Total phenolic content and primary antioxidant activity of methanolic and ethanolic extracts of aromatic plants’ leaves

The aim of this study is to determine the total phenolic content and primary antioxidant activity of methanolic and ethanolic extracts of four aromatic plants’ leaves namely knotweed (Polygonum minus), curry (Murraya koenigii), kaffir lime (Citrus hysrix) and fragrant screwpine (Pandanus oduru...

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Main Authors: A. A., Azlim Almey, C., Ahmed Jalal Khan, I., Syed Zahir, K., Mustapha Suleiman, M. R., Aisyah, K., Kamarul Rahim
Format: Article
Language:English
Published: Universiti Putra Malaysia 2010
Subjects:
Online Access:http://eprints.uthm.edu.my/7872/1/J6748_89f03bc09ea4a1c72ebb457f6a2f9e0d.pdf
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author A. A., Azlim Almey
C., Ahmed Jalal Khan
I., Syed Zahir
K., Mustapha Suleiman
M. R., Aisyah
K., Kamarul Rahim
author_facet A. A., Azlim Almey
C., Ahmed Jalal Khan
I., Syed Zahir
K., Mustapha Suleiman
M. R., Aisyah
K., Kamarul Rahim
author_sort A. A., Azlim Almey
collection UTHM
description The aim of this study is to determine the total phenolic content and primary antioxidant activity of methanolic and ethanolic extracts of four aromatic plants’ leaves namely knotweed (Polygonum minus), curry (Murraya koenigii), kaffir lime (Citrus hysrix) and fragrant screwpine (Pandanus odurus). Total phenolic content (TPC) assay using Folin-Ciocalteu method was used to assess the presence and level of phenolic compounds in each sample. The present study showed that both methanolic and ethanolic extracts of P. minus had the highest TPC and followed by M. koenigii, C. hystrix and P. odorus. Primary antioxidant activity in terms of free radical scavenging activities of both methanolic and ethanolic extracts was then measured by 2, 2, diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity assay. The lowest EC50 values based on the DPPH. radical scavenging activity were shown by P. minus extracts as compared to the other samples. For both ethanolic and methanolic extracts, the correlations between TPC and EC50 based on the DPPH. radical scavenging activity assay were negative and weak. Relatively, the present results suggest that of the four aromatic plants, P. minus and M. koenigii have shown potential as sources of natural antioxidants.
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spelling uthm.eprints-78722022-10-17T06:19:19Z http://eprints.uthm.edu.my/7872/ Total phenolic content and primary antioxidant activity of methanolic and ethanolic extracts of aromatic plants’ leaves A. A., Azlim Almey C., Ahmed Jalal Khan I., Syed Zahir K., Mustapha Suleiman M. R., Aisyah K., Kamarul Rahim T Technology (General) The aim of this study is to determine the total phenolic content and primary antioxidant activity of methanolic and ethanolic extracts of four aromatic plants’ leaves namely knotweed (Polygonum minus), curry (Murraya koenigii), kaffir lime (Citrus hysrix) and fragrant screwpine (Pandanus odurus). Total phenolic content (TPC) assay using Folin-Ciocalteu method was used to assess the presence and level of phenolic compounds in each sample. The present study showed that both methanolic and ethanolic extracts of P. minus had the highest TPC and followed by M. koenigii, C. hystrix and P. odorus. Primary antioxidant activity in terms of free radical scavenging activities of both methanolic and ethanolic extracts was then measured by 2, 2, diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity assay. The lowest EC50 values based on the DPPH. radical scavenging activity were shown by P. minus extracts as compared to the other samples. For both ethanolic and methanolic extracts, the correlations between TPC and EC50 based on the DPPH. radical scavenging activity assay were negative and weak. Relatively, the present results suggest that of the four aromatic plants, P. minus and M. koenigii have shown potential as sources of natural antioxidants. Universiti Putra Malaysia 2010 Article PeerReviewed text en http://eprints.uthm.edu.my/7872/1/J6748_89f03bc09ea4a1c72ebb457f6a2f9e0d.pdf A. A., Azlim Almey and C., Ahmed Jalal Khan and I., Syed Zahir and K., Mustapha Suleiman and M. R., Aisyah and K., Kamarul Rahim (2010) Total phenolic content and primary antioxidant activity of methanolic and ethanolic extracts of aromatic plants’ leaves. International Food Research Journal, 17. pp. 1077-1084. ISSN 2231-7546
spellingShingle T Technology (General)
A. A., Azlim Almey
C., Ahmed Jalal Khan
I., Syed Zahir
K., Mustapha Suleiman
M. R., Aisyah
K., Kamarul Rahim
Total phenolic content and primary antioxidant activity of methanolic and ethanolic extracts of aromatic plants’ leaves
title Total phenolic content and primary antioxidant activity of methanolic and ethanolic extracts of aromatic plants’ leaves
title_full Total phenolic content and primary antioxidant activity of methanolic and ethanolic extracts of aromatic plants’ leaves
title_fullStr Total phenolic content and primary antioxidant activity of methanolic and ethanolic extracts of aromatic plants’ leaves
title_full_unstemmed Total phenolic content and primary antioxidant activity of methanolic and ethanolic extracts of aromatic plants’ leaves
title_short Total phenolic content and primary antioxidant activity of methanolic and ethanolic extracts of aromatic plants’ leaves
title_sort total phenolic content and primary antioxidant activity of methanolic and ethanolic extracts of aromatic plants leaves
topic T Technology (General)
url http://eprints.uthm.edu.my/7872/1/J6748_89f03bc09ea4a1c72ebb457f6a2f9e0d.pdf
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