Development of high voltage pulse inducement method for biological cell
— Electroporation (EP) system is a process of controlling cell functions by using electromagnetic fields (EMF) to create pores through a cellular membrane that causes cell lysis and apoptosis. In this paper we present an experimental setup for fundamental studies on cell EP. An adjustable hi...
| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2012
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| Subjects: | |
| Online Access: | http://eprints.uthm.edu.my/8064/1/P8721_d7eb1fc9936f85924360524c7e73231f.pdf |
| _version_ | 1796869693038919680 |
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| author | Adon, Mohamad Nazib Dalimin, M. Noh Abdul Jamil, Muhammad Mahadi Mohd Kassim, Norazan |
| author_facet | Adon, Mohamad Nazib Dalimin, M. Noh Abdul Jamil, Muhammad Mahadi Mohd Kassim, Norazan |
| author_sort | Adon, Mohamad Nazib |
| collection | UTHM |
| description | — Electroporation (EP) system is a process of controlling
cell functions by using electromagnetic fields (EMF) to create
pores through a cellular membrane that causes cell lysis and
apoptosis. In this paper we present an experimental setup for
fundamental studies on cell EP. An adjustable high voltage pulse
generator (3kV/10μs – 600μs pulse length) system were connected to
the EP chamber which subsequently allow real time observation
of membrane permeability changes and cellular physiology.
In order to initiate higher cell viability rate, high transfection
efficiency, lower sample contamination and smaller Joule heating
the modification of EP chamber need to be implemented. .
Following that, HeLa cell culture has been projected as cell that
will be used in this study. Finally, some suggestions are proposed
for the future studies. |
| first_indexed | 2024-03-05T21:58:34Z |
| format | Conference or Workshop Item |
| id | uthm.eprints-8064 |
| institution | Universiti Tun Hussein Onn Malaysia |
| language | English |
| last_indexed | 2024-03-05T21:58:34Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | uthm.eprints-80642022-12-06T02:54:58Z http://eprints.uthm.edu.my/8064/ Development of high voltage pulse inducement method for biological cell Adon, Mohamad Nazib Dalimin, M. Noh Abdul Jamil, Muhammad Mahadi Mohd Kassim, Norazan T Technology (General) — Electroporation (EP) system is a process of controlling cell functions by using electromagnetic fields (EMF) to create pores through a cellular membrane that causes cell lysis and apoptosis. In this paper we present an experimental setup for fundamental studies on cell EP. An adjustable high voltage pulse generator (3kV/10μs – 600μs pulse length) system were connected to the EP chamber which subsequently allow real time observation of membrane permeability changes and cellular physiology. In order to initiate higher cell viability rate, high transfection efficiency, lower sample contamination and smaller Joule heating the modification of EP chamber need to be implemented. . Following that, HeLa cell culture has been projected as cell that will be used in this study. Finally, some suggestions are proposed for the future studies. 2012 Conference or Workshop Item PeerReviewed text en http://eprints.uthm.edu.my/8064/1/P8721_d7eb1fc9936f85924360524c7e73231f.pdf Adon, Mohamad Nazib and Dalimin, M. Noh and Abdul Jamil, Muhammad Mahadi and Mohd Kassim, Norazan (2012) Development of high voltage pulse inducement method for biological cell. In: 2012 International Conference on Biomedical Engineering (ICoBE), 27-28 February 2012, Penang. |
| spellingShingle | T Technology (General) Adon, Mohamad Nazib Dalimin, M. Noh Abdul Jamil, Muhammad Mahadi Mohd Kassim, Norazan Development of high voltage pulse inducement method for biological cell |
| title | Development of high voltage pulse inducement method for biological cell |
| title_full | Development of high voltage pulse inducement method for biological cell |
| title_fullStr | Development of high voltage pulse inducement method for biological cell |
| title_full_unstemmed | Development of high voltage pulse inducement method for biological cell |
| title_short | Development of high voltage pulse inducement method for biological cell |
| title_sort | development of high voltage pulse inducement method for biological cell |
| topic | T Technology (General) |
| url | http://eprints.uthm.edu.my/8064/1/P8721_d7eb1fc9936f85924360524c7e73231f.pdf |
| work_keys_str_mv | AT adonmohamadnazib developmentofhighvoltagepulseinducementmethodforbiologicalcell AT daliminmnoh developmentofhighvoltagepulseinducementmethodforbiologicalcell AT abduljamilmuhammadmahadi developmentofhighvoltagepulseinducementmethodforbiologicalcell AT mohdkassimnorazan developmentofhighvoltagepulseinducementmethodforbiologicalcell |