Isolation and Characterization of Hydrocarbon Tolerant Microorganisms from Marine Environment

Industrial activities have contributed to the releases of toxic organic compound into environment and have become a major public concern. This particularly of those industries that are located along coastal areas, which are the gateways for water transport. A study of the isolation and characterizat...

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Main Authors: Azizan, Nur Hafizah, Mahamad Kisim, Nur Fairuz, Saedudin, RD Rohmat, Zainal Abidin, Zaima Azira, Mohd Ashaari, Mardiana, Kasim, Shahreen
Format: Article
Language:English
Published: Universiti Tun Hussein Onn Malaysia (UTHM) 2018
Subjects:
Online Access:http://eprints.uthm.edu.my/3650/1/AJ%202018%20%28709%29%20Isolation%20and%20Characterization%20of%20Hydrocarbon%20Tolerant%20Microorganisms%20from%20Marine%20Environment.pdf
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author Azizan, Nur Hafizah
Mahamad Kisim, Nur Fairuz
Saedudin, RD Rohmat
Zainal Abidin, Zaima Azira
Mohd Ashaari, Mardiana
Kasim, Shahreen
author_facet Azizan, Nur Hafizah
Mahamad Kisim, Nur Fairuz
Saedudin, RD Rohmat
Zainal Abidin, Zaima Azira
Mohd Ashaari, Mardiana
Kasim, Shahreen
author_sort Azizan, Nur Hafizah
collection UTHM
description Industrial activities have contributed to the releases of toxic organic compound into environment and have become a major public concern. This particularly of those industries that are located along coastal areas, which are the gateways for water transport. A study of the isolation and characterization of hydrocarbon tolerant microorganisms from marine samples collected at the jetty site of Tanjung Lumpur, Kuantan, Pahang, was conducted. There were very few studies have been done related to marine hydrocarbon tolerant microorganisms in Kuantan. Hence, this research was done to investigate the presence of microbial community that can thrive in the environment with oil-spillage. Enrichment culture technique by using MSM broth supplemented with 1% engine oil was utilized to isolate the desired microorganisms. Biochemical and molecular approaches were applied to identify and characterize the isolates. Six isolates wereidentified as genera Vibrio, Halomonas, Pseudoaltromonas, Idiomarina, Staphylococcus and Halophilic bacterium. In addition, phylogenetic study helps further in understand the relationship among the isolated bacteria.
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spelling uthm.eprints-36502021-11-21T06:27:07Z http://eprints.uthm.edu.my/3650/ Isolation and Characterization of Hydrocarbon Tolerant Microorganisms from Marine Environment Azizan, Nur Hafizah Mahamad Kisim, Nur Fairuz Saedudin, RD Rohmat Zainal Abidin, Zaima Azira Mohd Ashaari, Mardiana Kasim, Shahreen TC183-201 General preliminary operations. Dredging. Submarine building Industrial activities have contributed to the releases of toxic organic compound into environment and have become a major public concern. This particularly of those industries that are located along coastal areas, which are the gateways for water transport. A study of the isolation and characterization of hydrocarbon tolerant microorganisms from marine samples collected at the jetty site of Tanjung Lumpur, Kuantan, Pahang, was conducted. There were very few studies have been done related to marine hydrocarbon tolerant microorganisms in Kuantan. Hence, this research was done to investigate the presence of microbial community that can thrive in the environment with oil-spillage. Enrichment culture technique by using MSM broth supplemented with 1% engine oil was utilized to isolate the desired microorganisms. Biochemical and molecular approaches were applied to identify and characterize the isolates. Six isolates wereidentified as genera Vibrio, Halomonas, Pseudoaltromonas, Idiomarina, Staphylococcus and Halophilic bacterium. In addition, phylogenetic study helps further in understand the relationship among the isolated bacteria. Universiti Tun Hussein Onn Malaysia (UTHM) 2018 Article PeerReviewed text en http://eprints.uthm.edu.my/3650/1/AJ%202018%20%28709%29%20Isolation%20and%20Characterization%20of%20Hydrocarbon%20Tolerant%20Microorganisms%20from%20Marine%20Environment.pdf Azizan, Nur Hafizah and Mahamad Kisim, Nur Fairuz and Saedudin, RD Rohmat and Zainal Abidin, Zaima Azira and Mohd Ashaari, Mardiana and Kasim, Shahreen (2018) Isolation and Characterization of Hydrocarbon Tolerant Microorganisms from Marine Environment. International Journal of Integrated Engineering (IJIE), 10 (6). pp. 133-138. ISSN 2229-838X
spellingShingle TC183-201 General preliminary operations. Dredging. Submarine building
Azizan, Nur Hafizah
Mahamad Kisim, Nur Fairuz
Saedudin, RD Rohmat
Zainal Abidin, Zaima Azira
Mohd Ashaari, Mardiana
Kasim, Shahreen
Isolation and Characterization of Hydrocarbon Tolerant Microorganisms from Marine Environment
title Isolation and Characterization of Hydrocarbon Tolerant Microorganisms from Marine Environment
title_full Isolation and Characterization of Hydrocarbon Tolerant Microorganisms from Marine Environment
title_fullStr Isolation and Characterization of Hydrocarbon Tolerant Microorganisms from Marine Environment
title_full_unstemmed Isolation and Characterization of Hydrocarbon Tolerant Microorganisms from Marine Environment
title_short Isolation and Characterization of Hydrocarbon Tolerant Microorganisms from Marine Environment
title_sort isolation and characterization of hydrocarbon tolerant microorganisms from marine environment
topic TC183-201 General preliminary operations. Dredging. Submarine building
url http://eprints.uthm.edu.my/3650/1/AJ%202018%20%28709%29%20Isolation%20and%20Characterization%20of%20Hydrocarbon%20Tolerant%20Microorganisms%20from%20Marine%20Environment.pdf
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AT saedudinrdrohmat isolationandcharacterizationofhydrocarbontolerantmicroorganismsfrommarineenvironment
AT zainalabidinzaimaazira isolationandcharacterizationofhydrocarbontolerantmicroorganismsfrommarineenvironment
AT mohdashaarimardiana isolationandcharacterizationofhydrocarbontolerantmicroorganismsfrommarineenvironment
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