Antioxidant, α-glucosidase inhibitory, and cytotoxic activities of Mangifera rufocostata extract and identification of its compounds by LC-MS/ MS analysis
Mangifera rufocostata, a member of the Mangifera genus, is a source of secondary metabolites and has been used traditionally as antidiabetic agent. Therefore, this study aims to determine the total flavonoid contens (TFC), total phenolic contents (TPC), antioxidant, α-glucosidase inhibitory, and cy...
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| Format: | Article |
| Language: | English |
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2024
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| Online Access: | http://eprints.uthm.edu.my/11717/1/J16902_cdb733027701d91024b2e466f832c5ac.pdf |
| _version_ | 1825638596116217856 |
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| author | Mustikasari, Kamilia Santoso, Mardi Abu Bakar, Mohd Fadzelly Sri Fatmawati, Sri Fatmawati |
| author_facet | Mustikasari, Kamilia Santoso, Mardi Abu Bakar, Mohd Fadzelly Sri Fatmawati, Sri Fatmawati |
| author_sort | Mustikasari, Kamilia |
| collection | UTHM |
| description | Mangifera rufocostata, a member of the Mangifera genus, is a source of secondary metabolites and has been used
traditionally as antidiabetic agent. Therefore, this study aims to determine the total flavonoid contens (TFC), total phenolic contents (TPC), antioxidant, α-glucosidase inhibitory, and cytotoxic activities of M. rufocostata stem bark extract. Antioxidant activity were evaluated by 2-2″-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and ferric reducing-antioxidant power (FRAP) tests. α-glucosidase inhibitory to prediction antidiabetic activity, and cytotoxic activity were studied against HT-29, HeLa, and MCF-7 cancer cell lines. The results showed that the methanol extract have the highest TFC, TPC, antioxidant, and α-glucosidase inhibitory activity. Furthermore, the methanol extract has strong cytotoxicity againts HT-29 cancer cells. Based on LC-MS/MS, the methanol extract contain several major compounds such as mangiferin, quinic acid, gallic acid, and 3-methoxy-4-hydroxy-phenyl glycol. |
| first_indexed | 2024-12-08T07:42:42Z |
| format | Article |
| id | uthm.eprints-11717 |
| institution | Universiti Tun Hussein Onn Malaysia |
| language | English |
| last_indexed | 2024-12-08T07:42:42Z |
| publishDate | 2024 |
| record_format | dspace |
| spelling | uthm.eprints-117172024-11-27T06:51:35Z http://eprints.uthm.edu.my/11717/ Antioxidant, α-glucosidase inhibitory, and cytotoxic activities of Mangifera rufocostata extract and identification of its compounds by LC-MS/ MS analysis Mustikasari, Kamilia Santoso, Mardi Abu Bakar, Mohd Fadzelly Sri Fatmawati, Sri Fatmawati RS153-441 Materia medica Mangifera rufocostata, a member of the Mangifera genus, is a source of secondary metabolites and has been used traditionally as antidiabetic agent. Therefore, this study aims to determine the total flavonoid contens (TFC), total phenolic contents (TPC), antioxidant, α-glucosidase inhibitory, and cytotoxic activities of M. rufocostata stem bark extract. Antioxidant activity were evaluated by 2-2″-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and ferric reducing-antioxidant power (FRAP) tests. α-glucosidase inhibitory to prediction antidiabetic activity, and cytotoxic activity were studied against HT-29, HeLa, and MCF-7 cancer cell lines. The results showed that the methanol extract have the highest TFC, TPC, antioxidant, and α-glucosidase inhibitory activity. Furthermore, the methanol extract has strong cytotoxicity againts HT-29 cancer cells. Based on LC-MS/MS, the methanol extract contain several major compounds such as mangiferin, quinic acid, gallic acid, and 3-methoxy-4-hydroxy-phenyl glycol. 2024 Article PeerReviewed text en http://eprints.uthm.edu.my/11717/1/J16902_cdb733027701d91024b2e466f832c5ac.pdf Mustikasari, Kamilia and Santoso, Mardi and Abu Bakar, Mohd Fadzelly and Sri Fatmawati, Sri Fatmawati (2024) Antioxidant, α-glucosidase inhibitory, and cytotoxic activities of Mangifera rufocostata extract and identification of its compounds by LC-MS/ MS analysis. Arabian Journal of Chemistry, 17. https://doi.org/10.1016/j.arabjc.2023.105391 |
| spellingShingle | RS153-441 Materia medica Mustikasari, Kamilia Santoso, Mardi Abu Bakar, Mohd Fadzelly Sri Fatmawati, Sri Fatmawati Antioxidant, α-glucosidase inhibitory, and cytotoxic activities of Mangifera rufocostata extract and identification of its compounds by LC-MS/ MS analysis |
| title | Antioxidant, α-glucosidase inhibitory, and cytotoxic activities of Mangifera rufocostata extract and identification of its compounds by LC-MS/ MS analysis |
| title_full | Antioxidant, α-glucosidase inhibitory, and cytotoxic activities of Mangifera rufocostata extract and identification of its compounds by LC-MS/ MS analysis |
| title_fullStr | Antioxidant, α-glucosidase inhibitory, and cytotoxic activities of Mangifera rufocostata extract and identification of its compounds by LC-MS/ MS analysis |
| title_full_unstemmed | Antioxidant, α-glucosidase inhibitory, and cytotoxic activities of Mangifera rufocostata extract and identification of its compounds by LC-MS/ MS analysis |
| title_short | Antioxidant, α-glucosidase inhibitory, and cytotoxic activities of Mangifera rufocostata extract and identification of its compounds by LC-MS/ MS analysis |
| title_sort | antioxidant α glucosidase inhibitory and cytotoxic activities of mangifera rufocostata extract and identification of its compounds by lc ms ms analysis |
| topic | RS153-441 Materia medica |
| url | http://eprints.uthm.edu.my/11717/1/J16902_cdb733027701d91024b2e466f832c5ac.pdf |
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