Efficacious bioconversion of waste walnut shells to xylotetrose and xylopentose by free xylanase (Xy) and MOF immobilized xylanase (Xy-Cu-BTC)
This study uses a cost effective and efficient method for production of higher DP (degree of polymerization) Xylooligosaccharides (XOS) from xylan extracted from the waste walnut shells. Copper based metal organic framework (Cu-BTC MOF) was prepared for immobilization of free xylanase (Xy) enzyme by...
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| Format: | Article |
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Elsevier Ltd
2022
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| _version_ | 1796867029146271744 |
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| author | Kaushal, Jyoti Arya, Shailendra Kumar Khatri, Madhu Singh, Gursharan Wan Azelee, Nur Izyan Rajagopal, Rajinikanth Chang, Soon Woong Ravindran, Balasubramani Awasthi, Mukesh Kumar |
| author_facet | Kaushal, Jyoti Arya, Shailendra Kumar Khatri, Madhu Singh, Gursharan Wan Azelee, Nur Izyan Rajagopal, Rajinikanth Chang, Soon Woong Ravindran, Balasubramani Awasthi, Mukesh Kumar |
| author_sort | Kaushal, Jyoti |
| collection | ePrints |
| description | This study uses a cost effective and efficient method for production of higher DP (degree of polymerization) Xylooligosaccharides (XOS) from xylan extracted from the waste walnut shells. Copper based metal organic framework (Cu-BTC MOF) was prepared for immobilization of free xylanase (Xy) enzyme by green synthesis method. Both free and immobilized xylanase (Xy-Cu-BTC) were able to cause the bioconversion of xylan (87.4% yield) into XOS. Predominant production of xylotetrose (X4) and xylopentose (X5) was observed for both the methods. Percentage XOS conversion for free enzyme (Xy) was found to be 4.1% X4 and 60.57% X5 whereas these values increased in case of immobilized system where 11.8% X4 and 64.2% X5 were produced. Xylose production was minute in case of immobilized xylanase 0.88% which makes it a better method for XOS production free from xylose interference. Xy-Cu-BTC MOF can hence be used as an attractive alternative for pure XOS production. |
| first_indexed | 2024-03-05T21:21:03Z |
| format | Article |
| id | utm.eprints-101249 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T21:21:03Z |
| publishDate | 2022 |
| publisher | Elsevier Ltd |
| record_format | dspace |
| spelling | utm.eprints-1012492023-11-13T06:18:43Z http://eprints.utm.my/101249/ Efficacious bioconversion of waste walnut shells to xylotetrose and xylopentose by free xylanase (Xy) and MOF immobilized xylanase (Xy-Cu-BTC) Kaushal, Jyoti Arya, Shailendra Kumar Khatri, Madhu Singh, Gursharan Wan Azelee, Nur Izyan Rajagopal, Rajinikanth Chang, Soon Woong Ravindran, Balasubramani Awasthi, Mukesh Kumar TP Chemical technology This study uses a cost effective and efficient method for production of higher DP (degree of polymerization) Xylooligosaccharides (XOS) from xylan extracted from the waste walnut shells. Copper based metal organic framework (Cu-BTC MOF) was prepared for immobilization of free xylanase (Xy) enzyme by green synthesis method. Both free and immobilized xylanase (Xy-Cu-BTC) were able to cause the bioconversion of xylan (87.4% yield) into XOS. Predominant production of xylotetrose (X4) and xylopentose (X5) was observed for both the methods. Percentage XOS conversion for free enzyme (Xy) was found to be 4.1% X4 and 60.57% X5 whereas these values increased in case of immobilized system where 11.8% X4 and 64.2% X5 were produced. Xylose production was minute in case of immobilized xylanase 0.88% which makes it a better method for XOS production free from xylose interference. Xy-Cu-BTC MOF can hence be used as an attractive alternative for pure XOS production. Elsevier Ltd 2022 Article PeerReviewed Kaushal, Jyoti and Arya, Shailendra Kumar and Khatri, Madhu and Singh, Gursharan and Wan Azelee, Nur Izyan and Rajagopal, Rajinikanth and Chang, Soon Woong and Ravindran, Balasubramani and Awasthi, Mukesh Kumar (2022) Efficacious bioconversion of waste walnut shells to xylotetrose and xylopentose by free xylanase (Xy) and MOF immobilized xylanase (Xy-Cu-BTC). Bioresource Technology, 357 (NA). pp. 1-5. ISSN 0960-8524 http://dx.doi.org/10.1016/j.biortech.2022.127374 DOI : 10.1016/j.biortech.2022.127374 |
| spellingShingle | TP Chemical technology Kaushal, Jyoti Arya, Shailendra Kumar Khatri, Madhu Singh, Gursharan Wan Azelee, Nur Izyan Rajagopal, Rajinikanth Chang, Soon Woong Ravindran, Balasubramani Awasthi, Mukesh Kumar Efficacious bioconversion of waste walnut shells to xylotetrose and xylopentose by free xylanase (Xy) and MOF immobilized xylanase (Xy-Cu-BTC) |
| title | Efficacious bioconversion of waste walnut shells to xylotetrose and xylopentose by free xylanase (Xy) and MOF immobilized xylanase (Xy-Cu-BTC) |
| title_full | Efficacious bioconversion of waste walnut shells to xylotetrose and xylopentose by free xylanase (Xy) and MOF immobilized xylanase (Xy-Cu-BTC) |
| title_fullStr | Efficacious bioconversion of waste walnut shells to xylotetrose and xylopentose by free xylanase (Xy) and MOF immobilized xylanase (Xy-Cu-BTC) |
| title_full_unstemmed | Efficacious bioconversion of waste walnut shells to xylotetrose and xylopentose by free xylanase (Xy) and MOF immobilized xylanase (Xy-Cu-BTC) |
| title_short | Efficacious bioconversion of waste walnut shells to xylotetrose and xylopentose by free xylanase (Xy) and MOF immobilized xylanase (Xy-Cu-BTC) |
| title_sort | efficacious bioconversion of waste walnut shells to xylotetrose and xylopentose by free xylanase xy and mof immobilized xylanase xy cu btc |
| topic | TP Chemical technology |
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