Potential of Burkholderia sp. IMCC1007 as a biodetoxification agent in mycotoxin biotransformation evaluated by mass spectrometry and phytotoxicity analysis
Microbial degradation is considered as an attractive method to eliminate exposure to mycotoxin that cause a serious threat in agriculture global industry and severe human health problems. Compared with other more prominent mycotoxin compounds, fusaric acid (FA) biodegradation has not been widely inv...
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Springer Nature
2023
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| author | Mohd. Din, Abd. Rahman Jabir Shadan, Nur Hidayah Rosli, Mohamad Azzuan Musa, Nur Fashya Othman, Nor Zalina |
| author_facet | Mohd. Din, Abd. Rahman Jabir Shadan, Nur Hidayah Rosli, Mohamad Azzuan Musa, Nur Fashya Othman, Nor Zalina |
| author_sort | Mohd. Din, Abd. Rahman Jabir |
| collection | ePrints |
| description | Microbial degradation is considered as an attractive method to eliminate exposure to mycotoxin that cause a serious threat in agriculture global industry and severe human health problems. Compared with other more prominent mycotoxin compounds, fusaric acid (FA) biodegradation has not been widely investigated. In this study, a fusaric acid-degrading bacterium Burkholderia sp. IMCC1007 was identified by 16 S rRNA gene sequencing and its detoxification characteristics were evaluated. This strain able to utilize FA as sole energy and carbon source with growth rate (µ) of 0.18 h− 1. Approximately 93% from the initial substrate FA concentration was almost degraded to the residual about 4.87 mg L− 1 after 12 h of incubation. The optimal degradation conditions for pH and temperature were recorded at 6.0 with 30 °C respectively. An efficient FA degradation of strain IMCC1007 suggested its potential significance to detoxification development. Accroding to LC-MS/Q-TOF analysis, FA was bio-transformed to 4-hydroxybenzoic acid (C7H6O3) and other possible metabolites. Plant treated with detoxified FA products exhibited reduction of wilting index, mitigating against FA phytoxicity effect on plant growth and photosynthesis activity. Phytotoxicity bioassay suggested that degradation product of IMCC1007 was not a potent harmful compound towards plants as compared to the parent compound, FA. As a conslusion, our study provides a new insight into the practical application of biodetoxifcation agent in controlling mycotoxin contamination. |
| first_indexed | 2024-09-24T00:06:21Z |
| format | Article |
| id | utm.eprints-107604 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-09-24T00:06:21Z |
| publishDate | 2023 |
| publisher | Springer Nature |
| record_format | dspace |
| spelling | utm.eprints-1076042024-09-23T06:22:04Z http://eprints.utm.my/107604/ Potential of Burkholderia sp. IMCC1007 as a biodetoxification agent in mycotoxin biotransformation evaluated by mass spectrometry and phytotoxicity analysis Mohd. Din, Abd. Rahman Jabir Shadan, Nur Hidayah Rosli, Mohamad Azzuan Musa, Nur Fashya Othman, Nor Zalina Q Science (General) Microbial degradation is considered as an attractive method to eliminate exposure to mycotoxin that cause a serious threat in agriculture global industry and severe human health problems. Compared with other more prominent mycotoxin compounds, fusaric acid (FA) biodegradation has not been widely investigated. In this study, a fusaric acid-degrading bacterium Burkholderia sp. IMCC1007 was identified by 16 S rRNA gene sequencing and its detoxification characteristics were evaluated. This strain able to utilize FA as sole energy and carbon source with growth rate (µ) of 0.18 h− 1. Approximately 93% from the initial substrate FA concentration was almost degraded to the residual about 4.87 mg L− 1 after 12 h of incubation. The optimal degradation conditions for pH and temperature were recorded at 6.0 with 30 °C respectively. An efficient FA degradation of strain IMCC1007 suggested its potential significance to detoxification development. Accroding to LC-MS/Q-TOF analysis, FA was bio-transformed to 4-hydroxybenzoic acid (C7H6O3) and other possible metabolites. Plant treated with detoxified FA products exhibited reduction of wilting index, mitigating against FA phytoxicity effect on plant growth and photosynthesis activity. Phytotoxicity bioassay suggested that degradation product of IMCC1007 was not a potent harmful compound towards plants as compared to the parent compound, FA. As a conslusion, our study provides a new insight into the practical application of biodetoxifcation agent in controlling mycotoxin contamination. Springer Nature 2023 Article PeerReviewed Mohd. Din, Abd. Rahman Jabir and Shadan, Nur Hidayah and Rosli, Mohamad Azzuan and Musa, Nur Fashya and Othman, Nor Zalina (2023) Potential of Burkholderia sp. IMCC1007 as a biodetoxification agent in mycotoxin biotransformation evaluated by mass spectrometry and phytotoxicity analysis. World Journal of Microbiology and Biotechnology, 39 (4). NA. ISSN 0959-3993 http://dx.doi.org/10.1007/s11274-023-03544-0 DOI:10.1007/s11274-023-03544-0 |
| spellingShingle | Q Science (General) Mohd. Din, Abd. Rahman Jabir Shadan, Nur Hidayah Rosli, Mohamad Azzuan Musa, Nur Fashya Othman, Nor Zalina Potential of Burkholderia sp. IMCC1007 as a biodetoxification agent in mycotoxin biotransformation evaluated by mass spectrometry and phytotoxicity analysis |
| title | Potential of Burkholderia sp. IMCC1007 as a biodetoxification agent in mycotoxin biotransformation evaluated by mass spectrometry and phytotoxicity analysis |
| title_full | Potential of Burkholderia sp. IMCC1007 as a biodetoxification agent in mycotoxin biotransformation evaluated by mass spectrometry and phytotoxicity analysis |
| title_fullStr | Potential of Burkholderia sp. IMCC1007 as a biodetoxification agent in mycotoxin biotransformation evaluated by mass spectrometry and phytotoxicity analysis |
| title_full_unstemmed | Potential of Burkholderia sp. IMCC1007 as a biodetoxification agent in mycotoxin biotransformation evaluated by mass spectrometry and phytotoxicity analysis |
| title_short | Potential of Burkholderia sp. IMCC1007 as a biodetoxification agent in mycotoxin biotransformation evaluated by mass spectrometry and phytotoxicity analysis |
| title_sort | potential of burkholderia sp imcc1007 as a biodetoxification agent in mycotoxin biotransformation evaluated by mass spectrometry and phytotoxicity analysis |
| topic | Q Science (General) |
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