Production and characterization of a novel biosurfactant molecule from Bacillus safensis YKS2 and assessment of its efficiencies in wastewater treatment by a directed metagenomic approach
Biosurfactant is a biodegradation accelerator that improves bioavailability and facilitates degradation by microorganisms. The study was meant to produce a novel biosurfactant molecule from Bacillus safensis YKS2. An efficient biosurfactant-producing strain, namely, Bacillus safensis YKS2, was selec...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI
2022
|
| Subjects: | |
| Online Access: | http://eprints.utm.my/104308/1/ShreeshivadasanChelliapan2022_ProductionandCharacterizationofaNovelBiosurfactant.pdf |
| _version_ | 1796867631655944192 |
|---|---|
| author | Kalaimurugan, Dharman Balamuralikrishnan, Balasubramanian Govindarajan, Rasiravathanahalli Kaveriyappan Al-Dhabi, Naif Abdullah Arasu, Mariadhas Valan Vadivalagan, Chithravel Venkatesan, Srinivasan Kamyab, Hesam Chelliapan, Shreeshivadasan Khanongnuch, Chartchai |
| author_facet | Kalaimurugan, Dharman Balamuralikrishnan, Balasubramanian Govindarajan, Rasiravathanahalli Kaveriyappan Al-Dhabi, Naif Abdullah Arasu, Mariadhas Valan Vadivalagan, Chithravel Venkatesan, Srinivasan Kamyab, Hesam Chelliapan, Shreeshivadasan Khanongnuch, Chartchai |
| author_sort | Kalaimurugan, Dharman |
| collection | ePrints |
| description | Biosurfactant is a biodegradation accelerator that improves bioavailability and facilitates degradation by microorganisms. The study was meant to produce a novel biosurfactant molecule from Bacillus safensis YKS2. An efficient biosurfactant-producing strain, namely, Bacillus safensis YKS2, was selected using hemolytic activity, drop collapsing test, oil spreading test and blue agar plate methods in four oil-degrading strains isolated from a soil sample. Biosurfactant production in the optimization of bacteria culture conditions by RSM is a statistical grouping technique that is analyzed using the AVOVA approach to surface tention. In addition, the study was characterized by UV spectrophotometer FT-IR, HR-SEM, and GC-MS analyses to explain its structural and chemical details. Wastewater treatment was monitored for pH, EC, turbidity, alkalinity, chemical oxygen demand (COD), biochemical oxygen demand (BOD) and dissolved oxygen (DO) in order to justify the efficacy of the biosurfactant during wastewater treatment. The results of the UV spectrophotometer showed absorption at 530 nm, and the FT-IR analyzed carboxylic acids, alcohol and phenols groups, whichthe GC-MS analysis indicated were lipopeptide purified by hexadecanoic andtetradecanoic processes, respectively. The results show that the wastewater removal efficiency of 70% wasachieved within 24 h. In comparison, metagenomics was conducted during the treatment process to identify changes in the microbial load and diversity, which essentially indicatethe biosurfactant performance of the wastewater treatment process. The microbial load in the treated biosurfactant wastewater (84,374 sequences) was greatly decreased compared to untreated wastewater (139,568 sequences). It was concluded that B. safensis YKS2, producing a glycolipid form of biosurfactant, has possible benefits in the remediation of wastewater, and can be used for large-scale processing inbiosurfactant industries. |
| first_indexed | 2024-03-05T21:30:10Z |
| format | Article |
| id | utm.eprints-104308 |
| institution | Universiti Teknologi Malaysia - ePrints |
| language | English |
| last_indexed | 2024-03-05T21:30:10Z |
| publishDate | 2022 |
| publisher | MDPI |
| record_format | dspace |
| spelling | utm.eprints-1043082024-01-27T01:46:53Z http://eprints.utm.my/104308/ Production and characterization of a novel biosurfactant molecule from Bacillus safensis YKS2 and assessment of its efficiencies in wastewater treatment by a directed metagenomic approach Kalaimurugan, Dharman Balamuralikrishnan, Balasubramanian Govindarajan, Rasiravathanahalli Kaveriyappan Al-Dhabi, Naif Abdullah Arasu, Mariadhas Valan Vadivalagan, Chithravel Venkatesan, Srinivasan Kamyab, Hesam Chelliapan, Shreeshivadasan Khanongnuch, Chartchai TA Engineering (General). Civil engineering (General) Biosurfactant is a biodegradation accelerator that improves bioavailability and facilitates degradation by microorganisms. The study was meant to produce a novel biosurfactant molecule from Bacillus safensis YKS2. An efficient biosurfactant-producing strain, namely, Bacillus safensis YKS2, was selected using hemolytic activity, drop collapsing test, oil spreading test and blue agar plate methods in four oil-degrading strains isolated from a soil sample. Biosurfactant production in the optimization of bacteria culture conditions by RSM is a statistical grouping technique that is analyzed using the AVOVA approach to surface tention. In addition, the study was characterized by UV spectrophotometer FT-IR, HR-SEM, and GC-MS analyses to explain its structural and chemical details. Wastewater treatment was monitored for pH, EC, turbidity, alkalinity, chemical oxygen demand (COD), biochemical oxygen demand (BOD) and dissolved oxygen (DO) in order to justify the efficacy of the biosurfactant during wastewater treatment. The results of the UV spectrophotometer showed absorption at 530 nm, and the FT-IR analyzed carboxylic acids, alcohol and phenols groups, whichthe GC-MS analysis indicated were lipopeptide purified by hexadecanoic andtetradecanoic processes, respectively. The results show that the wastewater removal efficiency of 70% wasachieved within 24 h. In comparison, metagenomics was conducted during the treatment process to identify changes in the microbial load and diversity, which essentially indicatethe biosurfactant performance of the wastewater treatment process. The microbial load in the treated biosurfactant wastewater (84,374 sequences) was greatly decreased compared to untreated wastewater (139,568 sequences). It was concluded that B. safensis YKS2, producing a glycolipid form of biosurfactant, has possible benefits in the remediation of wastewater, and can be used for large-scale processing inbiosurfactant industries. MDPI 2022-02-02 Article PeerReviewed application/pdf en http://eprints.utm.my/104308/1/ShreeshivadasanChelliapan2022_ProductionandCharacterizationofaNovelBiosurfactant.pdf Kalaimurugan, Dharman and Balamuralikrishnan, Balasubramanian and Govindarajan, Rasiravathanahalli Kaveriyappan and Al-Dhabi, Naif Abdullah and Arasu, Mariadhas Valan and Vadivalagan, Chithravel and Venkatesan, Srinivasan and Kamyab, Hesam and Chelliapan, Shreeshivadasan and Khanongnuch, Chartchai (2022) Production and characterization of a novel biosurfactant molecule from Bacillus safensis YKS2 and assessment of its efficiencies in wastewater treatment by a directed metagenomic approach. Sustainability, 14 (4). pp. 1-20. ISSN 2071-1050 http://dx.doi.org/10.3390/su14042142 DOI:10.3390/su14042142 |
| spellingShingle | TA Engineering (General). Civil engineering (General) Kalaimurugan, Dharman Balamuralikrishnan, Balasubramanian Govindarajan, Rasiravathanahalli Kaveriyappan Al-Dhabi, Naif Abdullah Arasu, Mariadhas Valan Vadivalagan, Chithravel Venkatesan, Srinivasan Kamyab, Hesam Chelliapan, Shreeshivadasan Khanongnuch, Chartchai Production and characterization of a novel biosurfactant molecule from Bacillus safensis YKS2 and assessment of its efficiencies in wastewater treatment by a directed metagenomic approach |
| title | Production and characterization of a novel biosurfactant molecule from Bacillus safensis YKS2 and assessment of its efficiencies in wastewater treatment by a directed metagenomic approach |
| title_full | Production and characterization of a novel biosurfactant molecule from Bacillus safensis YKS2 and assessment of its efficiencies in wastewater treatment by a directed metagenomic approach |
| title_fullStr | Production and characterization of a novel biosurfactant molecule from Bacillus safensis YKS2 and assessment of its efficiencies in wastewater treatment by a directed metagenomic approach |
| title_full_unstemmed | Production and characterization of a novel biosurfactant molecule from Bacillus safensis YKS2 and assessment of its efficiencies in wastewater treatment by a directed metagenomic approach |
| title_short | Production and characterization of a novel biosurfactant molecule from Bacillus safensis YKS2 and assessment of its efficiencies in wastewater treatment by a directed metagenomic approach |
| title_sort | production and characterization of a novel biosurfactant molecule from bacillus safensis yks2 and assessment of its efficiencies in wastewater treatment by a directed metagenomic approach |
| topic | TA Engineering (General). Civil engineering (General) |
| url | http://eprints.utm.my/104308/1/ShreeshivadasanChelliapan2022_ProductionandCharacterizationofaNovelBiosurfactant.pdf |
| work_keys_str_mv | AT kalaimurugandharman productionandcharacterizationofanovelbiosurfactantmoleculefrombacillussafensisyks2andassessmentofitsefficienciesinwastewatertreatmentbyadirectedmetagenomicapproach AT balamuralikrishnanbalasubramanian productionandcharacterizationofanovelbiosurfactantmoleculefrombacillussafensisyks2andassessmentofitsefficienciesinwastewatertreatmentbyadirectedmetagenomicapproach AT govindarajanrasiravathanahallikaveriyappan productionandcharacterizationofanovelbiosurfactantmoleculefrombacillussafensisyks2andassessmentofitsefficienciesinwastewatertreatmentbyadirectedmetagenomicapproach AT aldhabinaifabdullah productionandcharacterizationofanovelbiosurfactantmoleculefrombacillussafensisyks2andassessmentofitsefficienciesinwastewatertreatmentbyadirectedmetagenomicapproach AT arasumariadhasvalan productionandcharacterizationofanovelbiosurfactantmoleculefrombacillussafensisyks2andassessmentofitsefficienciesinwastewatertreatmentbyadirectedmetagenomicapproach AT vadivalaganchithravel productionandcharacterizationofanovelbiosurfactantmoleculefrombacillussafensisyks2andassessmentofitsefficienciesinwastewatertreatmentbyadirectedmetagenomicapproach AT venkatesansrinivasan productionandcharacterizationofanovelbiosurfactantmoleculefrombacillussafensisyks2andassessmentofitsefficienciesinwastewatertreatmentbyadirectedmetagenomicapproach AT kamyabhesam productionandcharacterizationofanovelbiosurfactantmoleculefrombacillussafensisyks2andassessmentofitsefficienciesinwastewatertreatmentbyadirectedmetagenomicapproach AT chelliapanshreeshivadasan productionandcharacterizationofanovelbiosurfactantmoleculefrombacillussafensisyks2andassessmentofitsefficienciesinwastewatertreatmentbyadirectedmetagenomicapproach AT khanongnuchchartchai productionandcharacterizationofanovelbiosurfactantmoleculefrombacillussafensisyks2andassessmentofitsefficienciesinwastewatertreatmentbyadirectedmetagenomicapproach |