Electroactive polymeric nanocomposite BC-g-(Fe3O4/GO) materials for bone tissue engineering: in vitro evaluations
Tissue engineering is a cutting-edge approach for using advanced biomaterials to treat defective bone to get desired clinical results. In bone tissue engineering, the scaffolds must have the desired physicochemical and biomechanical natural properties in order to regenerate complicated defective bon...
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| Format: | Article |
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Taylor and Francis Ltd.
2022
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| author | Aslam Khan, Muhammad Umar Rizwan, Muhammad Abd. Razak, Saiful Izwan Hassan, Anwarul Rasheed, Tahir Muhammad Bilal, Muhammad Bilal |
| author_facet | Aslam Khan, Muhammad Umar Rizwan, Muhammad Abd. Razak, Saiful Izwan Hassan, Anwarul Rasheed, Tahir Muhammad Bilal, Muhammad Bilal |
| author_sort | Aslam Khan, Muhammad Umar |
| collection | ePrints |
| description | Tissue engineering is a cutting-edge approach for using advanced biomaterials to treat defective bone to get desired clinical results. In bone tissue engineering, the scaffolds must have the desired physicochemical and biomechanical natural properties in order to regenerate complicated defective bone. For the first time, polymeric nanocomposite material was developed using cellulose and co-dispersed nanosystem (Fe3O4/GO) by free radical polymerization to fabricate porous polymeric scaffolds via freeze drying. Various characterizations techniques, such as Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM)/energy dispersive X-ray (EDX), and universal testing machine (UTM) were used to investigate structural, morphological, and mechanical properties. Swelling, biodegradation, and wetting analysis were also performed to evaluate their physicochemical behavior. Intercalation of Fe3O4 nanoparticles into GO-sheets promoted their dispersion into the polymeric matrix. All porous scaffolds possessed a well-interconnected porous structure, while the synergistic effect of Fe3O4/GO reinforces the mechanical strength of porous scaffolds. The compressive strength and Young’s modulus were increased by increasing Fe3O4 amount, and maximum mechanical strength was found in HFG-4 and least in HFG-1. However, these porous scaffolds have different swelling and biodegradation behavior due to the variable Fe3O4 intercalations into GO-sheets. Antibacterial activities of porous scaffolds were studied against severe Gram-positive and Gram-negative pathogens and increased Fe3O4 amount in nanosystem increased the antibacterial activities. The cell viability and morphology of pre-osteoblast (MC3T3-E1) cell lines were studied against porous scaffolds and increased cell viability and proliferation were observed from HFG-1 to HFG-4. Hence, the electroactive material could be the potential material for bone tissue engineering. |
| first_indexed | 2024-03-05T21:26:01Z |
| format | Article |
| id | utm.eprints-102908 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T21:26:01Z |
| publishDate | 2022 |
| publisher | Taylor and Francis Ltd. |
| record_format | dspace |
| spelling | utm.eprints-1029082023-09-26T06:22:21Z http://eprints.utm.my/102908/ Electroactive polymeric nanocomposite BC-g-(Fe3O4/GO) materials for bone tissue engineering: in vitro evaluations Aslam Khan, Muhammad Umar Rizwan, Muhammad Abd. Razak, Saiful Izwan Hassan, Anwarul Rasheed, Tahir Muhammad Bilal, Muhammad Bilal Q Science (General) Tissue engineering is a cutting-edge approach for using advanced biomaterials to treat defective bone to get desired clinical results. In bone tissue engineering, the scaffolds must have the desired physicochemical and biomechanical natural properties in order to regenerate complicated defective bone. For the first time, polymeric nanocomposite material was developed using cellulose and co-dispersed nanosystem (Fe3O4/GO) by free radical polymerization to fabricate porous polymeric scaffolds via freeze drying. Various characterizations techniques, such as Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscope (SEM)/energy dispersive X-ray (EDX), and universal testing machine (UTM) were used to investigate structural, morphological, and mechanical properties. Swelling, biodegradation, and wetting analysis were also performed to evaluate their physicochemical behavior. Intercalation of Fe3O4 nanoparticles into GO-sheets promoted their dispersion into the polymeric matrix. All porous scaffolds possessed a well-interconnected porous structure, while the synergistic effect of Fe3O4/GO reinforces the mechanical strength of porous scaffolds. The compressive strength and Young’s modulus were increased by increasing Fe3O4 amount, and maximum mechanical strength was found in HFG-4 and least in HFG-1. However, these porous scaffolds have different swelling and biodegradation behavior due to the variable Fe3O4 intercalations into GO-sheets. Antibacterial activities of porous scaffolds were studied against severe Gram-positive and Gram-negative pathogens and increased Fe3O4 amount in nanosystem increased the antibacterial activities. The cell viability and morphology of pre-osteoblast (MC3T3-E1) cell lines were studied against porous scaffolds and increased cell viability and proliferation were observed from HFG-1 to HFG-4. Hence, the electroactive material could be the potential material for bone tissue engineering. Taylor and Francis Ltd. 2022 Article PeerReviewed Aslam Khan, Muhammad Umar and Rizwan, Muhammad and Abd. Razak, Saiful Izwan and Hassan, Anwarul and Rasheed, Tahir and Muhammad Bilal, Muhammad Bilal (2022) Electroactive polymeric nanocomposite BC-g-(Fe3O4/GO) materials for bone tissue engineering: in vitro evaluations. Journal of Biomaterials Science, Polymer Edition, 33 (11). pp. 1349-1368. ISSN 0920-5063 http://dx.doi.org/10.1080/09205063.2022.2054544 DOI: 10.1080/09205063.2022.2054544 |
| spellingShingle | Q Science (General) Aslam Khan, Muhammad Umar Rizwan, Muhammad Abd. Razak, Saiful Izwan Hassan, Anwarul Rasheed, Tahir Muhammad Bilal, Muhammad Bilal Electroactive polymeric nanocomposite BC-g-(Fe3O4/GO) materials for bone tissue engineering: in vitro evaluations |
| title | Electroactive polymeric nanocomposite BC-g-(Fe3O4/GO) materials for bone tissue engineering: in vitro evaluations |
| title_full | Electroactive polymeric nanocomposite BC-g-(Fe3O4/GO) materials for bone tissue engineering: in vitro evaluations |
| title_fullStr | Electroactive polymeric nanocomposite BC-g-(Fe3O4/GO) materials for bone tissue engineering: in vitro evaluations |
| title_full_unstemmed | Electroactive polymeric nanocomposite BC-g-(Fe3O4/GO) materials for bone tissue engineering: in vitro evaluations |
| title_short | Electroactive polymeric nanocomposite BC-g-(Fe3O4/GO) materials for bone tissue engineering: in vitro evaluations |
| title_sort | electroactive polymeric nanocomposite bc g fe3o4 go materials for bone tissue engineering in vitro evaluations |
| topic | Q Science (General) |
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