Ultraviolet Assisted Autolysis Of Sea Cucumber (Stichopus Horrens) Body Wall For The Enhancement Of Sulfated Polysaccharides Release
Sea cucumbers (Stichopus horrens) contain important bioactive compounds called sulfated polysaccharides (SP), which can be extracted by autolysis process utilizing its endogenous enzyme. However, this process is slow and not efficient. The aim of this study was to evaluate the mechanism of UV ass...
| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
2023
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| Subjects: | |
| Online Access: | http://eprints.usm.my/60013/1/NADA%20ITORUL%20UMAM%20-%20TESIS%27%20cut.pdf |
| _version_ | 1797014389242462208 |
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| author | Umam, Nada Itorul |
| author_facet | Umam, Nada Itorul |
| author_sort | Umam, Nada Itorul |
| collection | USM |
| description | Sea cucumbers (Stichopus horrens) contain important bioactive compounds
called sulfated polysaccharides (SP), which can be extracted by autolysis process
utilizing its endogenous enzyme. However, this process is slow and not efficient. The
aim of this study was to evaluate the mechanism of UV assisted autolysis in enhancing
the release of SP from sea cucumber body wall. In phase I, the effects of UV dosage
and pH on endogenous proteases activity, biochemical, and structural changes of
SHBW during autolysis were evaluated. Frozen SHBW was homogenized with and
without buffer (pH 6, 7, 8) and exposed to low (10,800 J/m2) and high (324,000 J/m2)
UV dosage prior to autolysis at 50 °C for 4 and 8 hours. The endogenous protease
activity and respective biochemical changes were evaluated from the soluble fraction
while the insoluble fraction of SHBW was evaluated for secondary protein structural
changes and thermal stability using Fourier-transform Infrared Spectroscopy (FTIR),
Differential Scanning Calorimetry (DSC), and Thermogravimetry Analysis (TGA).
Increase in UV dosage by 30x, increased proteolytic activity by 4.5-fold after 4 hours
autolysis, resulting in increased soluble protein, hydroxyproline, and
glycosaminoglycan (GAG) content by 5, 4, and 3 folds, respectively. Although sample
without buffer showed similar endogenous protease activity with control sample, the
total soluble protein, hydroxyproline, and GAG were higher by 4, 0.1, and 2.5 folds,
respectively. |
| first_indexed | 2024-03-06T16:15:14Z |
| format | Thesis |
| id | usm.eprints-60013 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-03-06T16:15:14Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | usm.eprints-600132024-02-27T07:59:38Z http://eprints.usm.my/60013/ Ultraviolet Assisted Autolysis Of Sea Cucumber (Stichopus Horrens) Body Wall For The Enhancement Of Sulfated Polysaccharides Release Umam, Nada Itorul TP368-456 Food processing and manufacture Sea cucumbers (Stichopus horrens) contain important bioactive compounds called sulfated polysaccharides (SP), which can be extracted by autolysis process utilizing its endogenous enzyme. However, this process is slow and not efficient. The aim of this study was to evaluate the mechanism of UV assisted autolysis in enhancing the release of SP from sea cucumber body wall. In phase I, the effects of UV dosage and pH on endogenous proteases activity, biochemical, and structural changes of SHBW during autolysis were evaluated. Frozen SHBW was homogenized with and without buffer (pH 6, 7, 8) and exposed to low (10,800 J/m2) and high (324,000 J/m2) UV dosage prior to autolysis at 50 °C for 4 and 8 hours. The endogenous protease activity and respective biochemical changes were evaluated from the soluble fraction while the insoluble fraction of SHBW was evaluated for secondary protein structural changes and thermal stability using Fourier-transform Infrared Spectroscopy (FTIR), Differential Scanning Calorimetry (DSC), and Thermogravimetry Analysis (TGA). Increase in UV dosage by 30x, increased proteolytic activity by 4.5-fold after 4 hours autolysis, resulting in increased soluble protein, hydroxyproline, and glycosaminoglycan (GAG) content by 5, 4, and 3 folds, respectively. Although sample without buffer showed similar endogenous protease activity with control sample, the total soluble protein, hydroxyproline, and GAG were higher by 4, 0.1, and 2.5 folds, respectively. 2023-04 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/60013/1/NADA%20ITORUL%20UMAM%20-%20TESIS%27%20cut.pdf Umam, Nada Itorul (2023) Ultraviolet Assisted Autolysis Of Sea Cucumber (Stichopus Horrens) Body Wall For The Enhancement Of Sulfated Polysaccharides Release. Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | TP368-456 Food processing and manufacture Umam, Nada Itorul Ultraviolet Assisted Autolysis Of Sea Cucumber (Stichopus Horrens) Body Wall For The Enhancement Of Sulfated Polysaccharides Release |
| title | Ultraviolet Assisted Autolysis Of Sea Cucumber (Stichopus Horrens) Body Wall For The Enhancement Of Sulfated Polysaccharides Release |
| title_full | Ultraviolet Assisted Autolysis Of Sea Cucumber (Stichopus Horrens) Body Wall For The Enhancement Of Sulfated Polysaccharides Release |
| title_fullStr | Ultraviolet Assisted Autolysis Of Sea Cucumber (Stichopus Horrens) Body Wall For The Enhancement Of Sulfated Polysaccharides Release |
| title_full_unstemmed | Ultraviolet Assisted Autolysis Of Sea Cucumber (Stichopus Horrens) Body Wall For The Enhancement Of Sulfated Polysaccharides Release |
| title_short | Ultraviolet Assisted Autolysis Of Sea Cucumber (Stichopus Horrens) Body Wall For The Enhancement Of Sulfated Polysaccharides Release |
| title_sort | ultraviolet assisted autolysis of sea cucumber stichopus horrens body wall for the enhancement of sulfated polysaccharides release |
| topic | TP368-456 Food processing and manufacture |
| url | http://eprints.usm.my/60013/1/NADA%20ITORUL%20UMAM%20-%20TESIS%27%20cut.pdf |
| work_keys_str_mv | AT umamnadaitorul ultravioletassistedautolysisofseacucumberstichopushorrensbodywallfortheenhancementofsulfatedpolysaccharidesrelease |