Combined cross-linked enzyme aggregates of cyclodextrin glucanotransferase and maltogenic amylase from bacillus lehensis G1 for maltooligosaccharides synthesis.
Maltooligosaccharides (MOS) are functional oligosaccharides that can be synthesized through enzymatic cascade reaction between cyclodextrin glucanotransferase (CGTase) and maltogenic amylase (Mag1) from Bacillus lehensis G1. To address the problems of low operational stability and non-reusability of...
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Elsevier B.V.
2023
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| author | Yip, Yee Seng Abdul Manas, Nor Hasmaliana Jaafar, Nardiah Rizwana A. Rahman, Roshanida Puspaningsih, Ni Nyoman Tri Md. Illias, Rosli |
| author_facet | Yip, Yee Seng Abdul Manas, Nor Hasmaliana Jaafar, Nardiah Rizwana A. Rahman, Roshanida Puspaningsih, Ni Nyoman Tri Md. Illias, Rosli |
| author_sort | Yip, Yee Seng |
| collection | ePrints |
| description | Maltooligosaccharides (MOS) are functional oligosaccharides that can be synthesized through enzymatic cascade reaction between cyclodextrin glucanotransferase (CGTase) and maltogenic amylase (Mag1) from Bacillus lehensis G1. To address the problems of low operational stability and non-reusability of free enzymes, both enzymes were co-immobilized as combined cross-linked enzyme aggregates (Combi-CLEAs-CM) with incorporation of bovine serum albumin (BSA) and Tween 80 (Combi-CLEAs-CM-add). Combi-CLEAs-CM and Combi-CLEAs-CM-add showed activity recoveries of 54.12 % and 69.44 %, respectively after optimization. Combi-CLEAs-CM-add showed higher thermal stability at higher temperatures (40 °C) with longer half-life (46.20 min) as compared to those of free enzymes (36.67 min) and Combi-CLEAs-CM (41.51 min). Both combi-CLEAs also exhibited higher pH stability over pH 5 to pH 9, and displayed excellent reusability with >50 % of initial activity retained after four cycles. The reduction in Km value of about 22.80 % and 1.76-fold increase in starch hydrolysis in comparison to Combi-CLEAs-CM attested the improvement of enzyme-substrate interaction by Tween 80 and pores formation by BSA in Combi-CLEAs-CM-add. The improved product specificity of Combi-CLEAs-CM-add also produced the highest yield of MOS (492 mg/g) after 3 h. Therefore, Combi-CLEAs-CM-add with ease of preparation, excellent reusability and high operational stability is believed to be highly efficacious biocatalyst for MOS production. |
| first_indexed | 2024-09-23T23:57:30Z |
| format | Article |
| id | utm.eprints-105468 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-09-23T23:57:30Z |
| publishDate | 2023 |
| publisher | Elsevier B.V. |
| record_format | dspace |
| spelling | utm.eprints-1054682024-05-02T02:52:31Z http://eprints.utm.my/105468/ Combined cross-linked enzyme aggregates of cyclodextrin glucanotransferase and maltogenic amylase from bacillus lehensis G1 for maltooligosaccharides synthesis. Yip, Yee Seng Abdul Manas, Nor Hasmaliana Jaafar, Nardiah Rizwana A. Rahman, Roshanida Puspaningsih, Ni Nyoman Tri Md. Illias, Rosli Q Science (General) QD Chemistry Maltooligosaccharides (MOS) are functional oligosaccharides that can be synthesized through enzymatic cascade reaction between cyclodextrin glucanotransferase (CGTase) and maltogenic amylase (Mag1) from Bacillus lehensis G1. To address the problems of low operational stability and non-reusability of free enzymes, both enzymes were co-immobilized as combined cross-linked enzyme aggregates (Combi-CLEAs-CM) with incorporation of bovine serum albumin (BSA) and Tween 80 (Combi-CLEAs-CM-add). Combi-CLEAs-CM and Combi-CLEAs-CM-add showed activity recoveries of 54.12 % and 69.44 %, respectively after optimization. Combi-CLEAs-CM-add showed higher thermal stability at higher temperatures (40 °C) with longer half-life (46.20 min) as compared to those of free enzymes (36.67 min) and Combi-CLEAs-CM (41.51 min). Both combi-CLEAs also exhibited higher pH stability over pH 5 to pH 9, and displayed excellent reusability with >50 % of initial activity retained after four cycles. The reduction in Km value of about 22.80 % and 1.76-fold increase in starch hydrolysis in comparison to Combi-CLEAs-CM attested the improvement of enzyme-substrate interaction by Tween 80 and pores formation by BSA in Combi-CLEAs-CM-add. The improved product specificity of Combi-CLEAs-CM-add also produced the highest yield of MOS (492 mg/g) after 3 h. Therefore, Combi-CLEAs-CM-add with ease of preparation, excellent reusability and high operational stability is believed to be highly efficacious biocatalyst for MOS production. Elsevier B.V. 2023-07-01 Article PeerReviewed Yip, Yee Seng and Abdul Manas, Nor Hasmaliana and Jaafar, Nardiah Rizwana and A. Rahman, Roshanida and Puspaningsih, Ni Nyoman Tri and Md. Illias, Rosli (2023) Combined cross-linked enzyme aggregates of cyclodextrin glucanotransferase and maltogenic amylase from bacillus lehensis G1 for maltooligosaccharides synthesis. International Journal of Biological Macromolecules, 242 (124675). NA-NA. ISSN 0141-8130 http://dx.doi.org/10.1016/j.ijbiomac.2023.124675 DOI: 10.1016/j.ijbiomac.2023.124675 |
| spellingShingle | Q Science (General) QD Chemistry Yip, Yee Seng Abdul Manas, Nor Hasmaliana Jaafar, Nardiah Rizwana A. Rahman, Roshanida Puspaningsih, Ni Nyoman Tri Md. Illias, Rosli Combined cross-linked enzyme aggregates of cyclodextrin glucanotransferase and maltogenic amylase from bacillus lehensis G1 for maltooligosaccharides synthesis. |
| title | Combined cross-linked enzyme aggregates of cyclodextrin glucanotransferase and maltogenic amylase from bacillus lehensis G1 for maltooligosaccharides synthesis. |
| title_full | Combined cross-linked enzyme aggregates of cyclodextrin glucanotransferase and maltogenic amylase from bacillus lehensis G1 for maltooligosaccharides synthesis. |
| title_fullStr | Combined cross-linked enzyme aggregates of cyclodextrin glucanotransferase and maltogenic amylase from bacillus lehensis G1 for maltooligosaccharides synthesis. |
| title_full_unstemmed | Combined cross-linked enzyme aggregates of cyclodextrin glucanotransferase and maltogenic amylase from bacillus lehensis G1 for maltooligosaccharides synthesis. |
| title_short | Combined cross-linked enzyme aggregates of cyclodextrin glucanotransferase and maltogenic amylase from bacillus lehensis G1 for maltooligosaccharides synthesis. |
| title_sort | combined cross linked enzyme aggregates of cyclodextrin glucanotransferase and maltogenic amylase from bacillus lehensis g1 for maltooligosaccharides synthesis |
| topic | Q Science (General) QD Chemistry |
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