Primer concentration and pre-denaturation time effect on cyt-K bacillus cereus detection using real-time PCR method.
Foodborne disease is a global threat that can affect all sections of society, both in developed or developing countries. Bacillus cereus is a Gram-positive bacteria that can cause food poisoning disease in humans. [2] Real-Time PCR detection method is one of the molecular marker methods that has bee...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2023
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| Subjects: | |
| Online Access: | http://eprints.utm.my/107796/1/AliElEnshasy2023_PrimerConcentrationandPreDenaturationTimeEffect.pdf |
| _version_ | 1817854179334422528 |
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| author | M., Azzahra M., Nurjayadi N. A., Pramudiyasih R. N., Kusumawati I., Maulana J. L., Declan G. I., Putri D. A., Juliansyah I., Krisdawati F., Kurniadewi I. R., Kartika D., Sukmawati L., Nastassya V., Saamia D. A., Oktaviani S. M., Wiranatha H., Ali El-Enshasy B., Abomoelak |
| author_facet | M., Azzahra M., Nurjayadi N. A., Pramudiyasih R. N., Kusumawati I., Maulana J. L., Declan G. I., Putri D. A., Juliansyah I., Krisdawati F., Kurniadewi I. R., Kartika D., Sukmawati L., Nastassya V., Saamia D. A., Oktaviani S. M., Wiranatha H., Ali El-Enshasy B., Abomoelak |
| author_sort | M., Azzahra |
| collection | ePrints |
| description | Foodborne disease is a global threat that can affect all sections of society, both in developed or developing countries. Bacillus cereus is a Gram-positive bacteria that can cause food poisoning disease in humans. [2] Real-Time PCR detection method is one of the molecular marker methods that has been widely recognized as a fast, reliable, sensitive and specific detection tool for detecting pathogenic bacteria. In previous studies, the optimum condition and formulas applied for cyt-K 2 primer pairs have been obtained using Real-Time PCR. The purpose of this study is to find out the best conditions work of the primer pair cyt-K Bacillus cereus on detecting bacteria target using variations of pre-denaturation time and primer concentration with Real-Time PCR method. The annealing temperature used for PCR is at 60°C with sample concentration 50 ng/μL of B. cereus. Real-time PCR detection of variations in pre-denaturation time and primer concentration obtained the best conditions for primer pair cyt-K work at minute 4 with a primer concentration of 10 pmol and successfully amplifying the target by producing a Ct value of B. cereus at 13.04. Based on the results of the study, the primer pair cyt-K were reproducible in detecting the target gene and in the further step, this research can be continued to developed a prototype detection kit for foodborne pathogen bacteria using Real-Time PCR method. |
| first_indexed | 2024-12-08T06:54:01Z |
| format | Conference or Workshop Item |
| id | utm.eprints-107796 |
| institution | Universiti Teknologi Malaysia - ePrints |
| language | English |
| last_indexed | 2024-12-08T06:54:01Z |
| publishDate | 2023 |
| record_format | dspace |
| spelling | utm.eprints-1077962024-10-02T07:37:27Z http://eprints.utm.my/107796/ Primer concentration and pre-denaturation time effect on cyt-K bacillus cereus detection using real-time PCR method. M., Azzahra M., Nurjayadi N. A., Pramudiyasih R. N., Kusumawati I., Maulana J. L., Declan G. I., Putri D. A., Juliansyah I., Krisdawati F., Kurniadewi I. R., Kartika D., Sukmawati L., Nastassya V., Saamia D. A., Oktaviani S. M., Wiranatha H., Ali El-Enshasy B., Abomoelak TP Chemical technology Foodborne disease is a global threat that can affect all sections of society, both in developed or developing countries. Bacillus cereus is a Gram-positive bacteria that can cause food poisoning disease in humans. [2] Real-Time PCR detection method is one of the molecular marker methods that has been widely recognized as a fast, reliable, sensitive and specific detection tool for detecting pathogenic bacteria. In previous studies, the optimum condition and formulas applied for cyt-K 2 primer pairs have been obtained using Real-Time PCR. The purpose of this study is to find out the best conditions work of the primer pair cyt-K Bacillus cereus on detecting bacteria target using variations of pre-denaturation time and primer concentration with Real-Time PCR method. The annealing temperature used for PCR is at 60°C with sample concentration 50 ng/μL of B. cereus. Real-time PCR detection of variations in pre-denaturation time and primer concentration obtained the best conditions for primer pair cyt-K work at minute 4 with a primer concentration of 10 pmol and successfully amplifying the target by producing a Ct value of B. cereus at 13.04. Based on the results of the study, the primer pair cyt-K were reproducible in detecting the target gene and in the further step, this research can be continued to developed a prototype detection kit for foodborne pathogen bacteria using Real-Time PCR method. 2023-07-03 Conference or Workshop Item PeerReviewed application/pdf en http://eprints.utm.my/107796/1/AliElEnshasy2023_PrimerConcentrationandPreDenaturationTimeEffect.pdf M., Azzahra and M., Nurjayadi and N. A., Pramudiyasih and R. N., Kusumawati and I., Maulana and J. L., Declan and G. I., Putri and D. A., Juliansyah and I., Krisdawati and F., Kurniadewi and I. R., Kartika and D., Sukmawati and L., Nastassya and V., Saamia and D. A., Oktaviani S. and M., Wiranatha and H., Ali El-Enshasy and B., Abomoelak (2023) Primer concentration and pre-denaturation time effect on cyt-K bacillus cereus detection using real-time PCR method. In: 1st International Conference on Sciences, Mathematics, and Education, ICoSMEd 2022, 13 October 2022 - 14 October 2022, Gorontalo, Sulawesi, Indonesia. http://dx.doi.org/10.1051/e3sconf/202340004011 |
| spellingShingle | TP Chemical technology M., Azzahra M., Nurjayadi N. A., Pramudiyasih R. N., Kusumawati I., Maulana J. L., Declan G. I., Putri D. A., Juliansyah I., Krisdawati F., Kurniadewi I. R., Kartika D., Sukmawati L., Nastassya V., Saamia D. A., Oktaviani S. M., Wiranatha H., Ali El-Enshasy B., Abomoelak Primer concentration and pre-denaturation time effect on cyt-K bacillus cereus detection using real-time PCR method. |
| title | Primer concentration and pre-denaturation time effect on cyt-K bacillus cereus detection using real-time PCR method. |
| title_full | Primer concentration and pre-denaturation time effect on cyt-K bacillus cereus detection using real-time PCR method. |
| title_fullStr | Primer concentration and pre-denaturation time effect on cyt-K bacillus cereus detection using real-time PCR method. |
| title_full_unstemmed | Primer concentration and pre-denaturation time effect on cyt-K bacillus cereus detection using real-time PCR method. |
| title_short | Primer concentration and pre-denaturation time effect on cyt-K bacillus cereus detection using real-time PCR method. |
| title_sort | primer concentration and pre denaturation time effect on cyt k bacillus cereus detection using real time pcr method |
| topic | TP Chemical technology |
| url | http://eprints.utm.my/107796/1/AliElEnshasy2023_PrimerConcentrationandPreDenaturationTimeEffect.pdf |
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