In vitro surface efficacy of CaP-based anodised titanium for bone implants
This study examined the effect of changes to the surface of CaP-based anodised titanium on osteogenesis and antimicrobial activity in vitro. The present work is proposed to investigate the mechanical stability of two different CaP/TiO2 micromorphology-based coatings, namely donut-shaped (350 V) and...
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| Format: | Article |
| Language: | English |
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Elsevier
2023
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| Online Access: | http://eprints.uthm.edu.my/11521/1/J16043_3ebf01813a855fc8bfbea2ea2e595eb8.pdf |
| _version_ | 1811134802166808576 |
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| author | Alipal, J. Saidin, S. Kai Lo, A.Z. Koshy, P. Abdullah, H.Z. Idris, M.I. Lee, T.C. |
| author_facet | Alipal, J. Saidin, S. Kai Lo, A.Z. Koshy, P. Abdullah, H.Z. Idris, M.I. Lee, T.C. |
| author_sort | Alipal, J. |
| collection | UTHM |
| description | This study examined the effect of changes to the surface of CaP-based anodised titanium on osteogenesis and antimicrobial activity in vitro. The present work is proposed to investigate the mechanical stability of two different CaP/TiO2 micromorphology-based coatings, namely donut-shaped (350 V) and needle-shaped (450 V), that are fabricated by single-step micro-arc anodic oxidation at different conditions alongside with their cytocompatibility and antibacterial properties. The microhardness of the sample anodised at 450 V is ~100 MPa lower than that seen in the sample fabricated at 350 V, but both resultant coatings were strongly adhere to the substrate. However, the coating at 450 V was observed to suffer from delamination, indicating the needle-like
structure is mechanically unstable. After 7 days of SBF immersion, the 450 V sample was highly bioactive relative to the 350 V sample. However, MTT assays showed that the 450 V coatings showed lower growth rate of hFOB 1.19 at day 14, which indicates that although the structure does have a superior ability to form apatite, it has long-term negative implications possibly from the toxicity of the structure to bone growth. The Alizarin-red staining shows that 450 V sample shows a negative trend of osteogenic mineralisation, thus qualitatively validating the toxicity. For antimicrobial effects, the donut-shaped microstructures were shown to have adequate antibacterial properties compared to the needle-like structures. Overall, this study suggest that the donut-shaped TiO2 morphology is the best cementless interface for bone cell anchorage. |
| first_indexed | 2024-09-24T00:10:56Z |
| format | Article |
| id | uthm.eprints-11521 |
| institution | Universiti Tun Hussein Onn Malaysia |
| language | English |
| last_indexed | 2024-09-24T00:10:56Z |
| publishDate | 2023 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | uthm.eprints-115212024-08-12T01:49:34Z http://eprints.uthm.edu.my/11521/ In vitro surface efficacy of CaP-based anodised titanium for bone implants Alipal, J. Saidin, S. Kai Lo, A.Z. Koshy, P. Abdullah, H.Z. Idris, M.I. Lee, T.C. T Technology (General) This study examined the effect of changes to the surface of CaP-based anodised titanium on osteogenesis and antimicrobial activity in vitro. The present work is proposed to investigate the mechanical stability of two different CaP/TiO2 micromorphology-based coatings, namely donut-shaped (350 V) and needle-shaped (450 V), that are fabricated by single-step micro-arc anodic oxidation at different conditions alongside with their cytocompatibility and antibacterial properties. The microhardness of the sample anodised at 450 V is ~100 MPa lower than that seen in the sample fabricated at 350 V, but both resultant coatings were strongly adhere to the substrate. However, the coating at 450 V was observed to suffer from delamination, indicating the needle-like structure is mechanically unstable. After 7 days of SBF immersion, the 450 V sample was highly bioactive relative to the 350 V sample. However, MTT assays showed that the 450 V coatings showed lower growth rate of hFOB 1.19 at day 14, which indicates that although the structure does have a superior ability to form apatite, it has long-term negative implications possibly from the toxicity of the structure to bone growth. The Alizarin-red staining shows that 450 V sample shows a negative trend of osteogenic mineralisation, thus qualitatively validating the toxicity. For antimicrobial effects, the donut-shaped microstructures were shown to have adequate antibacterial properties compared to the needle-like structures. Overall, this study suggest that the donut-shaped TiO2 morphology is the best cementless interface for bone cell anchorage. Elsevier 2023 Article PeerReviewed text en http://eprints.uthm.edu.my/11521/1/J16043_3ebf01813a855fc8bfbea2ea2e595eb8.pdf Alipal, J. and Saidin, S. and Kai Lo, A.Z. and Koshy, P. and Abdullah, H.Z. and Idris, M.I. and Lee, T.C. (2023) In vitro surface efficacy of CaP-based anodised titanium for bone implants. Surfaces and Interfaces, 39. pp. 1-21. https://doi.org/10.1016/j.surfin.2023.102872 |
| spellingShingle | T Technology (General) Alipal, J. Saidin, S. Kai Lo, A.Z. Koshy, P. Abdullah, H.Z. Idris, M.I. Lee, T.C. In vitro surface efficacy of CaP-based anodised titanium for bone implants |
| title | In vitro surface efficacy of CaP-based anodised titanium for bone implants |
| title_full | In vitro surface efficacy of CaP-based anodised titanium for bone implants |
| title_fullStr | In vitro surface efficacy of CaP-based anodised titanium for bone implants |
| title_full_unstemmed | In vitro surface efficacy of CaP-based anodised titanium for bone implants |
| title_short | In vitro surface efficacy of CaP-based anodised titanium for bone implants |
| title_sort | in vitro surface efficacy of cap based anodised titanium for bone implants |
| topic | T Technology (General) |
| url | http://eprints.uthm.edu.my/11521/1/J16043_3ebf01813a855fc8bfbea2ea2e595eb8.pdf |
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