Effects of knocking-down HIF-la (siRNA) on vgsc expression in the aggressive human breast cancer cells (MDA-MB-231)
Most breast cancer tissue contain the region with low level of oxygenic measurement or known as hypoxic region. Hypoxic region known to express large quantities of hypoxic marker, HIF-la. This hypoxic condition hinders the effectiveness of treatment but also promoting cancer aggressiveness due to...
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| Format: | Monograph |
| Language: | English |
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Pusat Pengajian Kesihatan
2015
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| Online Access: | http://eprints.usm.my/60017/1/MOHD%20ZAKI%20BIN%20HUSSIN%20-%20e.pdf |
| _version_ | 1825908197869748224 |
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| author | Hussin, Mohd Zaki |
| author_facet | Hussin, Mohd Zaki |
| author_sort | Hussin, Mohd Zaki |
| collection | USM |
| description | Most breast cancer tissue contain the region with low level of oxygenic
measurement or known as hypoxic region. Hypoxic region known to express large
quantities of hypoxic marker, HIF-la. This hypoxic condition hinders the effectiveness of
treatment but also promoting cancer aggressiveness due to transcriptional up-regulation of
metastatic related genes. Metastatic in breast cancer was also known to have involved with
ion channel mainly the VGCSs. In this study, it was hypothesized that HIF1 alpha regulate VGSC expression which promote in breast cancer metastasis. Therefore, this study was done to investigate the interaction between HIF-laand VGSCs particularly Navi.5, and its
neonatal splicing form, nNavl.5 in breast cancer cells using siRNA in MDA-MB 231 cell
line. siRNA was conducted to knock-down HIF-la and the mRNA expression level of
HIF-la, Nav 1.5 and nNavl.5 were measured using real-time PCR. When HIF-la was
knock-downed with the siRNA, the expression of Navi.5 and nNav 1.5 was reduce by
11%. The -30% and result conclude the possible relationship between HIF-la and
VGSCs mainly Navi.5 and nNavl.5 in MDA-MB 231 breast cancer cells. Hence, by
understanding the involvement of HIF-la VGSCs mainly Navi.5 and nNavl.5 could
promise useful information in breast cancer metastasis. |
| first_indexed | 2024-04-09T03:52:53Z |
| format | Monograph |
| id | usm.eprints-60017 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-04-09T03:52:53Z |
| publishDate | 2015 |
| publisher | Pusat Pengajian Kesihatan |
| record_format | dspace |
| spelling | usm.eprints-600172024-04-07T03:07:42Z http://eprints.usm.my/60017/ Effects of knocking-down HIF-la (siRNA) on vgsc expression in the aggressive human breast cancer cells (MDA-MB-231) Hussin, Mohd Zaki RA440-440.87 Study and teaching. Research RC254-282 Neoplasms. Tumors. Oncology (including Cancer) Most breast cancer tissue contain the region with low level of oxygenic measurement or known as hypoxic region. Hypoxic region known to express large quantities of hypoxic marker, HIF-la. This hypoxic condition hinders the effectiveness of treatment but also promoting cancer aggressiveness due to transcriptional up-regulation of metastatic related genes. Metastatic in breast cancer was also known to have involved with ion channel mainly the VGCSs. In this study, it was hypothesized that HIF1 alpha regulate VGSC expression which promote in breast cancer metastasis. Therefore, this study was done to investigate the interaction between HIF-laand VGSCs particularly Navi.5, and its neonatal splicing form, nNavl.5 in breast cancer cells using siRNA in MDA-MB 231 cell line. siRNA was conducted to knock-down HIF-la and the mRNA expression level of HIF-la, Nav 1.5 and nNavl.5 were measured using real-time PCR. When HIF-la was knock-downed with the siRNA, the expression of Navi.5 and nNav 1.5 was reduce by 11%. The -30% and result conclude the possible relationship between HIF-la and VGSCs mainly Navi.5 and nNavl.5 in MDA-MB 231 breast cancer cells. Hence, by understanding the involvement of HIF-la VGSCs mainly Navi.5 and nNavl.5 could promise useful information in breast cancer metastasis. Pusat Pengajian Kesihatan 2015 Monograph NonPeerReviewed application/pdf en http://eprints.usm.my/60017/1/MOHD%20ZAKI%20BIN%20HUSSIN%20-%20e.pdf Hussin, Mohd Zaki (2015) Effects of knocking-down HIF-la (siRNA) on vgsc expression in the aggressive human breast cancer cells (MDA-MB-231). Project Report. Pusat Pengajian Kesihatan. (Submitted) |
| spellingShingle | RA440-440.87 Study and teaching. Research RC254-282 Neoplasms. Tumors. Oncology (including Cancer) Hussin, Mohd Zaki Effects of knocking-down HIF-la (siRNA) on vgsc expression in the aggressive human breast cancer cells (MDA-MB-231) |
| title | Effects of knocking-down HIF-la (siRNA) on vgsc expression in the aggressive human breast cancer cells (MDA-MB-231) |
| title_full | Effects of knocking-down HIF-la (siRNA) on vgsc expression in the aggressive human breast cancer cells (MDA-MB-231) |
| title_fullStr | Effects of knocking-down HIF-la (siRNA) on vgsc expression in the aggressive human breast cancer cells (MDA-MB-231) |
| title_full_unstemmed | Effects of knocking-down HIF-la (siRNA) on vgsc expression in the aggressive human breast cancer cells (MDA-MB-231) |
| title_short | Effects of knocking-down HIF-la (siRNA) on vgsc expression in the aggressive human breast cancer cells (MDA-MB-231) |
| title_sort | effects of knocking down hif la sirna on vgsc expression in the aggressive human breast cancer cells mda mb 231 |
| topic | RA440-440.87 Study and teaching. Research RC254-282 Neoplasms. Tumors. Oncology (including Cancer) |
| url | http://eprints.usm.my/60017/1/MOHD%20ZAKI%20BIN%20HUSSIN%20-%20e.pdf |
| work_keys_str_mv | AT hussinmohdzaki effectsofknockingdownhiflasirnaonvgscexpressionintheaggressivehumanbreastcancercellsmdamb231 |