Bromelain-loaded silver nanoparticles: Formulation, characterization and biological activity
Bromelain (BL), a type of proteolytic enzymes from Ananas comosus (pineapple), has a variety of therapeutic potentials; nevertheless, its low bioavailability has restricted the clinical applications. The main aim of the current research was to develop a green synthesized Ag-BL nanoparticles via a co...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
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Elsevier B.V.
2024
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| author | Gheisari, Farshid Kasaee, Seyed Reza Mohamadian, Pardis Chelliapan, Shreeshivadasan Gholizadeh, Razieh Zareshahrabadi, Zahra Solhjoo, Seyyed Pooria Vafa, Ehsan Mosleh-Shirazi, Sareh Amani, Ali Mohammad Kamyab, Hesam |
| author_facet | Gheisari, Farshid Kasaee, Seyed Reza Mohamadian, Pardis Chelliapan, Shreeshivadasan Gholizadeh, Razieh Zareshahrabadi, Zahra Solhjoo, Seyyed Pooria Vafa, Ehsan Mosleh-Shirazi, Sareh Amani, Ali Mohammad Kamyab, Hesam |
| author_sort | Gheisari, Farshid |
| collection | ePrints |
| description | Bromelain (BL), a type of proteolytic enzymes from Ananas comosus (pineapple), has a variety of therapeutic potentials; nevertheless, its low bioavailability has restricted the clinical applications. The main aim of the current research was to develop a green synthesized Ag-BL nanoparticles via a cost-effective route to improve the physicochemical and antimicrobial characteristics as a novel agent for medical applications. In the present study, Bromelain was loaded on the silver nanoparticle surface via covalent bonds through green synthesis method. The physicochemical properties of Ag-BL nanoparticles characterized by following a detailed analysis over scanning electron microscopy, zeta potential, ultraviolet–visible absorption spectroscopy, transmission electron microscopy, fourier transform infrared spectroscopy, and X-ray diffraction studies. The antifungal and antibacterial activity were investigated by the Clinical and Laboratory Standards Institute methods and the results were compared with Ag NPs and plain bromelain. The cytotoxicity of Ag-BL nanoparticles was investigated by MTT assay. The TEM and XRD techniques revealed the successful biosynthesis of Ag-BL nanoparticles in spherical shape with the mean size of 7 to 24 nm and FTIR analysis pattern proved the attachments of bromelain and Ag nanoparticles in the fabricated nanostructure. The negative zeta value of the Ag-BL nanoparticles (−50 to –32 mV) indicates their high stability in the suspension. The Ag-BL nanoparticles demonstrated significant antimicrobial activity against various types of fungi and bacteria strains with a MIC range of 4–32 and 4–64 μg/mL, respectively. The MTT assay analysis determined the acceptable cell safety of Ag-BL nanoparticles. Generally, the results confirmed that Ag-BL nanoparticles could develop a new insight to produce antimicrobial products for biomedical applications. |
| first_indexed | 2025-02-19T02:44:54Z |
| format | Article |
| id | utm.eprints-109016 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2025-02-19T02:44:54Z |
| publishDate | 2024 |
| publisher | Elsevier B.V. |
| record_format | dspace |
| spelling | utm.eprints-1090162025-01-27T04:28:52Z http://eprints.utm.my/109016/ Bromelain-loaded silver nanoparticles: Formulation, characterization and biological activity Gheisari, Farshid Kasaee, Seyed Reza Mohamadian, Pardis Chelliapan, Shreeshivadasan Gholizadeh, Razieh Zareshahrabadi, Zahra Solhjoo, Seyyed Pooria Vafa, Ehsan Mosleh-Shirazi, Sareh Amani, Ali Mohammad Kamyab, Hesam Q Science (General) Bromelain (BL), a type of proteolytic enzymes from Ananas comosus (pineapple), has a variety of therapeutic potentials; nevertheless, its low bioavailability has restricted the clinical applications. The main aim of the current research was to develop a green synthesized Ag-BL nanoparticles via a cost-effective route to improve the physicochemical and antimicrobial characteristics as a novel agent for medical applications. In the present study, Bromelain was loaded on the silver nanoparticle surface via covalent bonds through green synthesis method. The physicochemical properties of Ag-BL nanoparticles characterized by following a detailed analysis over scanning electron microscopy, zeta potential, ultraviolet–visible absorption spectroscopy, transmission electron microscopy, fourier transform infrared spectroscopy, and X-ray diffraction studies. The antifungal and antibacterial activity were investigated by the Clinical and Laboratory Standards Institute methods and the results were compared with Ag NPs and plain bromelain. The cytotoxicity of Ag-BL nanoparticles was investigated by MTT assay. The TEM and XRD techniques revealed the successful biosynthesis of Ag-BL nanoparticles in spherical shape with the mean size of 7 to 24 nm and FTIR analysis pattern proved the attachments of bromelain and Ag nanoparticles in the fabricated nanostructure. The negative zeta value of the Ag-BL nanoparticles (−50 to –32 mV) indicates their high stability in the suspension. The Ag-BL nanoparticles demonstrated significant antimicrobial activity against various types of fungi and bacteria strains with a MIC range of 4–32 and 4–64 μg/mL, respectively. The MTT assay analysis determined the acceptable cell safety of Ag-BL nanoparticles. Generally, the results confirmed that Ag-BL nanoparticles could develop a new insight to produce antimicrobial products for biomedical applications. Elsevier B.V. 2024-03 Article PeerReviewed Gheisari, Farshid and Kasaee, Seyed Reza and Mohamadian, Pardis and Chelliapan, Shreeshivadasan and Gholizadeh, Razieh and Zareshahrabadi, Zahra and Solhjoo, Seyyed Pooria and Vafa, Ehsan and Mosleh-Shirazi, Sareh and Amani, Ali Mohammad and Kamyab, Hesam (2024) Bromelain-loaded silver nanoparticles: Formulation, characterization and biological activity. Inorganic Chemistry Communications, 161 (NA). NA-NA. ISSN 1387-7003 http://dx.doi.org/10.1016/j.inoche.2023.112006 DOI:10.1016/j.inoche.2023.112006 |
| spellingShingle | Q Science (General) Gheisari, Farshid Kasaee, Seyed Reza Mohamadian, Pardis Chelliapan, Shreeshivadasan Gholizadeh, Razieh Zareshahrabadi, Zahra Solhjoo, Seyyed Pooria Vafa, Ehsan Mosleh-Shirazi, Sareh Amani, Ali Mohammad Kamyab, Hesam Bromelain-loaded silver nanoparticles: Formulation, characterization and biological activity |
| title | Bromelain-loaded silver nanoparticles: Formulation, characterization and biological activity |
| title_full | Bromelain-loaded silver nanoparticles: Formulation, characterization and biological activity |
| title_fullStr | Bromelain-loaded silver nanoparticles: Formulation, characterization and biological activity |
| title_full_unstemmed | Bromelain-loaded silver nanoparticles: Formulation, characterization and biological activity |
| title_short | Bromelain-loaded silver nanoparticles: Formulation, characterization and biological activity |
| title_sort | bromelain loaded silver nanoparticles formulation characterization and biological activity |
| topic | Q Science (General) |
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