Protein interaction studies of crosslinked endolevanase aggregates from Bacillus lehensis G1
The efficiency of cross-linked enzyme aggregates (CLEAs) is mainly affected by the strength and binding site of the formed linkages between the enzyme and cross-linker. Therefore, this study investigated the impact of different macromolecular cross-linkers on various functional groups, their binding...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Penerbit UTM Press
2024
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| Online Access: | http://eprints.utm.my/109073/1/RosliMdIllias2024_ProteinInteractionStudiesofCrosslinked.pdf |
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| author | Makki, Hotaf Hassan Jaafar, Nardiah Rizwana A. Rahman, Roshanida Rahmat, Zaidah Ni Nyoman Tri Puspaningsih, Ni Nyoman Tri Puspaningsih Md. Illias, Rosli |
| author_facet | Makki, Hotaf Hassan Jaafar, Nardiah Rizwana A. Rahman, Roshanida Rahmat, Zaidah Ni Nyoman Tri Puspaningsih, Ni Nyoman Tri Puspaningsih Md. Illias, Rosli |
| author_sort | Makki, Hotaf Hassan |
| collection | ePrints |
| description | The efficiency of cross-linked enzyme aggregates (CLEAs) is mainly affected by the strength and binding site of the formed linkages between the enzyme and cross-linker. Therefore, this study investigated the impact of different macromolecular cross-linkers on various functional groups, their binding energy, and intermolecular interaction in generating CLEAs of endolevanase from Bacillus lehensis G1 (rlevblg1), through the combination of computational and experimental analysis. Due to the distanced bonding of dextran from the active site, rlevblg1 cross-linked with dextran (rlevblg1-dexCLEAs) exhibited the highest binding affinity (− 7.1 kcal/mol) and activity recovery compared to six other cross-linkers. Thus, the role of computational cross-linker screening is confirmed as a crucial step to predict strong attachment and construct efficient CLEAs. |
| first_indexed | 2025-02-19T02:45:04Z |
| format | Article |
| id | utm.eprints-109073 |
| institution | Universiti Teknologi Malaysia - ePrints |
| language | English |
| last_indexed | 2025-02-19T02:45:04Z |
| publishDate | 2024 |
| publisher | Penerbit UTM Press |
| record_format | dspace |
| spelling | utm.eprints-1090732025-01-28T06:53:30Z http://eprints.utm.my/109073/ Protein interaction studies of crosslinked endolevanase aggregates from Bacillus lehensis G1 Makki, Hotaf Hassan Jaafar, Nardiah Rizwana A. Rahman, Roshanida Rahmat, Zaidah Ni Nyoman Tri Puspaningsih, Ni Nyoman Tri Puspaningsih Md. Illias, Rosli Q Science (General) The efficiency of cross-linked enzyme aggregates (CLEAs) is mainly affected by the strength and binding site of the formed linkages between the enzyme and cross-linker. Therefore, this study investigated the impact of different macromolecular cross-linkers on various functional groups, their binding energy, and intermolecular interaction in generating CLEAs of endolevanase from Bacillus lehensis G1 (rlevblg1), through the combination of computational and experimental analysis. Due to the distanced bonding of dextran from the active site, rlevblg1 cross-linked with dextran (rlevblg1-dexCLEAs) exhibited the highest binding affinity (− 7.1 kcal/mol) and activity recovery compared to six other cross-linkers. Thus, the role of computational cross-linker screening is confirmed as a crucial step to predict strong attachment and construct efficient CLEAs. Penerbit UTM Press 2024-03 Article PeerReviewed application/pdf en http://eprints.utm.my/109073/1/RosliMdIllias2024_ProteinInteractionStudiesofCrosslinked.pdf Makki, Hotaf Hassan and Jaafar, Nardiah Rizwana and A. Rahman, Roshanida and Rahmat, Zaidah and Ni Nyoman Tri Puspaningsih, Ni Nyoman Tri Puspaningsih and Md. Illias, Rosli (2024) Protein interaction studies of crosslinked endolevanase aggregates from Bacillus lehensis G1. Jurnal Teknologi, 86 (2). pp. 159-167. ISSN 0127-9696 http://dx.doi.org/10.11113/jurnalteknologi.v86.20888 DOI:10.11113/jurnalteknologi.v86.20888 |
| spellingShingle | Q Science (General) Makki, Hotaf Hassan Jaafar, Nardiah Rizwana A. Rahman, Roshanida Rahmat, Zaidah Ni Nyoman Tri Puspaningsih, Ni Nyoman Tri Puspaningsih Md. Illias, Rosli Protein interaction studies of crosslinked endolevanase aggregates from Bacillus lehensis G1 |
| title | Protein interaction studies of crosslinked endolevanase aggregates from Bacillus lehensis G1 |
| title_full | Protein interaction studies of crosslinked endolevanase aggregates from Bacillus lehensis G1 |
| title_fullStr | Protein interaction studies of crosslinked endolevanase aggregates from Bacillus lehensis G1 |
| title_full_unstemmed | Protein interaction studies of crosslinked endolevanase aggregates from Bacillus lehensis G1 |
| title_short | Protein interaction studies of crosslinked endolevanase aggregates from Bacillus lehensis G1 |
| title_sort | protein interaction studies of crosslinked endolevanase aggregates from bacillus lehensis g1 |
| topic | Q Science (General) |
| url | http://eprints.utm.my/109073/1/RosliMdIllias2024_ProteinInteractionStudiesofCrosslinked.pdf |
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