Antibacterial activity and cell responses of vancomycin-loaded alginate coating on ZSM-5 Scaffold for bone tissue engineering applications
Despite the significant advancement in bone tissue engineering, it is still challenging to find a desired scaffold with suitable mechanical and biological properties, efficient bone formation in the defect area, and antibacterial resistivity. In this study, the zeolite (ZSM-5) scaffold was developed...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI
2022
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| Online Access: | http://eprints.utm.my/102934/1/AhamdFauziIsmail2022_AntibacterialActivityandCellResponses.pdf |
| _version_ | 1796867361700052992 |
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| author | Aslani, Zahra Nazemi, N. Rajabi, Negar Kharaziha, Mahshid Bakhsheshi Rad, Hamid Reza Kasiri Asgarani, M. Najafinezhad, Aliakbar Ismail, Ahamd Fauzi Shari, S. Berto, Filippo |
| author_facet | Aslani, Zahra Nazemi, N. Rajabi, Negar Kharaziha, Mahshid Bakhsheshi Rad, Hamid Reza Kasiri Asgarani, M. Najafinezhad, Aliakbar Ismail, Ahamd Fauzi Shari, S. Berto, Filippo |
| author_sort | Aslani, Zahra |
| collection | ePrints |
| description | Despite the significant advancement in bone tissue engineering, it is still challenging to find a desired scaffold with suitable mechanical and biological properties, efficient bone formation in the defect area, and antibacterial resistivity. In this study, the zeolite (ZSM-5) scaffold was developed using the space holder method, and a novel vancomycin-loaded alginate coating was developed on it to promote their characteristics. Our results demonstrated the importance of alginate coating on the microstructure, mechanical, and cellular properties of the ZSM-5 scaffold. For instance, a three-fold increase in the compressive strength of coated scaffolds was observed compared to the uncoated ZSM-5. After the incorporation of vancomycin into the alginate coating, the scaffold revealed significant antibacterial activity against Staphylococcus aureus (S. aureus). The inhibition zone increased to 35 mm. Resets also demonstrated 74 ± 2.5% porosity, 4.3 ± 0.07 MPa strength in compressive conditions, acceptable cellular properties (72.3 ± 0.2 (%control) cell viability) after 7 days, good cell attachment, and calcium deposition. Overall, the results revealed that this scaffold could be a great candidate for bone tissue engineering. |
| first_indexed | 2024-03-05T21:26:05Z |
| format | Article |
| id | utm.eprints-102934 |
| institution | Universiti Teknologi Malaysia - ePrints |
| language | English |
| last_indexed | 2024-03-05T21:26:05Z |
| publishDate | 2022 |
| publisher | MDPI |
| record_format | dspace |
| spelling | utm.eprints-1029342023-10-12T08:15:41Z http://eprints.utm.my/102934/ Antibacterial activity and cell responses of vancomycin-loaded alginate coating on ZSM-5 Scaffold for bone tissue engineering applications Aslani, Zahra Nazemi, N. Rajabi, Negar Kharaziha, Mahshid Bakhsheshi Rad, Hamid Reza Kasiri Asgarani, M. Najafinezhad, Aliakbar Ismail, Ahamd Fauzi Shari, S. Berto, Filippo TJ Mechanical engineering and machinery TP Chemical technology Despite the significant advancement in bone tissue engineering, it is still challenging to find a desired scaffold with suitable mechanical and biological properties, efficient bone formation in the defect area, and antibacterial resistivity. In this study, the zeolite (ZSM-5) scaffold was developed using the space holder method, and a novel vancomycin-loaded alginate coating was developed on it to promote their characteristics. Our results demonstrated the importance of alginate coating on the microstructure, mechanical, and cellular properties of the ZSM-5 scaffold. For instance, a three-fold increase in the compressive strength of coated scaffolds was observed compared to the uncoated ZSM-5. After the incorporation of vancomycin into the alginate coating, the scaffold revealed significant antibacterial activity against Staphylococcus aureus (S. aureus). The inhibition zone increased to 35 mm. Resets also demonstrated 74 ± 2.5% porosity, 4.3 ± 0.07 MPa strength in compressive conditions, acceptable cellular properties (72.3 ± 0.2 (%control) cell viability) after 7 days, good cell attachment, and calcium deposition. Overall, the results revealed that this scaffold could be a great candidate for bone tissue engineering. MDPI 2022-07-02 Article PeerReviewed application/pdf en http://eprints.utm.my/102934/1/AhamdFauziIsmail2022_AntibacterialActivityandCellResponses.pdf Aslani, Zahra and Nazemi, N. and Rajabi, Negar and Kharaziha, Mahshid and Bakhsheshi Rad, Hamid Reza and Kasiri Asgarani, M. and Najafinezhad, Aliakbar and Ismail, Ahamd Fauzi and Shari, S. and Berto, Filippo (2022) Antibacterial activity and cell responses of vancomycin-loaded alginate coating on ZSM-5 Scaffold for bone tissue engineering applications. Materials, 15 (14). pp. 1-18. ISSN 1996-1944 http://dx.doi.org/10.3390/ma15144786 DOI:10.3390/ma15144786 |
| spellingShingle | TJ Mechanical engineering and machinery TP Chemical technology Aslani, Zahra Nazemi, N. Rajabi, Negar Kharaziha, Mahshid Bakhsheshi Rad, Hamid Reza Kasiri Asgarani, M. Najafinezhad, Aliakbar Ismail, Ahamd Fauzi Shari, S. Berto, Filippo Antibacterial activity and cell responses of vancomycin-loaded alginate coating on ZSM-5 Scaffold for bone tissue engineering applications |
| title | Antibacterial activity and cell responses of vancomycin-loaded alginate coating on ZSM-5 Scaffold for bone tissue engineering applications |
| title_full | Antibacterial activity and cell responses of vancomycin-loaded alginate coating on ZSM-5 Scaffold for bone tissue engineering applications |
| title_fullStr | Antibacterial activity and cell responses of vancomycin-loaded alginate coating on ZSM-5 Scaffold for bone tissue engineering applications |
| title_full_unstemmed | Antibacterial activity and cell responses of vancomycin-loaded alginate coating on ZSM-5 Scaffold for bone tissue engineering applications |
| title_short | Antibacterial activity and cell responses of vancomycin-loaded alginate coating on ZSM-5 Scaffold for bone tissue engineering applications |
| title_sort | antibacterial activity and cell responses of vancomycin loaded alginate coating on zsm 5 scaffold for bone tissue engineering applications |
| topic | TJ Mechanical engineering and machinery TP Chemical technology |
| url | http://eprints.utm.my/102934/1/AhamdFauziIsmail2022_AntibacterialActivityandCellResponses.pdf |
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