Bivalve molluscs commonly associated with many foodborne disease outbreaks. Total coliform and E. coli are always being used as microbial indicator to determine fecal pollution level in bivalves and water quality status. The objective of this study is to analyze the presence of total coliform and...
Main Author: | |
---|---|
Format: | Monograph |
Language: | English |
Published: |
Pusat Pengajian Kesihatan
2015
|
Subjects: | |
Online Access: | http://eprints.usm.my/60012/1/MOHAMAD%20SYAHMI%20ISKANDAR%20BIN%20NORLI%20-%20e.pdf |
_version_ | 1797014389037989888 |
---|---|
author | Norli, Mohamad Syahmi Iskandar |
author_facet | Norli, Mohamad Syahmi Iskandar |
author_sort | Norli, Mohamad Syahmi Iskandar |
collection | USM |
description | Bivalve molluscs commonly associated with many foodborne disease outbreaks. Total
coliform and E. coli are always being used as microbial indicator to determine fecal pollution
level in bivalves and water quality status. The objective of this study is to analyze the
presence of total coliform and Escherichia coli in selected freshwater bivalves and marine
bivalves. In this study, two freshwater bivalves: blood cockle (Anadara granosa) and Asian
clam (Corbicula fluminea) were selected while two marine bivalves involved in this study
were oysters (Crassostrea spp.) and carpet clam (Paphia textile}. Microbiological analyses
conducted through were conventional rapid test method by using 3M Petri film E.
co/z'/Coliform Count Plate. All samples were analyzed in term of raw or fresh sample and
cooked with different time interval. Results of these analyses showed that all raw samples
were contaminated with total coliform and E. coli. Total coliform and E. coli were also found
in lightly cooked bivalves (5 and 10 minutes cooking time) and not in fully cooked bivalves
(15 and 20 minutes cooking time) except for Anadara granosa and Paphia textile. Corbicula
fluminea identified as most contaminated bivalves as compared to other samples. In
conclusion, raw and lightly cooked bivalves are highly contaminated with pathogenic
bacteria and unsafe for human consumption. In order to reduce and eliminate number of
bacteria, bivalves must be cooked thoroughly with proper cooking temperature and method. |
first_indexed | 2024-04-09T03:52:53Z |
format | Monograph |
id | usm.eprints-60012 |
institution | Universiti Sains Malaysia |
language | English |
last_indexed | 2024-04-09T03:52:53Z |
publishDate | 2015 |
publisher | Pusat Pengajian Kesihatan |
record_format | dspace |
spelling | usm.eprints-600122024-04-04T05:05:03Z http://eprints.usm.my/60012/ Analysis of total coliform and escherichia coli (e. coli) in freshwater and marine bivalves. Norli, Mohamad Syahmi Iskandar RC109-216 Infectious and parasitic diseases Bivalve molluscs commonly associated with many foodborne disease outbreaks. Total coliform and E. coli are always being used as microbial indicator to determine fecal pollution level in bivalves and water quality status. The objective of this study is to analyze the presence of total coliform and Escherichia coli in selected freshwater bivalves and marine bivalves. In this study, two freshwater bivalves: blood cockle (Anadara granosa) and Asian clam (Corbicula fluminea) were selected while two marine bivalves involved in this study were oysters (Crassostrea spp.) and carpet clam (Paphia textile}. Microbiological analyses conducted through were conventional rapid test method by using 3M Petri film E. co/z'/Coliform Count Plate. All samples were analyzed in term of raw or fresh sample and cooked with different time interval. Results of these analyses showed that all raw samples were contaminated with total coliform and E. coli. Total coliform and E. coli were also found in lightly cooked bivalves (5 and 10 minutes cooking time) and not in fully cooked bivalves (15 and 20 minutes cooking time) except for Anadara granosa and Paphia textile. Corbicula fluminea identified as most contaminated bivalves as compared to other samples. In conclusion, raw and lightly cooked bivalves are highly contaminated with pathogenic bacteria and unsafe for human consumption. In order to reduce and eliminate number of bacteria, bivalves must be cooked thoroughly with proper cooking temperature and method. Pusat Pengajian Kesihatan 2015 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/60012/1/MOHAMAD%20SYAHMI%20ISKANDAR%20BIN%20NORLI%20-%20e.pdf Norli, Mohamad Syahmi Iskandar (2015) Analysis of total coliform and escherichia coli (e. coli) in freshwater and marine bivalves. Project Report. Pusat Pengajian Kesihatan. (Submitted) |
spellingShingle | RC109-216 Infectious and parasitic diseases Norli, Mohamad Syahmi Iskandar Analysis of total coliform and escherichia coli (e. coli) in freshwater and marine bivalves. |
title | Analysis of total coliform and escherichia
coli (e. coli) in freshwater and marine
bivalves. |
title_full | Analysis of total coliform and escherichia
coli (e. coli) in freshwater and marine
bivalves. |
title_fullStr | Analysis of total coliform and escherichia
coli (e. coli) in freshwater and marine
bivalves. |
title_full_unstemmed | Analysis of total coliform and escherichia
coli (e. coli) in freshwater and marine
bivalves. |
title_short | Analysis of total coliform and escherichia
coli (e. coli) in freshwater and marine
bivalves. |
title_sort | analysis of total coliform and escherichia coli e coli in freshwater and marine bivalves |
topic | RC109-216 Infectious and parasitic diseases |
url | http://eprints.usm.my/60012/1/MOHAMAD%20SYAHMI%20ISKANDAR%20BIN%20NORLI%20-%20e.pdf |
work_keys_str_mv | AT norlimohamadsyahmiiskandar analysisoftotalcoliformandescherichiacoliecoliinfreshwaterandmarinebivalves |