In-situ fabrication of functionalized starch magnetic nanoparticles for immobilization of laccase
Surface chemistry of magnetic nanoparticles (MNP) is crucial to provide a strong protein-support interaction for the immobilization process. The stability and biocompatibility of the MNP can be structurally enhanced by integrating with organic materials. In this study, MNP from KI/FeCl3 has successf...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit UTM Press
2023
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| Online Access: | http://eprints.utm.my/105050/1/NardiahRizwanaJaafar2023_InSituFabricationOfFunctionalizedStarch.pdf |
| _version_ | 1796867778710339584 |
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| author | Suhaimi, Suhaily Jaafar, Nardiah Rizwana Jailani, Nashriq A. Rahman, Roshanida Ngadi, Norzita Abdul Murad, Abdul Munir Hashim, Noor Haza Fazlin Md. Illias, Rosli |
| author_facet | Suhaimi, Suhaily Jaafar, Nardiah Rizwana Jailani, Nashriq A. Rahman, Roshanida Ngadi, Norzita Abdul Murad, Abdul Munir Hashim, Noor Haza Fazlin Md. Illias, Rosli |
| author_sort | Suhaimi, Suhaily |
| collection | ePrints |
| description | Surface chemistry of magnetic nanoparticles (MNP) is crucial to provide a strong protein-support interaction for the immobilization process. The stability and biocompatibility of the MNP can be structurally enhanced by integrating with organic materials. In this study, MNP from KI/FeCl3 has successfully synthesized that showed a stronger magnetic strength (72.5 emu/g) compared to common standard precursors, FeCl2/FeSO4 (< 60 emu/g). The synthesized MNP was then incorporated via in-situ with functionalized starch; dialdehyde (DAS-MNP), thiol (TS-MNP), and carboxymethyl (CMS-MNP) for Laccase (Lac) immobilization. From docking analysis, CMS-MNP portrayed the highest binding affinity and interacted with highest number of Lac amino acids residues compared to DAS-and TS-MNP. Aligned with this result, immobilized Lac using CMS-MNP achieved the highest recovery activity (80.3%), highly stable at 75 °C for 4 h, and retained more than 50% of its initial activity after 10 cycles. The CMS-MNP-Lac also showed about the same catalytic efficiency with free Lac (1.19 and 1.58 mM-1s-1, respectively). It is demonstrated that the functional group of the starch-MNP plays a crucial role in attaining a stable immobilized Lac. Therefore, yield a promising biocatalyst to be applied in various fields. |
| first_indexed | 2024-04-09T03:45:59Z |
| format | Article |
| id | utm.eprints-105050 |
| institution | Universiti Teknologi Malaysia - ePrints |
| language | English |
| last_indexed | 2024-04-09T03:45:59Z |
| publishDate | 2023 |
| publisher | Penerbit UTM Press |
| record_format | dspace |
| spelling | utm.eprints-1050502024-04-02T06:41:05Z http://eprints.utm.my/105050/ In-situ fabrication of functionalized starch magnetic nanoparticles for immobilization of laccase Suhaimi, Suhaily Jaafar, Nardiah Rizwana Jailani, Nashriq A. Rahman, Roshanida Ngadi, Norzita Abdul Murad, Abdul Munir Hashim, Noor Haza Fazlin Md. Illias, Rosli Q Science (General) Surface chemistry of magnetic nanoparticles (MNP) is crucial to provide a strong protein-support interaction for the immobilization process. The stability and biocompatibility of the MNP can be structurally enhanced by integrating with organic materials. In this study, MNP from KI/FeCl3 has successfully synthesized that showed a stronger magnetic strength (72.5 emu/g) compared to common standard precursors, FeCl2/FeSO4 (< 60 emu/g). The synthesized MNP was then incorporated via in-situ with functionalized starch; dialdehyde (DAS-MNP), thiol (TS-MNP), and carboxymethyl (CMS-MNP) for Laccase (Lac) immobilization. From docking analysis, CMS-MNP portrayed the highest binding affinity and interacted with highest number of Lac amino acids residues compared to DAS-and TS-MNP. Aligned with this result, immobilized Lac using CMS-MNP achieved the highest recovery activity (80.3%), highly stable at 75 °C for 4 h, and retained more than 50% of its initial activity after 10 cycles. The CMS-MNP-Lac also showed about the same catalytic efficiency with free Lac (1.19 and 1.58 mM-1s-1, respectively). It is demonstrated that the functional group of the starch-MNP plays a crucial role in attaining a stable immobilized Lac. Therefore, yield a promising biocatalyst to be applied in various fields. Penerbit UTM Press 2023-11 Article PeerReviewed application/pdf en http://eprints.utm.my/105050/1/NardiahRizwanaJaafar2023_InSituFabricationOfFunctionalizedStarch.pdf Suhaimi, Suhaily and Jaafar, Nardiah Rizwana and Jailani, Nashriq and A. Rahman, Roshanida and Ngadi, Norzita and Abdul Murad, Abdul Munir and Hashim, Noor Haza Fazlin and Md. Illias, Rosli (2023) In-situ fabrication of functionalized starch magnetic nanoparticles for immobilization of laccase. Jurnal Teknologi, 85 (6). pp. 169-179. ISSN 0127-9696 http://dx.doi.org/10.11113/jurnalteknologi.v85.20554 DOI:10.11113/jurnalteknologi.v85.20554 |
| spellingShingle | Q Science (General) Suhaimi, Suhaily Jaafar, Nardiah Rizwana Jailani, Nashriq A. Rahman, Roshanida Ngadi, Norzita Abdul Murad, Abdul Munir Hashim, Noor Haza Fazlin Md. Illias, Rosli In-situ fabrication of functionalized starch magnetic nanoparticles for immobilization of laccase |
| title | In-situ fabrication of functionalized starch magnetic nanoparticles for immobilization of laccase |
| title_full | In-situ fabrication of functionalized starch magnetic nanoparticles for immobilization of laccase |
| title_fullStr | In-situ fabrication of functionalized starch magnetic nanoparticles for immobilization of laccase |
| title_full_unstemmed | In-situ fabrication of functionalized starch magnetic nanoparticles for immobilization of laccase |
| title_short | In-situ fabrication of functionalized starch magnetic nanoparticles for immobilization of laccase |
| title_sort | in situ fabrication of functionalized starch magnetic nanoparticles for immobilization of laccase |
| topic | Q Science (General) |
| url | http://eprints.utm.my/105050/1/NardiahRizwanaJaafar2023_InSituFabricationOfFunctionalizedStarch.pdf |
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