A review on environmental DNA (eDNA) metabarcoding markers for wildlife monitoring research

Environmental DNA or eDNA utilizes traceable genetic materials in the environment for monitoring the presence of organisms in a given area and it is now gaining popularity as an alternative for traditional monitoring methods. Thus, the selection of genetic markers is crucial for identification of...

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Main Authors: Othman, N., Haris, H., Fatin, Z., Najmuddin, M. F., Sariyati, N. H., Md-Zain, B., Abdul-Latiff, M. A.
Format: Conference or Workshop Item
Language:English
Published: 2021
Subjects:
Online Access:http://eprints.uthm.edu.my/2568/1/P12637_eee570742e93d9bac6e3a71aee0954df.pdf
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author Othman, N.
Haris, H.
Fatin, Z.
Najmuddin, M. F.
Sariyati, N. H.
Md-Zain, B.
Abdul-Latiff, M. A.
author_facet Othman, N.
Haris, H.
Fatin, Z.
Najmuddin, M. F.
Sariyati, N. H.
Md-Zain, B.
Abdul-Latiff, M. A.
author_sort Othman, N.
collection UTHM
description Environmental DNA or eDNA utilizes traceable genetic materials in the environment for monitoring the presence of organisms in a given area and it is now gaining popularity as an alternative for traditional monitoring methods. Thus, the selection of genetic markers is crucial for identification of species in wildlife monitoring. This paper aims to review several DNA markers which are appropriate and reliable for detection of organisms from the environmental samples. We performed systematic literature search from SCOPUS database to review all molecular markers of eDNA. This study focuses on the importance of markers selection which can be utilized by next-generation sequencing (NGS) for biodiversity monitoring. Cytochrome C oxidase Subunit I (COI) are noted as the most widely used marker in metabarcoding research for detection of targeted species.
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spelling uthm.eprints-25682021-10-25T04:10:31Z http://eprints.uthm.edu.my/2568/ A review on environmental DNA (eDNA) metabarcoding markers for wildlife monitoring research Othman, N. Haris, H. Fatin, Z. Najmuddin, M. F. Sariyati, N. H. Md-Zain, B. Abdul-Latiff, M. A. SD411 -428 Conservation and protection Including forest influences, damage by elements, fires, forest reserves T Technology (General) Environmental DNA or eDNA utilizes traceable genetic materials in the environment for monitoring the presence of organisms in a given area and it is now gaining popularity as an alternative for traditional monitoring methods. Thus, the selection of genetic markers is crucial for identification of species in wildlife monitoring. This paper aims to review several DNA markers which are appropriate and reliable for detection of organisms from the environmental samples. We performed systematic literature search from SCOPUS database to review all molecular markers of eDNA. This study focuses on the importance of markers selection which can be utilized by next-generation sequencing (NGS) for biodiversity monitoring. Cytochrome C oxidase Subunit I (COI) are noted as the most widely used marker in metabarcoding research for detection of targeted species. 2021 Conference or Workshop Item PeerReviewed text en http://eprints.uthm.edu.my/2568/1/P12637_eee570742e93d9bac6e3a71aee0954df.pdf Othman, N. and Haris, H. and Fatin, Z. and Najmuddin, M. F. and Sariyati, N. H. and Md-Zain, B. and Abdul-Latiff, M. A. (2021) A review on environmental DNA (eDNA) metabarcoding markers for wildlife monitoring research. In: International Conference on Biodiversity 2020, November 4-5, 2020, Melaka, Malaysia. https://doi.org/:10.1088/1755-1315/736/1/012054
spellingShingle SD411 -428 Conservation and protection Including forest influences, damage by elements, fires, forest reserves
T Technology (General)
Othman, N.
Haris, H.
Fatin, Z.
Najmuddin, M. F.
Sariyati, N. H.
Md-Zain, B.
Abdul-Latiff, M. A.
A review on environmental DNA (eDNA) metabarcoding markers for wildlife monitoring research
title A review on environmental DNA (eDNA) metabarcoding markers for wildlife monitoring research
title_full A review on environmental DNA (eDNA) metabarcoding markers for wildlife monitoring research
title_fullStr A review on environmental DNA (eDNA) metabarcoding markers for wildlife monitoring research
title_full_unstemmed A review on environmental DNA (eDNA) metabarcoding markers for wildlife monitoring research
title_short A review on environmental DNA (eDNA) metabarcoding markers for wildlife monitoring research
title_sort review on environmental dna edna metabarcoding markers for wildlife monitoring research
topic SD411 -428 Conservation and protection Including forest influences, damage by elements, fires, forest reserves
T Technology (General)
url http://eprints.uthm.edu.my/2568/1/P12637_eee570742e93d9bac6e3a71aee0954df.pdf
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