Cloning, extracellular expression and characterization of a predominant beta-CGTase from Bacillus sp G1 in E-coli

Cloning, extracellular expression and characterization of a predominant beta-CGTase from Bacillus sp G1 in E-coli

The cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) gene from Bacillus sp. G1 was successfully isolated and cloned into Escherichia coli. Analysis of the nucleotide sequence revealed the presence of an open reading frame of 2,109 bp and encoded a 674 amino acid protein. Purified CGTase exhibit...

Full description

Bibliographic Details
Main Authors:	Ong, Rui Min, Goh, Kian Mau, Mahadi, Nor Muhammad, Hassan, Osman, Raja Abdul Rahman, Raja Noor Zaliha, Md. Illias, Rosli
Format:	Article
Published:	Springer Verlag 2008
Subjects:	Q Science (General)

Similar Items

Cloning, extracellular expression and characterization of predominant β-CGTase from Bacillus sp. G1 in E. coli
by: Ong, Rui Min, et al.
Published: (2008)

Cloning, extracellular expression and characterization of a predominant Î²-CGTase from Bacillus sp. G1 in E. coli
by: Ong, Rui Min, et al.
Published: (2008)

Molecular modeling of a predominant β-CGTase G1 and analysis of ionic interaction in CGTase
by: Goh, Kian Mau, et al.
Published: (2008)

Molecular modeling of a predominant b-CGTase G1 and analysis of ionic interaction in CGTase
by: Kian Mau Goh, author, et al.
Published: (2009)

A predominant Î²-CGTase G1 engineered to elucidate the relationship between protein structure and product specificity
by: Goh, Kin Mau, et al.
Published: (2009)

Purification and characterization of recombinant enzyme CGTase G1
by: Goh, Kian Mau, et al.
Published: (2005)

A predominant β-CGTase G1 engineered to elucidate the relationship between protein structure and product specificity
by: Goh, Kian Mau, et al.
Published: (2009)

Improvement of cyclodextrin glucanotransferase G1 (CGTase) product specificity by protein engineering and reaction parameter manipulation
by: Md. Illias, Rosli, et al.
Published: (2009)

The effects of reaction conditions on the production of Î³-cyclodextrin from tapioca starch by using a novel recombinant engineered CGTase
by: Goh, Kian Mau, et al.
Published: (2007)

Secretory overexpression of CGTase gene from bacillus sp. G1 in escherichia coli
by: Lo, Po Kim, et al.
Published: (2005)

Isolation,cloning, sequencing and expression of cyclomaltodextrin glucanotransferase (CGTase) gene from bacillus sp /
by: Rosli Md. Illias
Published: (2005)

From gene to process : CGtase as a role model
by: Md. Illias, , Rosli, et al.
Published: (2008)

Molecular cloning of CGTase gene from Bacillus sp G1 /
by: Ong, Rui Min, et al.
Published: (2002)

Cloning and sequencing of CGTase gene from bacillus sp TS1 /
by: 429762 Kamalesh Rahman, et al.
Published: (2004)

Cloning and sequencing of CGTase gene from Bacillus sp G1 /
by: Ong, Rui Min, et al.
Published: (2003)

Secretory overexpression of CGTase gene from Bacillus sp. G1 in Escherichia coli /
by: Lo, Po Kim, et al.
Published: (2005)

Domain replacement to elucidate the role of B domain in cgtase thermostability and activity
by: Poh, Hong Goh, et al.
Published: (2012)

Excretory over-expression of Bacillus sp. G1 cyclodextrin glucanotransferase (CGTase) in Escherichia coli: Optimization of the cultivation conditions by response surface methodology
by: Lo, Po Kim, et al.
Published: (2007)

Improvement of cyclodextrin glucanotransferase GI (CGTase) product specificity by protein engineering and reaction parameter manipulation /
by: Rosli Md. Illias, author, et al.
Published: (2009)

Pruification and characterization of recombinant enzy me CGTaseG1 /
by: Goh Kian Maui, author, et al.
Published: (2005)

Use of megaprimer and overlapping extension pcr (OE-PCR) to mutagenize and enhance cyclodextrin glucosyltransferase (CGTASE) function
by: Goh, Kian Mau, et al.
Published: (2017)

Cloning and characterization of two new thermostable and alkalitolerant α-amylase from the Anoxybacillus species that produce high levels of maltose
by: Chai, Yen Yen, et al.
Published: (2012)

Improvement of excretory overexpression for Bacillus sp. G1 cyclodextrin glucanotransferase (CGTase) in recombinant Escherichia coli through medium optimization
by: Lo, P. K., et al.
Published: (2009)

Production mutant CGtase enzyme in E.coli /
by: Siti Atikah Mohamad, author, et al.
Published: (2008)

A comparison of conventional and miniprimer PCR to elucidate bacteria diversity in Malaysia Ulu Slim hot spring using 16S rDNA clone library
by: Goh, Kian Mau, et al.
Published: (2011)

A comparison of conventional and miniprimer PCR to elucidate bacteria diversity in Malaysia Ulu Slim hot spring using 16S rDNA clone library
by: Goh, Kian Mau, et al.
Published: (2011)

Production of cyclodextrin glucanotransferase (CGTase) from Bacillus sp. TS1-1: process optimisation
by: Ahmad Tarmizi, Azmil Haizam, et al.
Published: (2003)

Isolation of Alkalophilic Bacillus SP. G1 and the production of Cyclodextrin Glucanotransferase (CGTase) /
by: 193412 Tien, Siew Fen, et al.
Published: (2001)

Cyclodextrin Glucanotransferase (CGTase) production from local isolated bacillus sp. ts1. /
by: Azmil Haizam Ahmad Tarmizi, et al.
Published: (2002)

Optimization of medium formulation for the production of cyclodextrin glucanotransferase (CGTase) from bacillus stearothermophilus HRI /
by: Roshanida Abd. Rahman, et al.
Published: (2002)

Production of cyclodextrin glucanotransferase (CGTase) from bacillus sp. TS1-1 : process optimisation /
by: 202977 Azmi Haizam Tarmizi, et al.
Published: (2003)

Production of Cyclodextrin Glucanotransferase (CGTase) from Bacillus sp. TS1-1 : by fed-batch fermentation /
by: Roshanida Abd. Rahman, et al.
Published: (2003)

Fed-batch fermentations of recombinant escherichia coli to produce bacillus macerans CGTase /
by: Park, Yong-Cheol

The effects of reaction conditions on the production of gamma-cyclodextrin from tapioca starch by using a novel recombinant engineered CGTase
by: Goh, K. M., et al.
Published: (2007)

Production of cyclodextrin glucanotransferase (CGTase) from Bacillus stearothermophilus HR1 in shake flasks /
by: Rosli Md. Illias, et al.
Published: (2003)

Initial screening of fermentation variables for the production of novel Cyclomaltodextrin glucanotransferase (CGTase) from local isolated Bacillus stearothermophilus HR1
by: Wong, T. E., et al.
Published: (2005)

Effects of the Immobilization of Recombinant Escherichia Coli on Cyclodextrin Glucanotransferase (CGTase) Excretion and Cell Viability
by: Rohaida, Che Man, et al.
Published: (2015)

Effects of the immobilization of recombinant Escherichia coli on cyclodextrin glucanotransferase (cgtase) excretion and cell viability
by: Mohd Fuzi, Siti Fatimah Zaharah, et al.
Published: (2015)

Effects of the immobilization of recombinant Escherichia coli on cyclodextrin glucanotransferase (CGTase) excretion and cell viability
by: Che Man, Rohaida, et al.
Published: (2015)

Production of cyclodextrin glucanotransferase (CGTase) from Bacillus stearothermophilus HR1 in shake flasks
by: Wong, Tien Ee, et al.
Published: (2003)