Short term effect of human lymphocytes viability upon exposure of products with parabens
Personal care products are commonly used in personal hygiene and constitutes of broad classes of chemical compounds. Since 1930s, paraben have been used in cosmetic as preservatives. However, paraben effect has become controversial due to report on estrogenic activity. The aims of this study are...
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| Format: | Monograph |
| Language: | English |
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Universiti Sains Malaysia
2015
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| Online Access: | http://eprints.usm.my/60414/1/NURHIDASAZLINA%20BINTI%20SAABAN%20-%20e.pdf |
| Summary: | Personal care products are commonly used in personal hygiene and constitutes of
broad classes of chemical compounds. Since 1930s, paraben have been used in
cosmetic as preservatives. However, paraben effect has become controversial due to
report on estrogenic activity. The aims of this study are to determine the effect of
lymphocyte viability and morphological after being administered products with
paraben at different concentrations for short term exposure. Blood sample were drew
from seven healthy subjects. Lymphocytes were isolated using EasySep Direct
Human Total Lymphocyte Isolation Kit. The MTT assay was used to determine
lymphocytes viability. Blood smear and stained with Wright's stain were used to
determine the morphological changes of the lymphocytes. The result showed
significant (p < 0.001) and moderate positive (r = 0.351) correlation between paraben
concentration with the percentage of lymphocyte viability. The cell viability recorded
for methyl paraben was up to 120%. The lymphocytes rapidly proliferate due to
antigen that bind to the receptors and activates the lymphocytes. This showed that
products with paraben have toxicity effects towards lymphocytes even after being
exposed in short period of time. |
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