Injection of a fluorescent microsensor into a specific cell by laser manipulation and heating with multiple wavelengths of light
In this study, we propose the manipulation and cell injection of a fluorescent microsensor using multiple wavelengths of light. The fluorescent microsensor is made of a 1-μm polystyrene particle containing infrared (IR: 808 nm) absorbing dye and Rhodamine B. The polystyrene particle can be mani...
| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2021
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| Subjects: | |
| Online Access: | http://eprints.uthm.edu.my/1871/1/P12587_a1a5482b9f69a259be5e6bb0325f7c97.pdf |
| _version_ | 1796868541005168640 |
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| author | Maruyama, Hisataka Hashim, Hairulazwan Yanagawa, Ryota Arai, Fumihito |
| author_facet | Maruyama, Hisataka Hashim, Hairulazwan Yanagawa, Ryota Arai, Fumihito |
| author_sort | Maruyama, Hisataka |
| collection | UTHM |
| description | In this study, we propose the manipulation and
cell injection of a fluorescent microsensor using multiple
wavelengths of light. The fluorescent microsensor is made of a
1-μm polystyrene particle containing infrared (IR: 808 nm)
absorbing dye and Rhodamine B. The polystyrene particle can
be manipulated in water using a 1064-nm laser because the
refractive index of the polystyrene is 1.6 (refractive index of
water: 1.3). The IR absorbing dye absorbs 808-nm light but does
not absorb the 1064-nm laser. Rhodamine B is a
temperature-sensitive fluorescent dye (excitation wavelength:
488 nm, emission wavelength: 560 nm). The functions of
manipulation, heating for injection, and temperature
measurement are achieved by different wavelengths of 1064 nm,
808 nm, and 488 nm, respectively. The temperature increase of
fluorescent microsensor with 808-nm (40 mW, 10 s) laser was
approximately 15°C, and enough for injection of fluorescent
microsensor. We demonstrated manipulation and injection of
the microsensor into Madin-Darby canine kidney cell using
1064-nm and 808-nm lasers. These results confirmed the
effectiveness of our proposed cell injection of a fluorescent
microsensor using multiple wavelengths of light. |
| first_indexed | 2024-03-05T21:41:19Z |
| format | Conference or Workshop Item |
| id | uthm.eprints-1871 |
| institution | Universiti Tun Hussein Onn Malaysia |
| language | English |
| last_indexed | 2024-03-05T21:41:19Z |
| publishDate | 2021 |
| record_format | dspace |
| spelling | uthm.eprints-18712021-10-20T07:23:17Z http://eprints.uthm.edu.my/1871/ Injection of a fluorescent microsensor into a specific cell by laser manipulation and heating with multiple wavelengths of light Maruyama, Hisataka Hashim, Hairulazwan Yanagawa, Ryota Arai, Fumihito TA1501-1820 Applied optics. Photonics In this study, we propose the manipulation and cell injection of a fluorescent microsensor using multiple wavelengths of light. The fluorescent microsensor is made of a 1-μm polystyrene particle containing infrared (IR: 808 nm) absorbing dye and Rhodamine B. The polystyrene particle can be manipulated in water using a 1064-nm laser because the refractive index of the polystyrene is 1.6 (refractive index of water: 1.3). The IR absorbing dye absorbs 808-nm light but does not absorb the 1064-nm laser. Rhodamine B is a temperature-sensitive fluorescent dye (excitation wavelength: 488 nm, emission wavelength: 560 nm). The functions of manipulation, heating for injection, and temperature measurement are achieved by different wavelengths of 1064 nm, 808 nm, and 488 nm, respectively. The temperature increase of fluorescent microsensor with 808-nm (40 mW, 10 s) laser was approximately 15°C, and enough for injection of fluorescent microsensor. We demonstrated manipulation and injection of the microsensor into Madin-Darby canine kidney cell using 1064-nm and 808-nm lasers. These results confirmed the effectiveness of our proposed cell injection of a fluorescent microsensor using multiple wavelengths of light. 2021 Conference or Workshop Item PeerReviewed text en http://eprints.uthm.edu.my/1871/1/P12587_a1a5482b9f69a259be5e6bb0325f7c97.pdf Maruyama, Hisataka and Hashim, Hairulazwan and Yanagawa, Ryota and Arai, Fumihito (2021) Injection of a fluorescent microsensor into a specific cell by laser manipulation and heating with multiple wavelengths of light. In: 2020 IEEE International Conference on Robotics and Automation (ICRA), 31 May - 31 August, 2020, Paris, France. |
| spellingShingle | TA1501-1820 Applied optics. Photonics Maruyama, Hisataka Hashim, Hairulazwan Yanagawa, Ryota Arai, Fumihito Injection of a fluorescent microsensor into a specific cell by laser manipulation and heating with multiple wavelengths of light |
| title | Injection of a fluorescent microsensor into a specific cell by
laser manipulation and heating with multiple wavelengths of light |
| title_full | Injection of a fluorescent microsensor into a specific cell by
laser manipulation and heating with multiple wavelengths of light |
| title_fullStr | Injection of a fluorescent microsensor into a specific cell by
laser manipulation and heating with multiple wavelengths of light |
| title_full_unstemmed | Injection of a fluorescent microsensor into a specific cell by
laser manipulation and heating with multiple wavelengths of light |
| title_short | Injection of a fluorescent microsensor into a specific cell by
laser manipulation and heating with multiple wavelengths of light |
| title_sort | injection of a fluorescent microsensor into a specific cell by laser manipulation and heating with multiple wavelengths of light |
| topic | TA1501-1820 Applied optics. Photonics |
| url | http://eprints.uthm.edu.my/1871/1/P12587_a1a5482b9f69a259be5e6bb0325f7c97.pdf |
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