Phylogenetic study of presumptive oil-degrading microbes isolated from the north-western tip of Pahang
Many construction areas are often contaminated with petroleum compounds. The aim of this work were to isolate and characterize indigenous bacteria isolated at a moderate temperature site as well as to study the pattern of phylogenetic tree among bacterial communities associated with oil degradation....
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| Format: | Article |
| Language: | English |
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Penerbit UTHM
2018
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| Online Access: | http://eprints.uthm.edu.my/3649/1/AJ%202018%20%28708%29%20Phylogenetic%20study%20of%20presumptive%20oil-degrading%20microbes%20isolated%20from%20the%20north-western%20tip%20of%20Pahang.pdf |
| _version_ | 1796868876524322816 |
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| author | Azizan, Nur Hafizah Md Rami, Siti Zakiah Saedudin, RD Rohmat Kasim, Shahreen |
| author_facet | Azizan, Nur Hafizah Md Rami, Siti Zakiah Saedudin, RD Rohmat Kasim, Shahreen |
| author_sort | Azizan, Nur Hafizah |
| collection | UTHM |
| description | Many construction areas are often contaminated with petroleum compounds. The aim of this work were to isolate and characterize indigenous bacteria isolated at a moderate temperature site as well as to study the pattern of phylogenetic tree among bacterial communities associated with oil degradation. No profound studies have yet been done in the construction site at Tanah Rata. Hence, this research was carried out to find existing status of microbial community from a few selected spots. Enrichment culture technique by using MSM broth has been used to isolate the desired microorganisms. Isolation and characterization tests using phenotypic and genotypic approaches (based on genes encoding 16S rRNA) had led to the discovery of 18 isolates. The 16S rRNA was used due to its functional constant, universally distributed and moderately well discovered across broad phylogenetic distances. The successfully identified genera were Pseudomonas, Bacillus, Exiguobacterium, Stenotrophomonas, Acinetobacter, Serratia and Gamma Proteobacterium. |
| first_indexed | 2024-03-05T21:46:24Z |
| format | Article |
| id | uthm.eprints-3649 |
| institution | Universiti Tun Hussein Onn Malaysia |
| language | English |
| last_indexed | 2024-03-05T21:46:24Z |
| publishDate | 2018 |
| publisher | Penerbit UTHM |
| record_format | dspace |
| spelling | uthm.eprints-36492021-11-21T06:26:42Z http://eprints.uthm.edu.my/3649/ Phylogenetic study of presumptive oil-degrading microbes isolated from the north-western tip of Pahang Azizan, Nur Hafizah Md Rami, Siti Zakiah Saedudin, RD Rohmat Kasim, Shahreen TP690-692.5 Petroleum refining. Petroleum products Many construction areas are often contaminated with petroleum compounds. The aim of this work were to isolate and characterize indigenous bacteria isolated at a moderate temperature site as well as to study the pattern of phylogenetic tree among bacterial communities associated with oil degradation. No profound studies have yet been done in the construction site at Tanah Rata. Hence, this research was carried out to find existing status of microbial community from a few selected spots. Enrichment culture technique by using MSM broth has been used to isolate the desired microorganisms. Isolation and characterization tests using phenotypic and genotypic approaches (based on genes encoding 16S rRNA) had led to the discovery of 18 isolates. The 16S rRNA was used due to its functional constant, universally distributed and moderately well discovered across broad phylogenetic distances. The successfully identified genera were Pseudomonas, Bacillus, Exiguobacterium, Stenotrophomonas, Acinetobacter, Serratia and Gamma Proteobacterium. Penerbit UTHM 2018 Article PeerReviewed text en http://eprints.uthm.edu.my/3649/1/AJ%202018%20%28708%29%20Phylogenetic%20study%20of%20presumptive%20oil-degrading%20microbes%20isolated%20from%20the%20north-western%20tip%20of%20Pahang.pdf Azizan, Nur Hafizah and Md Rami, Siti Zakiah and Saedudin, RD Rohmat and Kasim, Shahreen (2018) Phylogenetic study of presumptive oil-degrading microbes isolated from the north-western tip of Pahang. International Journal of Integrated Engineering, 10 (6). pp. 128-132. ISSN 2229-838X |
| spellingShingle | TP690-692.5 Petroleum refining. Petroleum products Azizan, Nur Hafizah Md Rami, Siti Zakiah Saedudin, RD Rohmat Kasim, Shahreen Phylogenetic study of presumptive oil-degrading microbes isolated from the north-western tip of Pahang |
| title | Phylogenetic study of presumptive oil-degrading microbes isolated from the north-western tip of Pahang |
| title_full | Phylogenetic study of presumptive oil-degrading microbes isolated from the north-western tip of Pahang |
| title_fullStr | Phylogenetic study of presumptive oil-degrading microbes isolated from the north-western tip of Pahang |
| title_full_unstemmed | Phylogenetic study of presumptive oil-degrading microbes isolated from the north-western tip of Pahang |
| title_short | Phylogenetic study of presumptive oil-degrading microbes isolated from the north-western tip of Pahang |
| title_sort | phylogenetic study of presumptive oil degrading microbes isolated from the north western tip of pahang |
| topic | TP690-692.5 Petroleum refining. Petroleum products |
| url | http://eprints.uthm.edu.my/3649/1/AJ%202018%20%28708%29%20Phylogenetic%20study%20of%20presumptive%20oil-degrading%20microbes%20isolated%20from%20the%20north-western%20tip%20of%20Pahang.pdf |
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