Optimization of ultrasound-assisted extraction for antioxidant activity in relation to rhoifolin content of Fortunella polyandra using response surface methodology (RSM)
Fortunella polyandra (Ridl) Tanaka or Malayan kumquat, is a family of Rutaceae from Malaysia known for its various active compounds, especially flavonoids. This study aims to verify the optimum condition and correlation of leaves extract in Fortunella polyandra by ultrasound-assisted extraction (UAE...
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| Format: | Article |
| Language: | English |
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Penerbit UKM
2023
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| Online Access: | http://eprints.utm.my/106726/1/NorazahBasar2023_OptimizationofUltrasoundAssistedExtractionforAntioxidant.pdf |
| _version_ | 1811132399949447168 |
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| author | Elias, Hazim Syahmi Baharum, Syarul Nataqain Azizan, Kamalrul Azlan Wan Ahmad, Wan Yaacob Chandren, Sheela Basar, Norazah |
| author_facet | Elias, Hazim Syahmi Baharum, Syarul Nataqain Azizan, Kamalrul Azlan Wan Ahmad, Wan Yaacob Chandren, Sheela Basar, Norazah |
| author_sort | Elias, Hazim Syahmi |
| collection | ePrints |
| description | Fortunella polyandra (Ridl) Tanaka or Malayan kumquat, is a family of Rutaceae from Malaysia known for its various active compounds, especially flavonoids. This study aims to verify the optimum condition and correlation of leaves extract in Fortunella polyandra by ultrasound-assisted extraction (UAE) for antioxidant activities and apigenin 7-O-neohesperidoside (rhoifolin) contents using the response surface methodology (RSM). Central composite rotatable design (CCD) was performed to investigate the influence of the extraction parameters on maximum antioxidant activity, namely temperatures (X1), times (X2), and solvent ratios of ethanol:water (X3). The optimal extraction conditions for 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity were obtained at extraction time of 5 min, temperature of 30°C and solvent ratio of 70:30, ethanol:water with IC50 value of 0.126±0.004 mg/ mL. Rhoifolin content and ferric reducing antioxidant potential (FRAP) assay share the same optimum condition at extraction time of 5 min, temperature of 30°C, and solvent ratio of 30:70, ethanol:water. Under these conditions, rhoifolin content was measured at 67.83±0.37 ppm, whereas FRAP equivalent value was recorded at 0.22±0.01 mg/ mL. All three responses from the model achieved 95% confidence level. Correlation study showed that there is no relationship between the rhoifolin content and the antioxidant activities of Fortunella polyandra extract. |
| first_indexed | 2024-09-24T00:03:27Z |
| format | Article |
| id | utm.eprints-106726 |
| institution | Universiti Teknologi Malaysia - ePrints |
| language | English |
| last_indexed | 2024-09-24T00:03:27Z |
| publishDate | 2023 |
| publisher | Penerbit UKM |
| record_format | dspace |
| spelling | utm.eprints-1067262024-07-28T06:08:19Z http://eprints.utm.my/106726/ Optimization of ultrasound-assisted extraction for antioxidant activity in relation to rhoifolin content of Fortunella polyandra using response surface methodology (RSM) Elias, Hazim Syahmi Baharum, Syarul Nataqain Azizan, Kamalrul Azlan Wan Ahmad, Wan Yaacob Chandren, Sheela Basar, Norazah QD Chemistry Fortunella polyandra (Ridl) Tanaka or Malayan kumquat, is a family of Rutaceae from Malaysia known for its various active compounds, especially flavonoids. This study aims to verify the optimum condition and correlation of leaves extract in Fortunella polyandra by ultrasound-assisted extraction (UAE) for antioxidant activities and apigenin 7-O-neohesperidoside (rhoifolin) contents using the response surface methodology (RSM). Central composite rotatable design (CCD) was performed to investigate the influence of the extraction parameters on maximum antioxidant activity, namely temperatures (X1), times (X2), and solvent ratios of ethanol:water (X3). The optimal extraction conditions for 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity were obtained at extraction time of 5 min, temperature of 30°C and solvent ratio of 70:30, ethanol:water with IC50 value of 0.126±0.004 mg/ mL. Rhoifolin content and ferric reducing antioxidant potential (FRAP) assay share the same optimum condition at extraction time of 5 min, temperature of 30°C, and solvent ratio of 30:70, ethanol:water. Under these conditions, rhoifolin content was measured at 67.83±0.37 ppm, whereas FRAP equivalent value was recorded at 0.22±0.01 mg/ mL. All three responses from the model achieved 95% confidence level. Correlation study showed that there is no relationship between the rhoifolin content and the antioxidant activities of Fortunella polyandra extract. Penerbit UKM 2023-01 Article PeerReviewed application/pdf en http://eprints.utm.my/106726/1/NorazahBasar2023_OptimizationofUltrasoundAssistedExtractionforAntioxidant.pdf Elias, Hazim Syahmi and Baharum, Syarul Nataqain and Azizan, Kamalrul Azlan and Wan Ahmad, Wan Yaacob and Chandren, Sheela and Basar, Norazah (2023) Optimization of ultrasound-assisted extraction for antioxidant activity in relation to rhoifolin content of Fortunella polyandra using response surface methodology (RSM). Sains Malaysiana, 52 (1). pp. 153-164. ISSN 0126-6039 http://dx.doi.org/10.17576/jsm-2023-5201-12 DOI:10.17576/jsm-2023-5201-12 |
| spellingShingle | QD Chemistry Elias, Hazim Syahmi Baharum, Syarul Nataqain Azizan, Kamalrul Azlan Wan Ahmad, Wan Yaacob Chandren, Sheela Basar, Norazah Optimization of ultrasound-assisted extraction for antioxidant activity in relation to rhoifolin content of Fortunella polyandra using response surface methodology (RSM) |
| title | Optimization of ultrasound-assisted extraction for antioxidant activity in relation to rhoifolin content of Fortunella polyandra using response surface methodology (RSM) |
| title_full | Optimization of ultrasound-assisted extraction for antioxidant activity in relation to rhoifolin content of Fortunella polyandra using response surface methodology (RSM) |
| title_fullStr | Optimization of ultrasound-assisted extraction for antioxidant activity in relation to rhoifolin content of Fortunella polyandra using response surface methodology (RSM) |
| title_full_unstemmed | Optimization of ultrasound-assisted extraction for antioxidant activity in relation to rhoifolin content of Fortunella polyandra using response surface methodology (RSM) |
| title_short | Optimization of ultrasound-assisted extraction for antioxidant activity in relation to rhoifolin content of Fortunella polyandra using response surface methodology (RSM) |
| title_sort | optimization of ultrasound assisted extraction for antioxidant activity in relation to rhoifolin content of fortunella polyandra using response surface methodology rsm |
| topic | QD Chemistry |
| url | http://eprints.utm.my/106726/1/NorazahBasar2023_OptimizationofUltrasoundAssistedExtractionforAntioxidant.pdf |
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