Antioxidant and anticholinesterase activities of centella asiatica phenolic extract-mediated gold nanoparticles
In the present study, the green synthesis and optimization of gold nanoparticles (GNPs) using C. asiatica phenolic extract (CAP) were reported followed by the characterization with UV-vis, FTIR, Zeta potential, FESEM, EDX and XRD analyses. Total phenolic and flavonoid contents (TPC and TFC), antioxi...
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| Format: | Thesis |
| Language: | English English |
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2019
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| Online Access: | http://eprints.uthm.edu.my/91/1/24p%20MUHAMMAD%20SOHAIL%20LATIF.pdf http://eprints.uthm.edu.my/91/2/MUHAMMAD%20SOHAIL%20LATIF%20WATERMARK.pdf |
| _version_ | 1796868204691193856 |
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| author | Latif, Muhammad Sohail |
| author_facet | Latif, Muhammad Sohail |
| author_sort | Latif, Muhammad Sohail |
| collection | UTHM |
| description | In the present study, the green synthesis and optimization of gold nanoparticles (GNPs) using C. asiatica phenolic extract (CAP) were reported followed by the characterization with UV-vis, FTIR, Zeta potential, FESEM, EDX and XRD analyses. Total phenolic and flavonoid contents (TPC and TFC), antioxidant and anticholinesterase (anti-ChE) activities were also tested in vitro and Pearson correlation analysis was performed. The GNP synthesis using CAP was successfully accomplished under optimum conditions of reactants ratio of 1:1, 8 % CAP concentration, 0.5 mM HAuCl4 concentration and pH 9 at different temperatures of reaction medium (25 ℃, 40 ℃, 55 ℃ and 70 ℃). The results showed that the size range of GNPs was reduced from 6-40 nm at 25 ℃ to 6-30 nm at 40 ℃ and 55 ℃ while the sphericity of GNPs increased and the surface plasmon resonance (SPR) band became narrow. Although all GNP samples showed good antioxidant and anti-ChE activities (% I), the highest activity was recorded for the GNPs synthesized at 55 ℃, that is, 69.06 % and 76.72 % for ABTS and DPPH, and 71.71 % and 69.88 % for AChE and BChE, respectively, at the highest concentration of 500 μL sample. However, the maximum % I for ABTS and DPPH scavenging (79.76±1.01 and 75.51±1.54, respectively) was shown by ascorbic acid, and for AChE and BChE inhibition (75.18±2.29 and 79.94±1.11, respectively) by galanthamine. Pearson correlation analyses revealed a strong positive correlation between antioxidant activity and phenolic content as well as between anti-ChE and antioxidant activities for all GNP samples. The GNPs did not possess a significant cytotoxicity as shown by 20% mortality in brine shrimp lethality assay. The GNPs were haemocompatible as they induced 0.92 % haemolysis at the highest concentration (500 μL) in haemolysis assay compared to 99 % haemolysis by positive control. In conclusion, the results of this study show that CAP-GNPs possess good antioxidant and anticholinesterase activities, in addition to being non-cytotoxic and non-haemolytic, may be an option to further explore their therapeutic potential as antioxidant and cholinesterase inhibitors. |
| first_indexed | 2024-03-05T21:36:20Z |
| format | Thesis |
| id | uthm.eprints-91 |
| institution | Universiti Tun Hussein Onn Malaysia |
| language | English English |
| last_indexed | 2024-03-05T21:36:20Z |
| publishDate | 2019 |
| record_format | dspace |
| spelling | uthm.eprints-912021-06-22T07:52:26Z http://eprints.uthm.edu.my/91/ Antioxidant and anticholinesterase activities of centella asiatica phenolic extract-mediated gold nanoparticles Latif, Muhammad Sohail TX341-641 Nutrition. Foods and food supply In the present study, the green synthesis and optimization of gold nanoparticles (GNPs) using C. asiatica phenolic extract (CAP) were reported followed by the characterization with UV-vis, FTIR, Zeta potential, FESEM, EDX and XRD analyses. Total phenolic and flavonoid contents (TPC and TFC), antioxidant and anticholinesterase (anti-ChE) activities were also tested in vitro and Pearson correlation analysis was performed. The GNP synthesis using CAP was successfully accomplished under optimum conditions of reactants ratio of 1:1, 8 % CAP concentration, 0.5 mM HAuCl4 concentration and pH 9 at different temperatures of reaction medium (25 ℃, 40 ℃, 55 ℃ and 70 ℃). The results showed that the size range of GNPs was reduced from 6-40 nm at 25 ℃ to 6-30 nm at 40 ℃ and 55 ℃ while the sphericity of GNPs increased and the surface plasmon resonance (SPR) band became narrow. Although all GNP samples showed good antioxidant and anti-ChE activities (% I), the highest activity was recorded for the GNPs synthesized at 55 ℃, that is, 69.06 % and 76.72 % for ABTS and DPPH, and 71.71 % and 69.88 % for AChE and BChE, respectively, at the highest concentration of 500 μL sample. However, the maximum % I for ABTS and DPPH scavenging (79.76±1.01 and 75.51±1.54, respectively) was shown by ascorbic acid, and for AChE and BChE inhibition (75.18±2.29 and 79.94±1.11, respectively) by galanthamine. Pearson correlation analyses revealed a strong positive correlation between antioxidant activity and phenolic content as well as between anti-ChE and antioxidant activities for all GNP samples. The GNPs did not possess a significant cytotoxicity as shown by 20% mortality in brine shrimp lethality assay. The GNPs were haemocompatible as they induced 0.92 % haemolysis at the highest concentration (500 μL) in haemolysis assay compared to 99 % haemolysis by positive control. In conclusion, the results of this study show that CAP-GNPs possess good antioxidant and anticholinesterase activities, in addition to being non-cytotoxic and non-haemolytic, may be an option to further explore their therapeutic potential as antioxidant and cholinesterase inhibitors. 2019-07 Thesis NonPeerReviewed text en http://eprints.uthm.edu.my/91/1/24p%20MUHAMMAD%20SOHAIL%20LATIF.pdf text en http://eprints.uthm.edu.my/91/2/MUHAMMAD%20SOHAIL%20LATIF%20WATERMARK.pdf Latif, Muhammad Sohail (2019) Antioxidant and anticholinesterase activities of centella asiatica phenolic extract-mediated gold nanoparticles. Doctoral thesis, Universiti Tun Hussein Onn Malaysia. |
| spellingShingle | TX341-641 Nutrition. Foods and food supply Latif, Muhammad Sohail Antioxidant and anticholinesterase activities of centella asiatica phenolic extract-mediated gold nanoparticles |
| title | Antioxidant and anticholinesterase activities of centella asiatica phenolic extract-mediated gold nanoparticles |
| title_full | Antioxidant and anticholinesterase activities of centella asiatica phenolic extract-mediated gold nanoparticles |
| title_fullStr | Antioxidant and anticholinesterase activities of centella asiatica phenolic extract-mediated gold nanoparticles |
| title_full_unstemmed | Antioxidant and anticholinesterase activities of centella asiatica phenolic extract-mediated gold nanoparticles |
| title_short | Antioxidant and anticholinesterase activities of centella asiatica phenolic extract-mediated gold nanoparticles |
| title_sort | antioxidant and anticholinesterase activities of centella asiatica phenolic extract mediated gold nanoparticles |
| topic | TX341-641 Nutrition. Foods and food supply |
| url | http://eprints.uthm.edu.my/91/1/24p%20MUHAMMAD%20SOHAIL%20LATIF.pdf http://eprints.uthm.edu.my/91/2/MUHAMMAD%20SOHAIL%20LATIF%20WATERMARK.pdf |
| work_keys_str_mv | AT latifmuhammadsohail antioxidantandanticholinesteraseactivitiesofcentellaasiaticaphenolicextractmediatedgoldnanoparticles |