| Summary: | In this study, we propose the manipulation and
cell injection of a fluorescent microsensor using multiple
wavelengths of light. The fluorescent microsensor is made of a
1-μm polystyrene particle containing infrared (IR: 808 nm)
absorbing dye and Rhodamine B. The polystyrene particle can
be manipulated in water using a 1064-nm laser because the
refractive index of the polystyrene is 1.6 (refractive index of
water: 1.3). The IR absorbing dye absorbs 808-nm light but does
not absorb the 1064-nm laser. Rhodamine B is a
temperature-sensitive fluorescent dye (excitation wavelength:
488 nm, emission wavelength: 560 nm). The functions of
manipulation, heating for injection, and temperature
measurement are achieved by different wavelengths of 1064 nm,
808 nm, and 488 nm, respectively. The temperature increase of
fluorescent microsensor with 808-nm (40 mW, 10 s) laser was
approximately 15°C, and enough for injection of fluorescent
microsensor. We demonstrated manipulation and injection of
the microsensor into Madin-Darby canine kidney cell using
1064-nm and 808-nm lasers. These results confirmed the
effectiveness of our proposed cell injection of a fluorescent
microsensor using multiple wavelengths of light.
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