Identification of bacterial species from longevity spinach leaves

Gynura procumbens or longevity spinach plant is a type of medicinal plant that distributes widely in Southeast Asia including Malaysia, Indonesia, Thailand and the Phillipines. In Malaysia, G. procumbens is commonly called as pokok Sambung Nyawa, Sabong Nyawa, Akar Sebiak or Kecam Akar; and often co...

Full description

Bibliographic Details
Main Authors: Badrulhisham, Nur Sabrina, Solehin, Siti Najihah, Mohamed Rehan, Aisyah, Kamarudin, Kamarudin
Format: Conference or Workshop Item
Language:English
Subjects:
Online Access:http://eprints.uthm.edu.my/6075/1/KP%202020%20%28108%29.pdf
_version_ 1796869302657220608
author Badrulhisham, Nur Sabrina
Solehin, Siti Najihah
Mohamed Rehan, Aisyah
Kamarudin, Kamarudin
author_facet Badrulhisham, Nur Sabrina
Solehin, Siti Najihah
Mohamed Rehan, Aisyah
Kamarudin, Kamarudin
author_sort Badrulhisham, Nur Sabrina
collection UTHM
description Gynura procumbens or longevity spinach plant is a type of medicinal plant that distributes widely in Southeast Asia including Malaysia, Indonesia, Thailand and the Phillipines. In Malaysia, G. procumbens is commonly called as pokok Sambung Nyawa, Sabong Nyawa, Akar Sebiak or Kecam Akar; and often consumed as ulam or salad. Ulam means the food is served raw or freshly uncooked; thus there might be a number of microorganism particularly bacteria that are still inhabiting the leaves. As modern technology is growing rapidly, there is still little to no information in studies and researches focusing on the link between medicinal plants and bacteria that inhabit the plant specifically epiphytic bacteria on the leaf surface. Besides, studies on diversity of bacteria inhabiting the phyllosphere is still unclear. Thus, this study aimed to identify bacteria from the surface of G. procumbens leaves based on morphology through Gram-staining and genetics using 16S ribosomal RNA gene sequencing; and to determine the possible contribution of the bacteria to the medicinal properties of G. procumbens. The findings from this research suggested the presence of five species of bacteria i.e. Bacillus subtilis, Bacillus amyloliquefaciens, Pantoea agglomerans, Sphingomonas melonis and Burkholderia cepacia. From further readings, it is known that some of the species have its potential towards medicinal application such as production of antibiotics. In addition, it is hypothesised that the production of some secondary metabolites in the plant might be due to the presence of the bacteria.
first_indexed 2024-03-05T21:52:51Z
format Conference or Workshop Item
id uthm.eprints-6075
institution Universiti Tun Hussein Onn Malaysia
language English
last_indexed 2024-03-05T21:52:51Z
record_format dspace
spelling uthm.eprints-60752022-01-31T03:29:26Z http://eprints.uthm.edu.my/6075/ Identification of bacterial species from longevity spinach leaves Badrulhisham, Nur Sabrina Solehin, Siti Najihah Mohamed Rehan, Aisyah Kamarudin, Kamarudin TD194-195 Environmental effects of industries and plants TP248.13-248.65 Biotechnology Gynura procumbens or longevity spinach plant is a type of medicinal plant that distributes widely in Southeast Asia including Malaysia, Indonesia, Thailand and the Phillipines. In Malaysia, G. procumbens is commonly called as pokok Sambung Nyawa, Sabong Nyawa, Akar Sebiak or Kecam Akar; and often consumed as ulam or salad. Ulam means the food is served raw or freshly uncooked; thus there might be a number of microorganism particularly bacteria that are still inhabiting the leaves. As modern technology is growing rapidly, there is still little to no information in studies and researches focusing on the link between medicinal plants and bacteria that inhabit the plant specifically epiphytic bacteria on the leaf surface. Besides, studies on diversity of bacteria inhabiting the phyllosphere is still unclear. Thus, this study aimed to identify bacteria from the surface of G. procumbens leaves based on morphology through Gram-staining and genetics using 16S ribosomal RNA gene sequencing; and to determine the possible contribution of the bacteria to the medicinal properties of G. procumbens. The findings from this research suggested the presence of five species of bacteria i.e. Bacillus subtilis, Bacillus amyloliquefaciens, Pantoea agglomerans, Sphingomonas melonis and Burkholderia cepacia. From further readings, it is known that some of the species have its potential towards medicinal application such as production of antibiotics. In addition, it is hypothesised that the production of some secondary metabolites in the plant might be due to the presence of the bacteria. Conference or Workshop Item PeerReviewed text en http://eprints.uthm.edu.my/6075/1/KP%202020%20%28108%29.pdf Badrulhisham, Nur Sabrina and Solehin, Siti Najihah and Mohamed Rehan, Aisyah and Kamarudin, Kamarudin Identification of bacterial species from longevity spinach leaves. In: International Symposium of Straits of Melaka, 30 march 2020, UITM Melaka, Malaysia. (In Press)
spellingShingle TD194-195 Environmental effects of industries and plants
TP248.13-248.65 Biotechnology
Badrulhisham, Nur Sabrina
Solehin, Siti Najihah
Mohamed Rehan, Aisyah
Kamarudin, Kamarudin
Identification of bacterial species from longevity spinach leaves
title Identification of bacterial species from longevity spinach leaves
title_full Identification of bacterial species from longevity spinach leaves
title_fullStr Identification of bacterial species from longevity spinach leaves
title_full_unstemmed Identification of bacterial species from longevity spinach leaves
title_short Identification of bacterial species from longevity spinach leaves
title_sort identification of bacterial species from longevity spinach leaves
topic TD194-195 Environmental effects of industries and plants
TP248.13-248.65 Biotechnology
url http://eprints.uthm.edu.my/6075/1/KP%202020%20%28108%29.pdf
work_keys_str_mv AT badrulhishamnursabrina identificationofbacterialspeciesfromlongevityspinachleaves
AT solehinsitinajihah identificationofbacterialspeciesfromlongevityspinachleaves
AT mohamedrehanaisyah identificationofbacterialspeciesfromlongevityspinachleaves
AT kamarudinkamarudin identificationofbacterialspeciesfromlongevityspinachleaves