Reassortment and genomic analysis of a G9P[8]-E2 rotavirus isolated in China
Objective: To isolate a prevalent G9P[8] group A rotavirus (RVA) (N4006) in China and investigate its genomic and evolutionary characteristics, with the goal of facilitating the development of a new rotavirus vaccine. Methods: The RVA G9P[8] genotype from a diarrhea sample was passaged in MA104 cell...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BioMed Central Ltd
2023
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| Online Access: | http://eprints.utm.my/107528/1/SalehaShahar2023_ReassortmentandGenomicAnalysisofaG9P8E2.pdf |
| _version_ | 1811132556007964672 |
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| author | Peng, Rui Li, Dandi Wang, Jindong Xiong, Guangping Wang, Mengxuan Liu, Dan Wei, Yuhang Pang, Lili Sun, Xiaoman Li, Huiying Kong, Xiangyu Shahar, Saleha Duan, Zhaojun |
| author_facet | Peng, Rui Li, Dandi Wang, Jindong Xiong, Guangping Wang, Mengxuan Liu, Dan Wei, Yuhang Pang, Lili Sun, Xiaoman Li, Huiying Kong, Xiangyu Shahar, Saleha Duan, Zhaojun |
| author_sort | Peng, Rui |
| collection | ePrints |
| description | Objective: To isolate a prevalent G9P[8] group A rotavirus (RVA) (N4006) in China and investigate its genomic and evolutionary characteristics, with the goal of facilitating the development of a new rotavirus vaccine. Methods: The RVA G9P[8] genotype from a diarrhea sample was passaged in MA104 cells. The virus was evaluated by TEM, polyacrylamide gel electrophoresis, and indirect immunofluorescence assay. The complete genome of virus was obtained by RT-PCR and sequencing. The genomic and evolutionary characteristics of the virus were evaluated by nucleic acid sequence analysis with MEGA ver. 5.0.5 and DNASTAR software. The neutralizing epitopes of VP7 and VP4 (VP5* and VP8*) were analyzed using BioEdit ver. 7.0.9.0 and PyMOL ver. 2.5.2. Results: The RVA N4006 (G9P[8] genotype) was adapted in MA104 cells with a high titer (105.5 PFU/mL). Whole-genome sequence analysis showed N4006 to be a reassortant rotavirus of Wa-like G9P[8] RVA and the NSP4 gene of DS-1-like G2P[4] RVA, with the genotype constellation G9-P[8]-I1-R1-C1-M1-A1-N1-T1-E2-H1 (G9P[8]-E2). Phylogenetic analysis indicated that N4006 had a common ancestor with Japanese G9P[8]-E2 rotavirus. Neutralizing epitope analysis showed that VP7, VP5*, and VP8* of N4006 had low homology with vaccine viruses of the same genotype and marked differences with vaccine viruses of other genotypes. Conclusion: The RVA G9P[8] genotype with the G9-P[8]-I1-R1-C1-M1-A1-N1-T1-E2-H1 (G9P[8]-E2) constellation predominates in China and may originate from reassortment between Japanese G9P[8] with Japanese DS-1-like G2P[4] rotaviruses. The antigenic variation of N4006 with the vaccine virus necessitates an evaluation of the effect of the rotavirus vaccine on G9P[8]-E2 genotype rotavirus. |
| first_indexed | 2024-09-24T00:06:12Z |
| format | Article |
| id | utm.eprints-107528 |
| institution | Universiti Teknologi Malaysia - ePrints |
| language | English |
| last_indexed | 2024-09-24T00:06:12Z |
| publishDate | 2023 |
| publisher | BioMed Central Ltd |
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| spelling | utm.eprints-1075282024-09-23T04:21:58Z http://eprints.utm.my/107528/ Reassortment and genomic analysis of a G9P[8]-E2 rotavirus isolated in China Peng, Rui Li, Dandi Wang, Jindong Xiong, Guangping Wang, Mengxuan Liu, Dan Wei, Yuhang Pang, Lili Sun, Xiaoman Li, Huiying Kong, Xiangyu Shahar, Saleha Duan, Zhaojun Q Science (General) Objective: To isolate a prevalent G9P[8] group A rotavirus (RVA) (N4006) in China and investigate its genomic and evolutionary characteristics, with the goal of facilitating the development of a new rotavirus vaccine. Methods: The RVA G9P[8] genotype from a diarrhea sample was passaged in MA104 cells. The virus was evaluated by TEM, polyacrylamide gel electrophoresis, and indirect immunofluorescence assay. The complete genome of virus was obtained by RT-PCR and sequencing. The genomic and evolutionary characteristics of the virus were evaluated by nucleic acid sequence analysis with MEGA ver. 5.0.5 and DNASTAR software. The neutralizing epitopes of VP7 and VP4 (VP5* and VP8*) were analyzed using BioEdit ver. 7.0.9.0 and PyMOL ver. 2.5.2. Results: The RVA N4006 (G9P[8] genotype) was adapted in MA104 cells with a high titer (105.5 PFU/mL). Whole-genome sequence analysis showed N4006 to be a reassortant rotavirus of Wa-like G9P[8] RVA and the NSP4 gene of DS-1-like G2P[4] RVA, with the genotype constellation G9-P[8]-I1-R1-C1-M1-A1-N1-T1-E2-H1 (G9P[8]-E2). Phylogenetic analysis indicated that N4006 had a common ancestor with Japanese G9P[8]-E2 rotavirus. Neutralizing epitope analysis showed that VP7, VP5*, and VP8* of N4006 had low homology with vaccine viruses of the same genotype and marked differences with vaccine viruses of other genotypes. Conclusion: The RVA G9P[8] genotype with the G9-P[8]-I1-R1-C1-M1-A1-N1-T1-E2-H1 (G9P[8]-E2) constellation predominates in China and may originate from reassortment between Japanese G9P[8] with Japanese DS-1-like G2P[4] rotaviruses. The antigenic variation of N4006 with the vaccine virus necessitates an evaluation of the effect of the rotavirus vaccine on G9P[8]-E2 genotype rotavirus. BioMed Central Ltd 2023-06-22 Article PeerReviewed application/pdf en http://eprints.utm.my/107528/1/SalehaShahar2023_ReassortmentandGenomicAnalysisofaG9P8E2.pdf Peng, Rui and Li, Dandi and Wang, Jindong and Xiong, Guangping and Wang, Mengxuan and Liu, Dan and Wei, Yuhang and Pang, Lili and Sun, Xiaoman and Li, Huiying and Kong, Xiangyu and Shahar, Saleha and Duan, Zhaojun (2023) Reassortment and genomic analysis of a G9P[8]-E2 rotavirus isolated in China. Virology Journal, 20 (1). pp. 1-15. ISSN 1743-422X http://dx.doi.org/10.1186/s12985-023-02064-5 DOI:10.1186/s12985-023-02064-5 |
| spellingShingle | Q Science (General) Peng, Rui Li, Dandi Wang, Jindong Xiong, Guangping Wang, Mengxuan Liu, Dan Wei, Yuhang Pang, Lili Sun, Xiaoman Li, Huiying Kong, Xiangyu Shahar, Saleha Duan, Zhaojun Reassortment and genomic analysis of a G9P[8]-E2 rotavirus isolated in China |
| title | Reassortment and genomic analysis of a G9P[8]-E2 rotavirus isolated in China |
| title_full | Reassortment and genomic analysis of a G9P[8]-E2 rotavirus isolated in China |
| title_fullStr | Reassortment and genomic analysis of a G9P[8]-E2 rotavirus isolated in China |
| title_full_unstemmed | Reassortment and genomic analysis of a G9P[8]-E2 rotavirus isolated in China |
| title_short | Reassortment and genomic analysis of a G9P[8]-E2 rotavirus isolated in China |
| title_sort | reassortment and genomic analysis of a g9p 8 e2 rotavirus isolated in china |
| topic | Q Science (General) |
| url | http://eprints.utm.my/107528/1/SalehaShahar2023_ReassortmentandGenomicAnalysisofaG9P8E2.pdf |
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