Advancements in multiple-step on-line preconcentration techniques for enhanced sensitivity in capillary electrophoresis
Multiple-step on-line preconcentration, a combination of at least two stacking techniques has been developed to increase the sensitivity in capillary electrophoresis (CE) for analytes in various samples. It is usually conducted sequentially, or in some cases, synergistically, where different stackin...
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| Format: | Article |
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John Wiley & Sons Inc
2024
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| author | Abdul Keyon, Aemi Syazwani Ng, Nyuk Ting Breadmore, Michael Charles |
| author_facet | Abdul Keyon, Aemi Syazwani Ng, Nyuk Ting Breadmore, Michael Charles |
| author_sort | Abdul Keyon, Aemi Syazwani |
| collection | ePrints |
| description | Multiple-step on-line preconcentration, a combination of at least two stacking techniques has been developed to increase the sensitivity in capillary electrophoresis (CE) for analytes in various samples. It is usually conducted sequentially, or in some cases, synergistically, where different stacking modes occur simultaneously. Multiple-step techniques allow simultaneous preconcentration and separation of various kinds of analytes in different complex samples in a single CE run. This review aims to provide recent advances in multiple-step on-line preconcentration techniques in CE. We critically review technical papers published for the last 7 years up until July 2024, subsequently organized according to the combination of the main stacking techniques, that is, field amplification, large volume sample stacking, transient isotachophoresis, micelle to solvent or micelle to cyclodextrin stacking, and others. The procedures, fundamental mechanism, analytical figures of merits achieved, and their feasibility for complicated sample matrices are reviewed. |
| first_indexed | 2025-02-19T02:44:38Z |
| format | Article |
| id | utm.eprints-108929 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2025-02-19T02:44:38Z |
| publishDate | 2024 |
| publisher | John Wiley & Sons Inc |
| record_format | dspace |
| spelling | utm.eprints-1089292024-12-15T06:07:29Z http://eprints.utm.my/108929/ Advancements in multiple-step on-line preconcentration techniques for enhanced sensitivity in capillary electrophoresis Abdul Keyon, Aemi Syazwani Ng, Nyuk Ting Breadmore, Michael Charles QD Chemistry Multiple-step on-line preconcentration, a combination of at least two stacking techniques has been developed to increase the sensitivity in capillary electrophoresis (CE) for analytes in various samples. It is usually conducted sequentially, or in some cases, synergistically, where different stacking modes occur simultaneously. Multiple-step techniques allow simultaneous preconcentration and separation of various kinds of analytes in different complex samples in a single CE run. This review aims to provide recent advances in multiple-step on-line preconcentration techniques in CE. We critically review technical papers published for the last 7 years up until July 2024, subsequently organized according to the combination of the main stacking techniques, that is, field amplification, large volume sample stacking, transient isotachophoresis, micelle to solvent or micelle to cyclodextrin stacking, and others. The procedures, fundamental mechanism, analytical figures of merits achieved, and their feasibility for complicated sample matrices are reviewed. John Wiley & Sons Inc 2024-09 Article PeerReviewed Abdul Keyon, Aemi Syazwani and Ng, Nyuk Ting and Breadmore, Michael Charles (2024) Advancements in multiple-step on-line preconcentration techniques for enhanced sensitivity in capillary electrophoresis. Journal of Separation Science, 47 (18). NA. ISSN 1615-9306 http://dx.doi.org/10.1002/jssc.202400519 DOI:10.1002/jssc.202400519 |
| spellingShingle | QD Chemistry Abdul Keyon, Aemi Syazwani Ng, Nyuk Ting Breadmore, Michael Charles Advancements in multiple-step on-line preconcentration techniques for enhanced sensitivity in capillary electrophoresis |
| title | Advancements in multiple-step on-line preconcentration techniques for enhanced sensitivity in capillary electrophoresis |
| title_full | Advancements in multiple-step on-line preconcentration techniques for enhanced sensitivity in capillary electrophoresis |
| title_fullStr | Advancements in multiple-step on-line preconcentration techniques for enhanced sensitivity in capillary electrophoresis |
| title_full_unstemmed | Advancements in multiple-step on-line preconcentration techniques for enhanced sensitivity in capillary electrophoresis |
| title_short | Advancements in multiple-step on-line preconcentration techniques for enhanced sensitivity in capillary electrophoresis |
| title_sort | advancements in multiple step on line preconcentration techniques for enhanced sensitivity in capillary electrophoresis |
| topic | QD Chemistry |
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