Cross-linked cyclodextrin glucanotransferase aggregates from Bacillus lehensis G1 for cyclodextrin production: Molecular modeling, developmental, physicochemical, kinetic and thermodynamic properties
Type of cross-linking agents influence the stability and active cross-linked enzyme aggregates (CLEA) immobilization. The information of molecular interaction between enzyme-cross linker is not well explored thus screening wide numbers of cross-linker is crucial in CLEA development. This study combi...
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| Format: | Article |
| Language: | English |
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Elsevier B.V.
2022
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| Online Access: | http://eprints.utm.my/100998/1/NashriqJailani2022_CrosslinkedCyclodextrinGlucanotransferaseAggregate.pdf |
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| author | Jailani, Nashriq Jaafar, Nardiah Rizwana Suhaimi, Suhaily Mackeen, Mukram Mohamed Abu Bakar, Farah Diba |
| author_facet | Jailani, Nashriq Jaafar, Nardiah Rizwana Suhaimi, Suhaily Mackeen, Mukram Mohamed Abu Bakar, Farah Diba |
| author_sort | Jailani, Nashriq |
| collection | ePrints |
| description | Type of cross-linking agents influence the stability and active cross-linked enzyme aggregates (CLEA) immobilization. The information of molecular interaction between enzyme-cross linker is not well explored thus screening wide numbers of cross-linker is crucial in CLEA development. This study combined the molecular modeling and experimental optimization to investigate the influences of different cross-linking agents in developing CLEA of cyclodextrin glucanotranferase G1 (CGTase G1) for cyclodextrins (CDs) synthesis. Seven types of cross-linkers were tested and CGTase G1 cross-linked with chitosan (CS-CGTG1-CLEA) displayed the highest activity recovery (84.6 ± 0.26%), aligning with its highest binding affinity, radius of gyration and flexibility through in-silico analysis towards CGTase G1. CS-CGTG1-CLEA was characterized and showed a longer half-life (30.06 ± 1.51 min) and retained a greater thermal stability (52.73 ± 0.93%) after 30 min incubation at optimal conditions compared to free enzyme (10.30 ± 1.34 min and 5.51 ± 2.10% respectively). CS-CGTG1-CLEA improved CDs production by 33% and yielded cumulative of 52.62 g/L CDs after five cycles for 2 h of reaction. This study reveals that abundant of hydroxyl group on chitosan interacted with CGTase G1 surface amino acid residues to form strong and stable CLEA thus can be a promising biocatalyst in CDs production. |
| first_indexed | 2024-03-05T21:20:19Z |
| format | Article |
| id | utm.eprints-100998 |
| institution | Universiti Teknologi Malaysia - ePrints |
| language | English |
| last_indexed | 2024-03-05T21:20:19Z |
| publishDate | 2022 |
| publisher | Elsevier B.V. |
| record_format | dspace |
| spelling | utm.eprints-1009982023-05-18T06:13:38Z http://eprints.utm.my/100998/ Cross-linked cyclodextrin glucanotransferase aggregates from Bacillus lehensis G1 for cyclodextrin production: Molecular modeling, developmental, physicochemical, kinetic and thermodynamic properties Jailani, Nashriq Jaafar, Nardiah Rizwana Suhaimi, Suhaily Mackeen, Mukram Mohamed Abu Bakar, Farah Diba TP Chemical technology Type of cross-linking agents influence the stability and active cross-linked enzyme aggregates (CLEA) immobilization. The information of molecular interaction between enzyme-cross linker is not well explored thus screening wide numbers of cross-linker is crucial in CLEA development. This study combined the molecular modeling and experimental optimization to investigate the influences of different cross-linking agents in developing CLEA of cyclodextrin glucanotranferase G1 (CGTase G1) for cyclodextrins (CDs) synthesis. Seven types of cross-linkers were tested and CGTase G1 cross-linked with chitosan (CS-CGTG1-CLEA) displayed the highest activity recovery (84.6 ± 0.26%), aligning with its highest binding affinity, radius of gyration and flexibility through in-silico analysis towards CGTase G1. CS-CGTG1-CLEA was characterized and showed a longer half-life (30.06 ± 1.51 min) and retained a greater thermal stability (52.73 ± 0.93%) after 30 min incubation at optimal conditions compared to free enzyme (10.30 ± 1.34 min and 5.51 ± 2.10% respectively). CS-CGTG1-CLEA improved CDs production by 33% and yielded cumulative of 52.62 g/L CDs after five cycles for 2 h of reaction. This study reveals that abundant of hydroxyl group on chitosan interacted with CGTase G1 surface amino acid residues to form strong and stable CLEA thus can be a promising biocatalyst in CDs production. Elsevier B.V. 2022 Article PeerReviewed application/pdf en http://eprints.utm.my/100998/1/NashriqJailani2022_CrosslinkedCyclodextrinGlucanotransferaseAggregate.pdf Jailani, Nashriq and Jaafar, Nardiah Rizwana and Suhaimi, Suhaily and Mackeen, Mukram Mohamed and Abu Bakar, Farah Diba (2022) Cross-linked cyclodextrin glucanotransferase aggregates from Bacillus lehensis G1 for cyclodextrin production: Molecular modeling, developmental, physicochemical, kinetic and thermodynamic properties. International Journal of Biological Macromolecules, 213 (-). pp. 516-533. ISSN 0141-8130 http://dx.doi.org/10.1016/j.ijbiomac.2022.05.170 DOI: 10.1016/j.ijbiomac.2022.05.170 |
| spellingShingle | TP Chemical technology Jailani, Nashriq Jaafar, Nardiah Rizwana Suhaimi, Suhaily Mackeen, Mukram Mohamed Abu Bakar, Farah Diba Cross-linked cyclodextrin glucanotransferase aggregates from Bacillus lehensis G1 for cyclodextrin production: Molecular modeling, developmental, physicochemical, kinetic and thermodynamic properties |
| title | Cross-linked cyclodextrin glucanotransferase aggregates from Bacillus lehensis G1 for cyclodextrin production: Molecular modeling, developmental, physicochemical, kinetic and thermodynamic properties |
| title_full | Cross-linked cyclodextrin glucanotransferase aggregates from Bacillus lehensis G1 for cyclodextrin production: Molecular modeling, developmental, physicochemical, kinetic and thermodynamic properties |
| title_fullStr | Cross-linked cyclodextrin glucanotransferase aggregates from Bacillus lehensis G1 for cyclodextrin production: Molecular modeling, developmental, physicochemical, kinetic and thermodynamic properties |
| title_full_unstemmed | Cross-linked cyclodextrin glucanotransferase aggregates from Bacillus lehensis G1 for cyclodextrin production: Molecular modeling, developmental, physicochemical, kinetic and thermodynamic properties |
| title_short | Cross-linked cyclodextrin glucanotransferase aggregates from Bacillus lehensis G1 for cyclodextrin production: Molecular modeling, developmental, physicochemical, kinetic and thermodynamic properties |
| title_sort | cross linked cyclodextrin glucanotransferase aggregates from bacillus lehensis g1 for cyclodextrin production molecular modeling developmental physicochemical kinetic and thermodynamic properties |
| topic | TP Chemical technology |
| url | http://eprints.utm.my/100998/1/NashriqJailani2022_CrosslinkedCyclodextrinGlucanotransferaseAggregate.pdf |
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