Visible and near-infrared spectroscopic determination of sugarcane chlorophyll content using a modified wavelength selection method for multivariate calibration
Spectroscopy in the visible and near-infrared region (Vis-NIR) region has proven to be an effective technique for quantifying the chlorophyll contents of plants, which serves as an important indicator of their photosynthetic rate and health status. However, the Vis-NIR spectroscopy analysis confro...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024
|
| Subjects: | |
| Online Access: | http://eprints.uthm.edu.my/11676/1/J16735_1feb4b8c010a4cf527ff4d0347961933.pdf |
| _version_ | 1825638587917402112 |
|---|---|
| author | Ong, Pauline Jian, Jinbao Li, Xiuhua Yin, Jianghua Ma, Guodong |
| author_facet | Ong, Pauline Jian, Jinbao Li, Xiuhua Yin, Jianghua Ma, Guodong |
| author_sort | Ong, Pauline |
| collection | UTHM |
| description | Spectroscopy in the visible and near-infrared region (Vis-NIR) region has proven to be an effective technique for
quantifying the chlorophyll contents of plants, which serves as an important indicator of their photosynthetic
rate and health status. However, the Vis-NIR spectroscopy analysis confronts a significant challenge concerning
the existence of spectral variations and interferences induced by diverse factors. Hence, the selection of characteristic wavelengths plays a crucial role in Vis-NIR spectroscopy analysis. In this study, a novel wavelength selection approach known as the modified regression coefficient (MRC) selection method was introduced to enhance the diagnostic accuracy of chlorophyll content in sugarcane leaves. Experimental data comprising spectral reflectance measurements (220–1400 nm) were collected from sugarcane leaf samples at different
growth stages, including seedling, tillering, and jointing, and the corresponding chlorophyll contents were measured. The proposed MRC method was employed to select optimal wavelengths for analysis, and subsequent partial least squares regression (PLSR) and Gaussian process regression (GPR) models were developed to establish the relationship between the selected wavelengths and the measured chlorophyll contents. In comparison to full-spectrum modelling and other commonly employed wavelength selection techniques, the proposed simplified MRC-GPR model, utilizing a subset of 291 selected wavelengths, demonstrated superior
performance. The MRC-GPR model achieved higher coefficient of determination of 0.9665 and 0.8659, and
lower root mean squared error of 1.7624 and 3.2029, for calibration set and prediction set, respectively. Results
showed that the GPR model, a nonlinear regression approach, outperformed the PLSR model. |
| first_indexed | 2024-12-08T07:42:34Z |
| format | Article |
| id | uthm.eprints-11676 |
| institution | Universiti Tun Hussein Onn Malaysia |
| language | English |
| last_indexed | 2024-12-08T07:42:34Z |
| publishDate | 2024 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | uthm.eprints-116762024-11-14T00:00:49Z http://eprints.uthm.edu.my/11676/ Visible and near-infrared spectroscopic determination of sugarcane chlorophyll content using a modified wavelength selection method for multivariate calibration Ong, Pauline Jian, Jinbao Li, Xiuhua Yin, Jianghua Ma, Guodong SB183 -317 Field crops Including cereals, forage crops, grasses, legumes, root crops, sugar plants, textile plants, alkaloidal plants, medicinal plants Spectroscopy in the visible and near-infrared region (Vis-NIR) region has proven to be an effective technique for quantifying the chlorophyll contents of plants, which serves as an important indicator of their photosynthetic rate and health status. However, the Vis-NIR spectroscopy analysis confronts a significant challenge concerning the existence of spectral variations and interferences induced by diverse factors. Hence, the selection of characteristic wavelengths plays a crucial role in Vis-NIR spectroscopy analysis. In this study, a novel wavelength selection approach known as the modified regression coefficient (MRC) selection method was introduced to enhance the diagnostic accuracy of chlorophyll content in sugarcane leaves. Experimental data comprising spectral reflectance measurements (220–1400 nm) were collected from sugarcane leaf samples at different growth stages, including seedling, tillering, and jointing, and the corresponding chlorophyll contents were measured. The proposed MRC method was employed to select optimal wavelengths for analysis, and subsequent partial least squares regression (PLSR) and Gaussian process regression (GPR) models were developed to establish the relationship between the selected wavelengths and the measured chlorophyll contents. In comparison to full-spectrum modelling and other commonly employed wavelength selection techniques, the proposed simplified MRC-GPR model, utilizing a subset of 291 selected wavelengths, demonstrated superior performance. The MRC-GPR model achieved higher coefficient of determination of 0.9665 and 0.8659, and lower root mean squared error of 1.7624 and 3.2029, for calibration set and prediction set, respectively. Results showed that the GPR model, a nonlinear regression approach, outperformed the PLSR model. Elsevier 2024 Article PeerReviewed text en http://eprints.uthm.edu.my/11676/1/J16735_1feb4b8c010a4cf527ff4d0347961933.pdf Ong, Pauline and Jian, Jinbao and Li, Xiuhua and Yin, Jianghua and Ma, Guodong (2024) Visible and near-infrared spectroscopic determination of sugarcane chlorophyll content using a modified wavelength selection method for multivariate calibration. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 305. pp. 1-15. https://doi.org/10.1016/j.saa.2023.123477 |
| spellingShingle | SB183 -317 Field crops Including cereals, forage crops, grasses, legumes, root crops, sugar plants, textile plants, alkaloidal plants, medicinal plants Ong, Pauline Jian, Jinbao Li, Xiuhua Yin, Jianghua Ma, Guodong Visible and near-infrared spectroscopic determination of sugarcane chlorophyll content using a modified wavelength selection method for multivariate calibration |
| title | Visible and near-infrared spectroscopic determination of sugarcane chlorophyll content using a modified wavelength selection method for multivariate calibration |
| title_full | Visible and near-infrared spectroscopic determination of sugarcane chlorophyll content using a modified wavelength selection method for multivariate calibration |
| title_fullStr | Visible and near-infrared spectroscopic determination of sugarcane chlorophyll content using a modified wavelength selection method for multivariate calibration |
| title_full_unstemmed | Visible and near-infrared spectroscopic determination of sugarcane chlorophyll content using a modified wavelength selection method for multivariate calibration |
| title_short | Visible and near-infrared spectroscopic determination of sugarcane chlorophyll content using a modified wavelength selection method for multivariate calibration |
| title_sort | visible and near infrared spectroscopic determination of sugarcane chlorophyll content using a modified wavelength selection method for multivariate calibration |
| topic | SB183 -317 Field crops Including cereals, forage crops, grasses, legumes, root crops, sugar plants, textile plants, alkaloidal plants, medicinal plants |
| url | http://eprints.uthm.edu.my/11676/1/J16735_1feb4b8c010a4cf527ff4d0347961933.pdf |
| work_keys_str_mv | AT ongpauline visibleandnearinfraredspectroscopicdeterminationofsugarcanechlorophyllcontentusingamodifiedwavelengthselectionmethodformultivariatecalibration AT jianjinbao visibleandnearinfraredspectroscopicdeterminationofsugarcanechlorophyllcontentusingamodifiedwavelengthselectionmethodformultivariatecalibration AT lixiuhua visibleandnearinfraredspectroscopicdeterminationofsugarcanechlorophyllcontentusingamodifiedwavelengthselectionmethodformultivariatecalibration AT yinjianghua visibleandnearinfraredspectroscopicdeterminationofsugarcanechlorophyllcontentusingamodifiedwavelengthselectionmethodformultivariatecalibration AT maguodong visibleandnearinfraredspectroscopicdeterminationofsugarcanechlorophyllcontentusingamodifiedwavelengthselectionmethodformultivariatecalibration |