Investigation through the antimicrobial activity Of electrospun Pcl nanofiber mats with green synthesized Ag-Fe nanoparticles.
Wound or device infections with drug resistant microorganisms are a major challenge in medicine that could increase morbidity and mortality rate, with a great financial burden on the health care system. Nanofibers with antimicrobial coating have been widely applied in wound dressing and tissue engin...
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| Format: | Article |
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Editions de Sante
2023
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| author | Jadidi Kouhbanani, Mohammad Amin Mosleh-Shirazi, Sareh Beheshtkhoo, Nasrin Kasaee, Seyed Reza Nekouian, Soraya Alshehery, Sultan Kamyab, Hesam Chelliapan, Shreeshivadasan Ali, Mohammed Azam Amani, Ali Mohammad |
| author_facet | Jadidi Kouhbanani, Mohammad Amin Mosleh-Shirazi, Sareh Beheshtkhoo, Nasrin Kasaee, Seyed Reza Nekouian, Soraya Alshehery, Sultan Kamyab, Hesam Chelliapan, Shreeshivadasan Ali, Mohammed Azam Amani, Ali Mohammad |
| author_sort | Jadidi Kouhbanani, Mohammad Amin |
| collection | ePrints |
| description | Wound or device infections with drug resistant microorganisms are a major challenge in medicine that could increase morbidity and mortality rate, with a great financial burden on the health care system. Nanofibers with antimicrobial coating have been widely applied in wound dressing and tissue engineering to decrease the risk of infections. In the present study, Ag–Fe core-shell NPs were green synthesized by applying Ducrosia anethifolia plant extracted as a stabilizing and reducing agent. The novel nanocomposites by a combination of Ag–Fe and polymeric polycaprolactone (PCL) nanofibers were produced. Synthesized nanoparticles and nanocomposites were characterized using several physical and mechanical techniques, including scanning electron microscopy (SEM), dynamic light scattering method (DLS), X-ray diffraction (XRD), transmission electron microscopy (TEM), fourier transform infrared (FT-IR) spectroscopy, ultraviolet–visible absorption spectroscopy (UV–vis), and tensile test. The mean size of the synthesized Ag and Ag–Fe core-shell nanoparticles were 4.1 and 18.4 nm. Silver nanoparticles were successfully coated with Fe shell and nearly spherical core-shell shape. SEM images showed that PCL nanofibers have an average size of 265 nm. The results revealed that the increase in the Ag–Fe core-shell NPs' concentration leads to the smaller size of nanofibers. The antimicrobial properties of Ag–Fe core-shell and PCL/Ag–Fe nanocomposites were evaluated against some clinically important bacteria and fungi. Also, the cytotoxicity of PCL/Ag–Fe nanocomposites on fibroblast cells Natural man (NHDF) was assessed for 24, 48, and 72 h. The results of the study confirmed minimal cellular toxicity and considerable antimicrobial properties of green synthesized PCL/Ag–Fe nanocomposites, especially against some drug resistant pathogens, which could be promising new insight to improve the PCL-based composites applications in wound dressings or medical devices. |
| first_indexed | 2024-09-24T00:02:19Z |
| format | Article |
| id | utm.eprints-106468 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-09-24T00:02:19Z |
| publishDate | 2023 |
| publisher | Editions de Sante |
| record_format | dspace |
| spelling | utm.eprints-1064682024-07-08T07:29:42Z http://eprints.utm.my/106468/ Investigation through the antimicrobial activity Of electrospun Pcl nanofiber mats with green synthesized Ag-Fe nanoparticles. Jadidi Kouhbanani, Mohammad Amin Mosleh-Shirazi, Sareh Beheshtkhoo, Nasrin Kasaee, Seyed Reza Nekouian, Soraya Alshehery, Sultan Kamyab, Hesam Chelliapan, Shreeshivadasan Ali, Mohammed Azam Amani, Ali Mohammad TP Chemical technology Wound or device infections with drug resistant microorganisms are a major challenge in medicine that could increase morbidity and mortality rate, with a great financial burden on the health care system. Nanofibers with antimicrobial coating have been widely applied in wound dressing and tissue engineering to decrease the risk of infections. In the present study, Ag–Fe core-shell NPs were green synthesized by applying Ducrosia anethifolia plant extracted as a stabilizing and reducing agent. The novel nanocomposites by a combination of Ag–Fe and polymeric polycaprolactone (PCL) nanofibers were produced. Synthesized nanoparticles and nanocomposites were characterized using several physical and mechanical techniques, including scanning electron microscopy (SEM), dynamic light scattering method (DLS), X-ray diffraction (XRD), transmission electron microscopy (TEM), fourier transform infrared (FT-IR) spectroscopy, ultraviolet–visible absorption spectroscopy (UV–vis), and tensile test. The mean size of the synthesized Ag and Ag–Fe core-shell nanoparticles were 4.1 and 18.4 nm. Silver nanoparticles were successfully coated with Fe shell and nearly spherical core-shell shape. SEM images showed that PCL nanofibers have an average size of 265 nm. The results revealed that the increase in the Ag–Fe core-shell NPs' concentration leads to the smaller size of nanofibers. The antimicrobial properties of Ag–Fe core-shell and PCL/Ag–Fe nanocomposites were evaluated against some clinically important bacteria and fungi. Also, the cytotoxicity of PCL/Ag–Fe nanocomposites on fibroblast cells Natural man (NHDF) was assessed for 24, 48, and 72 h. The results of the study confirmed minimal cellular toxicity and considerable antimicrobial properties of green synthesized PCL/Ag–Fe nanocomposites, especially against some drug resistant pathogens, which could be promising new insight to improve the PCL-based composites applications in wound dressings or medical devices. Editions de Sante 2023-08 Article PeerReviewed Jadidi Kouhbanani, Mohammad Amin and Mosleh-Shirazi, Sareh and Beheshtkhoo, Nasrin and Kasaee, Seyed Reza and Nekouian, Soraya and Alshehery, Sultan and Kamyab, Hesam and Chelliapan, Shreeshivadasan and Ali, Mohammed Azam and Amani, Ali Mohammad (2023) Investigation through the antimicrobial activity Of electrospun Pcl nanofiber mats with green synthesized Ag-Fe nanoparticles. Journal of Drug Delivery Science and Technology, 85 (104541). NA-NA. ISSN 1773-2247 http://dx.doi.org/10.1016/j.jddst.2023.104541 DOI: 10.1016/j.jddst.2023.104541 |
| spellingShingle | TP Chemical technology Jadidi Kouhbanani, Mohammad Amin Mosleh-Shirazi, Sareh Beheshtkhoo, Nasrin Kasaee, Seyed Reza Nekouian, Soraya Alshehery, Sultan Kamyab, Hesam Chelliapan, Shreeshivadasan Ali, Mohammed Azam Amani, Ali Mohammad Investigation through the antimicrobial activity Of electrospun Pcl nanofiber mats with green synthesized Ag-Fe nanoparticles. |
| title | Investigation through the antimicrobial activity Of electrospun Pcl nanofiber mats with green synthesized Ag-Fe nanoparticles. |
| title_full | Investigation through the antimicrobial activity Of electrospun Pcl nanofiber mats with green synthesized Ag-Fe nanoparticles. |
| title_fullStr | Investigation through the antimicrobial activity Of electrospun Pcl nanofiber mats with green synthesized Ag-Fe nanoparticles. |
| title_full_unstemmed | Investigation through the antimicrobial activity Of electrospun Pcl nanofiber mats with green synthesized Ag-Fe nanoparticles. |
| title_short | Investigation through the antimicrobial activity Of electrospun Pcl nanofiber mats with green synthesized Ag-Fe nanoparticles. |
| title_sort | investigation through the antimicrobial activity of electrospun pcl nanofiber mats with green synthesized ag fe nanoparticles |
| topic | TP Chemical technology |
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