In vitro growth of human keratinocytes and oral cancer cells into microtissues: an aerosol-based microencapsulation technique
Cells encapsulation is a micro-technology widely applied in cell and tissue research, tissue transplantation, and regenerative medicine. In this paper, we proposed a growth of microtissue model for the human keratinocytes (HaCaT) cell line and an oral squamous cell carcinoma (OSCC) cell line (ORL-48...
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| Format: | Article |
| Language: | English |
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Multidisciplinary Digital Publishing Institute
2017
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| Online Access: | http://eprints.uthm.edu.my/3700/1/AJ%202017%20%2890%29%20In%20vitro%20growth%20of%20human%20keratinocytes.pdf |
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| author | Leong, Wai Yean Soon, Chin Fhong Wong, Soon Chuan Tee, Kian Sek Cheong, Sok Ching Gan, Siew Hua Youseffi, Mansour |
| author_facet | Leong, Wai Yean Soon, Chin Fhong Wong, Soon Chuan Tee, Kian Sek Cheong, Sok Ching Gan, Siew Hua Youseffi, Mansour |
| author_sort | Leong, Wai Yean |
| collection | UTHM |
| description | Cells encapsulation is a micro-technology widely applied in cell and tissue research, tissue transplantation, and regenerative medicine. In this paper, we proposed a growth of microtissue model for the human keratinocytes (HaCaT) cell line and an oral squamous cell carcinoma (OSCC) cell line (ORL-48) based on a simple aerosol microencapsulation technique. At an extrusion rate of 20 µL/min and air flow rate of 0.3 L/min programmed in the aerosol system, HaCaT and ORL-48 cells in alginate microcapsules were encapsulated in microcapsules with a diameter ranging from 200 to 300 µm. Both cell lines were successfully grown into microtissues in the microcapsules of alginate within 16 days of culture. The microtissues were characterized by using a live/dead cell viability assay, field emission-scanning electron microscopy (FE-SEM), fluorescence staining, and cell re-plating experiments. The microtissues of both cell types were viable after being extracted from the alginate membrane using alginate lyase. However, the microtissues of HaCaT and ORL-48 demonstrated differences in both nucleus size and morphology. The microtissues with re-associated cells in spheroids are potentially useful as a cell model for pharmacological studies. |
| first_indexed | 2024-03-05T21:46:33Z |
| format | Article |
| id | uthm.eprints-3700 |
| institution | Universiti Tun Hussein Onn Malaysia |
| language | English |
| last_indexed | 2024-03-05T21:46:33Z |
| publishDate | 2017 |
| publisher | Multidisciplinary Digital Publishing Institute |
| record_format | dspace |
| spelling | uthm.eprints-37002021-11-21T07:50:44Z http://eprints.uthm.edu.my/3700/ In vitro growth of human keratinocytes and oral cancer cells into microtissues: an aerosol-based microencapsulation technique Leong, Wai Yean Soon, Chin Fhong Wong, Soon Chuan Tee, Kian Sek Cheong, Sok Ching Gan, Siew Hua Youseffi, Mansour QD Chemistry Cells encapsulation is a micro-technology widely applied in cell and tissue research, tissue transplantation, and regenerative medicine. In this paper, we proposed a growth of microtissue model for the human keratinocytes (HaCaT) cell line and an oral squamous cell carcinoma (OSCC) cell line (ORL-48) based on a simple aerosol microencapsulation technique. At an extrusion rate of 20 µL/min and air flow rate of 0.3 L/min programmed in the aerosol system, HaCaT and ORL-48 cells in alginate microcapsules were encapsulated in microcapsules with a diameter ranging from 200 to 300 µm. Both cell lines were successfully grown into microtissues in the microcapsules of alginate within 16 days of culture. The microtissues were characterized by using a live/dead cell viability assay, field emission-scanning electron microscopy (FE-SEM), fluorescence staining, and cell re-plating experiments. The microtissues of both cell types were viable after being extracted from the alginate membrane using alginate lyase. However, the microtissues of HaCaT and ORL-48 demonstrated differences in both nucleus size and morphology. The microtissues with re-associated cells in spheroids are potentially useful as a cell model for pharmacological studies. Multidisciplinary Digital Publishing Institute 2017 Article PeerReviewed text en http://eprints.uthm.edu.my/3700/1/AJ%202017%20%2890%29%20In%20vitro%20growth%20of%20human%20keratinocytes.pdf Leong, Wai Yean and Soon, Chin Fhong and Wong, Soon Chuan and Tee, Kian Sek and Cheong, Sok Ching and Gan, Siew Hua and Youseffi, Mansour (2017) In vitro growth of human keratinocytes and oral cancer cells into microtissues: an aerosol-based microencapsulation technique. Bioengineering, 4 (45). pp. 1-14. ISSN 2306-5354 http://dx.doi.org/10.3390/bioengineering4020043 |
| spellingShingle | QD Chemistry Leong, Wai Yean Soon, Chin Fhong Wong, Soon Chuan Tee, Kian Sek Cheong, Sok Ching Gan, Siew Hua Youseffi, Mansour In vitro growth of human keratinocytes and oral cancer cells into microtissues: an aerosol-based microencapsulation technique |
| title | In vitro growth of human keratinocytes and oral cancer cells into microtissues: an aerosol-based microencapsulation technique |
| title_full | In vitro growth of human keratinocytes and oral cancer cells into microtissues: an aerosol-based microencapsulation technique |
| title_fullStr | In vitro growth of human keratinocytes and oral cancer cells into microtissues: an aerosol-based microencapsulation technique |
| title_full_unstemmed | In vitro growth of human keratinocytes and oral cancer cells into microtissues: an aerosol-based microencapsulation technique |
| title_short | In vitro growth of human keratinocytes and oral cancer cells into microtissues: an aerosol-based microencapsulation technique |
| title_sort | in vitro growth of human keratinocytes and oral cancer cells into microtissues an aerosol based microencapsulation technique |
| topic | QD Chemistry |
| url | http://eprints.uthm.edu.my/3700/1/AJ%202017%20%2890%29%20In%20vitro%20growth%20of%20human%20keratinocytes.pdf |
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