In vitro surface efficacy of CaP-based anodised titanium for bone implants
This study examined the effect of changes to the surface of CaP-based anodised titanium on osteogenesis and antimicrobial activity in vitro. The present work is proposed to investigate the mechanical stability of two different CaP/TiO2 micromorphology-based coatings, namely donut-shaped (350 V) and...
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| Format: | Article |
| Language: | English |
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Elsevier
2023
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| Online Access: | http://eprints.uthm.edu.my/10009/1/J16043_3ebf01813a855fc8bfbea2ea2e595eb8.pdf |
| _version_ | 1796870077641916416 |
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| author | Alipal, J. Saidin, S. Kai Lo, A.Z. Koshy, P. Abdullah, H.Z. Idris, M.I. Lee, T.C. |
| author_facet | Alipal, J. Saidin, S. Kai Lo, A.Z. Koshy, P. Abdullah, H.Z. Idris, M.I. Lee, T.C. |
| author_sort | Alipal, J. |
| collection | UTHM |
| description | This study examined the effect of changes to the surface of CaP-based anodised titanium on osteogenesis and
antimicrobial activity in vitro. The present work is proposed to investigate the mechanical stability of two different CaP/TiO2 micromorphology-based coatings, namely donut-shaped (350 V) and needle-shaped (450 V), that are fabricated by single-step micro-arc anodic oxidation at different conditions alongside with their cytocompatibility and antibacterial properties. The microhardness of the sample anodised at 450 V is ~100 MPa lower than that seen in the sample fabricated at 350 V, but both resultant coatings were strongly adhere to the substrate. However, the coating at 450 V was observed to suffer from delamination, indicating the needle-like
structure is mechanically unstable. After 7 days of SBF immersion, the 450 V sample was highly bioactive relative to the 350 V sample. However, MTT assays showed that the 450 V coatings showed lower growth rate of hFOB 1.19 at day 14, which indicates that although the structure does have a superior ability to form apatite, it has long-term negative implications possibly from the toxicity of the structure to bone growth. The Alizarin-red staining shows that 450 V sample shows a negative trend of osteogenic mineralisation, thus qualitatively validating the toxicity. For antimicrobial effects, the donut-shaped microstructures were shown to have adequate antibacterial properties compared to the needle-like structures. Overall, this study suggest that the donut-shaped TiO2 morphology is the best cementless interface for bone cell anchorage. |
| first_indexed | 2024-03-05T22:04:12Z |
| format | Article |
| id | uthm.eprints-10009 |
| institution | Universiti Tun Hussein Onn Malaysia |
| language | English |
| last_indexed | 2024-03-05T22:04:12Z |
| publishDate | 2023 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | uthm.eprints-100092023-09-25T01:47:15Z http://eprints.uthm.edu.my/10009/ In vitro surface efficacy of CaP-based anodised titanium for bone implants Alipal, J. Saidin, S. Kai Lo, A.Z. Koshy, P. Abdullah, H.Z. Idris, M.I. Lee, T.C. TA Engineering (General). Civil engineering (General) This study examined the effect of changes to the surface of CaP-based anodised titanium on osteogenesis and antimicrobial activity in vitro. The present work is proposed to investigate the mechanical stability of two different CaP/TiO2 micromorphology-based coatings, namely donut-shaped (350 V) and needle-shaped (450 V), that are fabricated by single-step micro-arc anodic oxidation at different conditions alongside with their cytocompatibility and antibacterial properties. The microhardness of the sample anodised at 450 V is ~100 MPa lower than that seen in the sample fabricated at 350 V, but both resultant coatings were strongly adhere to the substrate. However, the coating at 450 V was observed to suffer from delamination, indicating the needle-like structure is mechanically unstable. After 7 days of SBF immersion, the 450 V sample was highly bioactive relative to the 350 V sample. However, MTT assays showed that the 450 V coatings showed lower growth rate of hFOB 1.19 at day 14, which indicates that although the structure does have a superior ability to form apatite, it has long-term negative implications possibly from the toxicity of the structure to bone growth. The Alizarin-red staining shows that 450 V sample shows a negative trend of osteogenic mineralisation, thus qualitatively validating the toxicity. For antimicrobial effects, the donut-shaped microstructures were shown to have adequate antibacterial properties compared to the needle-like structures. Overall, this study suggest that the donut-shaped TiO2 morphology is the best cementless interface for bone cell anchorage. Elsevier 2023 Article PeerReviewed text en http://eprints.uthm.edu.my/10009/1/J16043_3ebf01813a855fc8bfbea2ea2e595eb8.pdf Alipal, J. and Saidin, S. and Kai Lo, A.Z. and Koshy, P. and Abdullah, H.Z. and Idris, M.I. and Lee, T.C. (2023) In vitro surface efficacy of CaP-based anodised titanium for bone implants. Surfaces and Interfaces, 39. pp. 1-21. https://doi.org/10.1016/j.surfin.2023.102872 |
| spellingShingle | TA Engineering (General). Civil engineering (General) Alipal, J. Saidin, S. Kai Lo, A.Z. Koshy, P. Abdullah, H.Z. Idris, M.I. Lee, T.C. In vitro surface efficacy of CaP-based anodised titanium for bone implants |
| title | In vitro surface efficacy of CaP-based anodised titanium for bone implants |
| title_full | In vitro surface efficacy of CaP-based anodised titanium for bone implants |
| title_fullStr | In vitro surface efficacy of CaP-based anodised titanium for bone implants |
| title_full_unstemmed | In vitro surface efficacy of CaP-based anodised titanium for bone implants |
| title_short | In vitro surface efficacy of CaP-based anodised titanium for bone implants |
| title_sort | in vitro surface efficacy of cap based anodised titanium for bone implants |
| topic | TA Engineering (General). Civil engineering (General) |
| url | http://eprints.uthm.edu.my/10009/1/J16043_3ebf01813a855fc8bfbea2ea2e595eb8.pdf |
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