Identification of bacteria associated with Holothuria (Mertensiothuria) leucospilota from Pangkor Island

Holothuria (Mertensiothuria) leucospilota is the most abundant sea cucumber species in Malaysia. This study aimed to identify bacteria isolated from the external and internal body parts of H. leucospilota collected from the coastal water of Pangkor Island, Perak, Malaysia. A total of 26 bacterial sa...

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Main Authors: Muhammad Lutfi, Fatin Najihah, Mohamed Rehan, Maryam, Kamarudin, Kamarul Rahim, Che Zahri, Mohd Zulhafiz, Mohd Noor, Hanina, Mat Radzi, Salina
Format: Article
Language:English
Published: Malaysian Society of Applied Biology 2018
Subjects:
Online Access:http://eprints.uthm.edu.my/5802/1/AJ%202018%20%28584%29.pdf
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author Muhammad Lutfi, Fatin Najihah
Mohamed Rehan, Maryam
Kamarudin, Kamarul Rahim
Che Zahri, Mohd Zulhafiz
Mohd Noor, Hanina
Mat Radzi, Salina
author_facet Muhammad Lutfi, Fatin Najihah
Mohamed Rehan, Maryam
Kamarudin, Kamarul Rahim
Che Zahri, Mohd Zulhafiz
Mohd Noor, Hanina
Mat Radzi, Salina
author_sort Muhammad Lutfi, Fatin Najihah
collection UTHM
description Holothuria (Mertensiothuria) leucospilota is the most abundant sea cucumber species in Malaysia. This study aimed to identify bacteria isolated from the external and internal body parts of H. leucospilota collected from the coastal water of Pangkor Island, Perak, Malaysia. A total of 26 bacterial samples were isolated using streak plate method from eight body parts of two fresh H. leucospilota specimens and from the surrounding surface sediments and seawater. Identification of the bacterial isolates was based on microscopic examination, 16S rDNA amplification and phylogenetic analysis using the neighbour-joining method. Three genera of bacteria were identified namely Vibrio, Bacillus, and Acinetobacter. The genus Vibrio was found to be the main bacterial group associated with the H. leucospilota specimens from Pangkor Island.
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spelling uthm.eprints-58022022-01-23T08:17:40Z http://eprints.uthm.edu.my/5802/ Identification of bacteria associated with Holothuria (Mertensiothuria) leucospilota from Pangkor Island Muhammad Lutfi, Fatin Najihah Mohamed Rehan, Maryam Kamarudin, Kamarul Rahim Che Zahri, Mohd Zulhafiz Mohd Noor, Hanina Mat Radzi, Salina TP Chemical technology Holothuria (Mertensiothuria) leucospilota is the most abundant sea cucumber species in Malaysia. This study aimed to identify bacteria isolated from the external and internal body parts of H. leucospilota collected from the coastal water of Pangkor Island, Perak, Malaysia. A total of 26 bacterial samples were isolated using streak plate method from eight body parts of two fresh H. leucospilota specimens and from the surrounding surface sediments and seawater. Identification of the bacterial isolates was based on microscopic examination, 16S rDNA amplification and phylogenetic analysis using the neighbour-joining method. Three genera of bacteria were identified namely Vibrio, Bacillus, and Acinetobacter. The genus Vibrio was found to be the main bacterial group associated with the H. leucospilota specimens from Pangkor Island. Malaysian Society of Applied Biology 2018 Article PeerReviewed text en http://eprints.uthm.edu.my/5802/1/AJ%202018%20%28584%29.pdf Muhammad Lutfi, Fatin Najihah and Mohamed Rehan, Maryam and Kamarudin, Kamarul Rahim and Che Zahri, Mohd Zulhafiz and Mohd Noor, Hanina and Mat Radzi, Salina (2018) Identification of bacteria associated with Holothuria (Mertensiothuria) leucospilota from Pangkor Island. Malaysian Applied Biology, 47 (2). pp. 95-101. ISSN 0126-8643
spellingShingle TP Chemical technology
Muhammad Lutfi, Fatin Najihah
Mohamed Rehan, Maryam
Kamarudin, Kamarul Rahim
Che Zahri, Mohd Zulhafiz
Mohd Noor, Hanina
Mat Radzi, Salina
Identification of bacteria associated with Holothuria (Mertensiothuria) leucospilota from Pangkor Island
title Identification of bacteria associated with Holothuria (Mertensiothuria) leucospilota from Pangkor Island
title_full Identification of bacteria associated with Holothuria (Mertensiothuria) leucospilota from Pangkor Island
title_fullStr Identification of bacteria associated with Holothuria (Mertensiothuria) leucospilota from Pangkor Island
title_full_unstemmed Identification of bacteria associated with Holothuria (Mertensiothuria) leucospilota from Pangkor Island
title_short Identification of bacteria associated with Holothuria (Mertensiothuria) leucospilota from Pangkor Island
title_sort identification of bacteria associated with holothuria mertensiothuria leucospilota from pangkor island
topic TP Chemical technology
url http://eprints.uthm.edu.my/5802/1/AJ%202018%20%28584%29.pdf
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