The enhancement of plant disease resistance using CRISPR/Cas9 technology

The technologies relating to genome editing have so far progressed rapidly as it has shown the importance of the implementation towards the build up of plan resistance towards pathogens infection. For the past recent years, multiple methods for site directed modification such as clustere...

Full description

Bibliographic Details
Main Authors: Mohd Azman, Amier Asyraf, Mohammad Fouad Iskandar,, Haziq Iskandar, Muhammad Zaidi Leong, Muhamad Nur Hafiz Leong, Mohd Fauzi, Noor Akhmazillah
Format: Book Section
Language:English
Published: Penerbit UTHM 2022
Subjects:
Online Access:http://eprints.uthm.edu.my/7550/1/1.pdf
Description
Summary:The technologies relating to genome editing have so far progressed rapidly as it has shown the importance of the implementation towards the build up of plan resistance towards pathogens infection. For the past recent years, multiple methods for site directed modification such as clustered regularly interspaced short palindrome repeats (CRISPR)/CRISPR�associated protein 9(cas9), zinc finger nucleases (ZFNs) and transcription activator�like effector nucleases (TALENs). Among the example methods, CRISPR/Cas9 has by far produced the best result due to the criteria it possessed such as higher success rate, easier design and implementation, less expensive and more versatile. This chapter will revolve around the CRISPR/Cas9 as how the methods will bring forth the advancement of plant protection against potential diseases. An achievement towards the viral diseases resistant on two main species (Arabidopsis and Nicotiana benthamiana) was highlighted to bring upon discussion. with the viral genome. The modification of crop species such as tomato, wheat, rice and citrus are some of the possible targeted CRISPR/Cas9 modifications.