Characterisation of calcium carbonate formed by Bacillus Sphaericus via fermentation of urea

Microbiologically induced calcium carbonate precipitation (MICCP) has emerged as rather promising green technology compares to conventional calcium carbonate process. Bacillus sphaericus LMG22557 is a superior MICCP-capable microorganism that can potentially utilise urea from diverse sources. This s...

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Main Authors: Azmi, Muhammad Syazwan, Teng, Siaw Lin, Tajarudin, Husnul Azan, Makhtar, Muaz Mohd Zaini, Daud, Zawawi
Format: Article
Language:English
Published: UTHM Publisher 2018
Subjects:
Online Access:http://eprints.uthm.edu.my/5989/1/AJ%202017%20%28937%29.pdf
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author Azmi, Muhammad Syazwan
Teng, Siaw Lin
Tajarudin, Husnul Azan
Makhtar, Muaz Mohd Zaini
Daud, Zawawi
author_facet Azmi, Muhammad Syazwan
Teng, Siaw Lin
Tajarudin, Husnul Azan
Makhtar, Muaz Mohd Zaini
Daud, Zawawi
author_sort Azmi, Muhammad Syazwan
collection UTHM
description Microbiologically induced calcium carbonate precipitation (MICCP) has emerged as rather promising green technology compares to conventional calcium carbonate process. Bacillus sphaericus LMG22557 is a superior MICCP-capable microorganism that can potentially utilise urea from diverse sources. This study focuses on maximising and characterising the calcium carbonate produced from fermentation of synthetic urea and waste urea from chicken manure. Morphologies of calcium carbonate attributed from fermentation of urea and precipitation by different calcium source were studied by microscopic observation, Fourier-Transform Infrared (FTIR) and X-Ray Diffraction (XRD). Precipitation from calcium chloride produced more vaterite while precipitation from calcium nitrate tetrahydrate produced pure calcite. Higher specific yield productivity (0.2502 mol/g cell/h) was achieved with 200 rpm and 40 g/L urea concentration. The growth of B. sphaericus in chicken manure solution was achievable but with very low yield of bicarbonate ions (less than 0.0008 mol/g cells/h). In brief, B. sphaericus is able to produce calcium carbonate from synthetic urea while further study is required for chicken manure to be used as substitute.
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spelling uthm.eprints-59892022-01-26T01:38:30Z http://eprints.uthm.edu.my/5989/ Characterisation of calcium carbonate formed by Bacillus Sphaericus via fermentation of urea Azmi, Muhammad Syazwan Teng, Siaw Lin Tajarudin, Husnul Azan Makhtar, Muaz Mohd Zaini Daud, Zawawi TP155-156 Chemical engineering Microbiologically induced calcium carbonate precipitation (MICCP) has emerged as rather promising green technology compares to conventional calcium carbonate process. Bacillus sphaericus LMG22557 is a superior MICCP-capable microorganism that can potentially utilise urea from diverse sources. This study focuses on maximising and characterising the calcium carbonate produced from fermentation of synthetic urea and waste urea from chicken manure. Morphologies of calcium carbonate attributed from fermentation of urea and precipitation by different calcium source were studied by microscopic observation, Fourier-Transform Infrared (FTIR) and X-Ray Diffraction (XRD). Precipitation from calcium chloride produced more vaterite while precipitation from calcium nitrate tetrahydrate produced pure calcite. Higher specific yield productivity (0.2502 mol/g cell/h) was achieved with 200 rpm and 40 g/L urea concentration. The growth of B. sphaericus in chicken manure solution was achievable but with very low yield of bicarbonate ions (less than 0.0008 mol/g cells/h). In brief, B. sphaericus is able to produce calcium carbonate from synthetic urea while further study is required for chicken manure to be used as substitute. UTHM Publisher 2018 Article PeerReviewed text en http://eprints.uthm.edu.my/5989/1/AJ%202017%20%28937%29.pdf Azmi, Muhammad Syazwan and Teng, Siaw Lin and Tajarudin, Husnul Azan and Makhtar, Muaz Mohd Zaini and Daud, Zawawi (2018) Characterisation of calcium carbonate formed by Bacillus Sphaericus via fermentation of urea. International Journal of Integrated Engineering, 10 (9). pp. 32-37. ISSN 2229-838X
spellingShingle TP155-156 Chemical engineering
Azmi, Muhammad Syazwan
Teng, Siaw Lin
Tajarudin, Husnul Azan
Makhtar, Muaz Mohd Zaini
Daud, Zawawi
Characterisation of calcium carbonate formed by Bacillus Sphaericus via fermentation of urea
title Characterisation of calcium carbonate formed by Bacillus Sphaericus via fermentation of urea
title_full Characterisation of calcium carbonate formed by Bacillus Sphaericus via fermentation of urea
title_fullStr Characterisation of calcium carbonate formed by Bacillus Sphaericus via fermentation of urea
title_full_unstemmed Characterisation of calcium carbonate formed by Bacillus Sphaericus via fermentation of urea
title_short Characterisation of calcium carbonate formed by Bacillus Sphaericus via fermentation of urea
title_sort characterisation of calcium carbonate formed by bacillus sphaericus via fermentation of urea
topic TP155-156 Chemical engineering
url http://eprints.uthm.edu.my/5989/1/AJ%202017%20%28937%29.pdf
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