Cloning of influenza B NS1 gene in Escherichia Coli
The non-structural NS1 protein of influenza B virus is a multifunctional virulence protein which is involved in the transport of viral RNA. Inside the host cell, NS1B antagonizes and inhibits the a/ß interferon system which is induced as host antiviral response. Moreover, it prevents the activation...
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| Format: | Thesis |
| Language: | English |
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2010
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| Online Access: | http://eprints.utm.my/12283/4/AliAdelDawoodMFBB2010.pdf |
| _version_ | 1796855049016573952 |
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| author | Adel Dawood, Ali |
| author_facet | Adel Dawood, Ali |
| author_sort | Adel Dawood, Ali |
| collection | ePrints |
| description | The non-structural NS1 protein of influenza B virus is a multifunctional virulence protein which is involved in the transport of viral RNA. Inside the host cell, NS1B antagonizes and inhibits the a/ß interferon system which is induced as host antiviral response. Moreover, it prevents the activation of double-stranded-RNA activated protein kinase (PKR) by binding to dsRNA and inhibits the maturation of GAS8 (gene of tumor suppressor). NS1 protein is utilized as a target for diagnostic of influenza viruses in infected animals. pUC57 carrying NS1 synthetic gene of influenza B was attempted to be transformed into Escherichia coli strain BL21(DE3). NS1B was extracted and attempted to be cloned into prokaryotic expression vectors pET-32b, pET-32a, pQE-81L and pQE-80L, respectively using restriction digestion enzymes (SacI, PstI and HindIII). Then, recombinant DNA was attempted to be transformed into Escherichia coli strains BL21(DE3) and DH5a. |
| first_indexed | 2024-03-05T18:22:55Z |
| format | Thesis |
| id | utm.eprints-12283 |
| institution | Universiti Teknologi Malaysia - ePrints |
| language | English |
| last_indexed | 2024-03-05T18:22:55Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | utm.eprints-122832017-09-20T04:08:49Z http://eprints.utm.my/12283/ Cloning of influenza B NS1 gene in Escherichia Coli Adel Dawood, Ali RZ Other systems of medicine The non-structural NS1 protein of influenza B virus is a multifunctional virulence protein which is involved in the transport of viral RNA. Inside the host cell, NS1B antagonizes and inhibits the a/ß interferon system which is induced as host antiviral response. Moreover, it prevents the activation of double-stranded-RNA activated protein kinase (PKR) by binding to dsRNA and inhibits the maturation of GAS8 (gene of tumor suppressor). NS1 protein is utilized as a target for diagnostic of influenza viruses in infected animals. pUC57 carrying NS1 synthetic gene of influenza B was attempted to be transformed into Escherichia coli strain BL21(DE3). NS1B was extracted and attempted to be cloned into prokaryotic expression vectors pET-32b, pET-32a, pQE-81L and pQE-80L, respectively using restriction digestion enzymes (SacI, PstI and HindIII). Then, recombinant DNA was attempted to be transformed into Escherichia coli strains BL21(DE3) and DH5a. 2010-11 Thesis NonPeerReviewed application/pdf en http://eprints.utm.my/12283/4/AliAdelDawoodMFBB2010.pdf Adel Dawood, Ali (2010) Cloning of influenza B NS1 gene in Escherichia Coli. Masters thesis, Universiti Teknologi Malaysia, Faculty of Biosciences and Bioengineering. |
| spellingShingle | RZ Other systems of medicine Adel Dawood, Ali Cloning of influenza B NS1 gene in Escherichia Coli |
| title | Cloning of influenza B NS1 gene in Escherichia Coli |
| title_full | Cloning of influenza B NS1 gene in Escherichia Coli |
| title_fullStr | Cloning of influenza B NS1 gene in Escherichia Coli |
| title_full_unstemmed | Cloning of influenza B NS1 gene in Escherichia Coli |
| title_short | Cloning of influenza B NS1 gene in Escherichia Coli |
| title_sort | cloning of influenza b ns1 gene in escherichia coli |
| topic | RZ Other systems of medicine |
| url | http://eprints.utm.my/12283/4/AliAdelDawoodMFBB2010.pdf |
| work_keys_str_mv | AT adeldawoodali cloningofinfluenzabns1geneinescherichiacoli |