Effects Of Human Umbilical Cord-Derived Mesenchymal Stem Cells On Human Corneal Epithelial Cells’ Growth
Ex vivo cultivated corneal limbal stem cell (LSC) transplantation is the contemporary therapy for comeal epithelial regeneration. The success rate of this therapy is dependent on the cell culture conditions and the carrier system. Human umbilical cord mesenchymal stem cells (hUC-MSCs) has regener...
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| Format: | Thesis |
| Language: | English |
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2019
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| Online Access: | http://eprints.usm.my/61199/1/Effect%20of%20human%20umbilical%20cut.pdf |
| _version_ | 1811138942977703936 |
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| author | Azmi, Siti Maisura |
| author_facet | Azmi, Siti Maisura |
| author_sort | Azmi, Siti Maisura |
| collection | USM |
| description | Ex vivo cultivated corneal limbal stem cell (LSC) transplantation is the
contemporary therapy for comeal epithelial regeneration. The success rate of this
therapy is dependent on the cell culture conditions and the carrier system. Human
umbilical cord mesenchymal stem cells (hUC-MSCs) has regenerative ability and
are rich with paracrine factors. Cells cultivated in spherical scaffolds could provide
a similar in vivo environment to the cell niche and may positively alter the stem cells
properties. This study aims to improve the cellular quality of the comeal epithelial
transplantation graft using a robust cell population method. Human telomere
modified comeal epithelial cells (hTCECs) were co-cultured in direct and indirect
cell culture with hUC-MSCs. Characterisation of corneal markers p63, ABCG2,
ABCB5, and differentiation marker K3 were performed. |
| first_indexed | 2024-09-25T03:58:13Z |
| format | Thesis |
| id | usm.eprints-61199 |
| institution | Universiti Sains Malaysia |
| language | English |
| last_indexed | 2024-09-25T03:58:13Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | usm.eprints-611992024-09-23T03:14:22Z http://eprints.usm.my/61199/ Effects Of Human Umbilical Cord-Derived Mesenchymal Stem Cells On Human Corneal Epithelial Cells’ Growth Azmi, Siti Maisura RE Ophthalmology Ex vivo cultivated corneal limbal stem cell (LSC) transplantation is the contemporary therapy for comeal epithelial regeneration. The success rate of this therapy is dependent on the cell culture conditions and the carrier system. Human umbilical cord mesenchymal stem cells (hUC-MSCs) has regenerative ability and are rich with paracrine factors. Cells cultivated in spherical scaffolds could provide a similar in vivo environment to the cell niche and may positively alter the stem cells properties. This study aims to improve the cellular quality of the comeal epithelial transplantation graft using a robust cell population method. Human telomere modified comeal epithelial cells (hTCECs) were co-cultured in direct and indirect cell culture with hUC-MSCs. Characterisation of corneal markers p63, ABCG2, ABCB5, and differentiation marker K3 were performed. 2019-12 Thesis NonPeerReviewed application/pdf en http://eprints.usm.my/61199/1/Effect%20of%20human%20umbilical%20cut.pdf Azmi, Siti Maisura (2019) Effects Of Human Umbilical Cord-Derived Mesenchymal Stem Cells On Human Corneal Epithelial Cells’ Growth. Masters thesis, Universiti Sains Malaysia. |
| spellingShingle | RE Ophthalmology Azmi, Siti Maisura Effects Of Human Umbilical Cord-Derived Mesenchymal Stem Cells On Human Corneal Epithelial Cells’ Growth |
| title | Effects Of Human Umbilical Cord-Derived
Mesenchymal Stem Cells On
Human Corneal Epithelial Cells’
Growth |
| title_full | Effects Of Human Umbilical Cord-Derived
Mesenchymal Stem Cells On
Human Corneal Epithelial Cells’
Growth |
| title_fullStr | Effects Of Human Umbilical Cord-Derived
Mesenchymal Stem Cells On
Human Corneal Epithelial Cells’
Growth |
| title_full_unstemmed | Effects Of Human Umbilical Cord-Derived
Mesenchymal Stem Cells On
Human Corneal Epithelial Cells’
Growth |
| title_short | Effects Of Human Umbilical Cord-Derived
Mesenchymal Stem Cells On
Human Corneal Epithelial Cells’
Growth |
| title_sort | effects of human umbilical cord derived mesenchymal stem cells on human corneal epithelial cells growth |
| topic | RE Ophthalmology |
| url | http://eprints.usm.my/61199/1/Effect%20of%20human%20umbilical%20cut.pdf |
| work_keys_str_mv | AT azmisitimaisura effectsofhumanumbilicalcordderivedmesenchymalstemcellsonhumancornealepithelialcellsgrowth |