Biotransformation Of Ferulic Acid Extracted From Biomass To Biovanillin By Amycolatopsis Sp. Atcc 39116
This study focuses on the biotransformation of synthetic and extracted ferulic acid (FA) from lignocellulosic biomass using growing cells of Amyco/alopsis sp. ATCC 39116 as a biocatalyst. Preliminary alkaline hydrolysis showed that the hydrolyzate from oil palm empty fruit bunch (OPEFB) contained...
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| Format: | Thesis |
| Language: | English |
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2014
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| Online Access: | http://eprints.usm.my/60852/1/24%20Pages%20from%2000001785213.pdf |
| _version_ | 1825908360473477120 |
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| author | Abu Sepian, Noor Raihana |
| author_facet | Abu Sepian, Noor Raihana |
| author_sort | Abu Sepian, Noor Raihana |
| collection | USM |
| description | This study focuses on the biotransformation of synthetic and extracted ferulic
acid (FA) from lignocellulosic biomass using growing cells of Amyco/alopsis sp.
ATCC 39116 as a biocatalyst. Preliminary alkaline hydrolysis showed that the
hydrolyzate from oil palm empty fruit bunch (OPEFB) contained the highest amount
of FA (199.4 mg/l) among the other three lignocellulosic wastes (i.e. oil palm frond,
coconut fiber and sheIl). Optimization of extraction was carried out using Response
Surface Methodology (RSM) via Central Composite Design (CCD) on the effect of
three independent variables, i.e. concentration of NaOH (1.5-5 M), solid/liquid ratio
(0.03-0.084 g/ml) and time (16-32 h). The optimum FA concentration of 2.04 mg/g
OPEFB (365.19 mg/l) was obtained under the optimal conditions of 1.5 M ofNaOH
concentration, 0.03 g/ml of solid/liquid ratio and 32 h of extraction time. The
extracted FA was further purified using polyvinylpolypyrrolidone (PVPP) and
activated carbon, and about 97.26% was successfully recovered from the extract
using PVPP. The partially purified FA was converted into 38.47% molar yield of
vanillin, showing the ability of Amyco/alopsis sp. ATCC 39116 to convert the
extracted FA from lignocellulosic waste. |
| first_indexed | 2024-09-25T03:57:22Z |
| format | Thesis |
| id | usm.eprints-60852 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-09-25T03:57:22Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | usm.eprints-608522024-07-18T02:15:33Z http://eprints.usm.my/60852/ Biotransformation Of Ferulic Acid Extracted From Biomass To Biovanillin By Amycolatopsis Sp. Atcc 39116 Abu Sepian, Noor Raihana TP1-1185 Chemical technology This study focuses on the biotransformation of synthetic and extracted ferulic acid (FA) from lignocellulosic biomass using growing cells of Amyco/alopsis sp. ATCC 39116 as a biocatalyst. Preliminary alkaline hydrolysis showed that the hydrolyzate from oil palm empty fruit bunch (OPEFB) contained the highest amount of FA (199.4 mg/l) among the other three lignocellulosic wastes (i.e. oil palm frond, coconut fiber and sheIl). Optimization of extraction was carried out using Response Surface Methodology (RSM) via Central Composite Design (CCD) on the effect of three independent variables, i.e. concentration of NaOH (1.5-5 M), solid/liquid ratio (0.03-0.084 g/ml) and time (16-32 h). The optimum FA concentration of 2.04 mg/g OPEFB (365.19 mg/l) was obtained under the optimal conditions of 1.5 M ofNaOH concentration, 0.03 g/ml of solid/liquid ratio and 32 h of extraction time. The extracted FA was further purified using polyvinylpolypyrrolidone (PVPP) and activated carbon, and about 97.26% was successfully recovered from the extract using PVPP. The partially purified FA was converted into 38.47% molar yield of vanillin, showing the ability of Amyco/alopsis sp. ATCC 39116 to convert the extracted FA from lignocellulosic waste. 2014-11 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/60852/1/24%20Pages%20from%2000001785213.pdf Abu Sepian, Noor Raihana (2014) Biotransformation Of Ferulic Acid Extracted From Biomass To Biovanillin By Amycolatopsis Sp. Atcc 39116. Masters thesis, Perpustakaan Hamzah Sendut. |
| spellingShingle | TP1-1185 Chemical technology Abu Sepian, Noor Raihana Biotransformation Of Ferulic Acid Extracted From Biomass To Biovanillin By Amycolatopsis Sp. Atcc 39116 |
| title | Biotransformation Of Ferulic Acid Extracted From Biomass To Biovanillin By Amycolatopsis Sp. Atcc 39116 |
| title_full | Biotransformation Of Ferulic Acid Extracted From Biomass To Biovanillin By Amycolatopsis Sp. Atcc 39116 |
| title_fullStr | Biotransformation Of Ferulic Acid Extracted From Biomass To Biovanillin By Amycolatopsis Sp. Atcc 39116 |
| title_full_unstemmed | Biotransformation Of Ferulic Acid Extracted From Biomass To Biovanillin By Amycolatopsis Sp. Atcc 39116 |
| title_short | Biotransformation Of Ferulic Acid Extracted From Biomass To Biovanillin By Amycolatopsis Sp. Atcc 39116 |
| title_sort | biotransformation of ferulic acid extracted from biomass to biovanillin by amycolatopsis sp atcc 39116 |
| topic | TP1-1185 Chemical technology |
| url | http://eprints.usm.my/60852/1/24%20Pages%20from%2000001785213.pdf |
| work_keys_str_mv | AT abusepiannoorraihana biotransformationofferulicacidextractedfrombiomasstobiovanillinbyamycolatopsisspatcc39116 |