| Summary: | Most breast cancer tissue contain the region with low level of oxygenic
measurement or known as hypoxic region. Hypoxic region known to express large
quantities of hypoxic marker, HIF-la. This hypoxic condition hinders the effectiveness of
treatment but also promoting cancer aggressiveness due to transcriptional up-regulation of
metastatic related genes. Metastatic in breast cancer was also known to have involved with
ion channel mainly the VGCSs. In this study, it was hypothesized that HIF1 alpha regulate VGSC expression which promote in breast cancer metastasis. Therefore, this study was done to investigate the interaction between HIF-laand VGSCs particularly Navi.5, and its
neonatal splicing form, nNavl.5 in breast cancer cells using siRNA in MDA-MB 231 cell
line. siRNA was conducted to knock-down HIF-la and the mRNA expression level of
HIF-la, Nav 1.5 and nNavl.5 were measured using real-time PCR. When HIF-la was
knock-downed with the siRNA, the expression of Navi.5 and nNav 1.5 was reduce by
11%. The -30% and result conclude the possible relationship between HIF-la and
VGSCs mainly Navi.5 and nNavl.5 in MDA-MB 231 breast cancer cells. Hence, by
understanding the involvement of HIF-la VGSCs mainly Navi.5 and nNavl.5 could
promise useful information in breast cancer metastasis.
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