| Summary: | The anti inflammatory activity of the leaf extract of Vitex negundo was investigated on topical administration using the TPA. (tetradecanoylphorbol acetate)-induced mouse ear inflammation model. The crude methanolic extract of V. negundo showed moderate inhibition (74%) at a dose of 2 mg per ear. The methanol extract was then subjected to liquid-liquid fractionation with petroleum ether, chloroform, ethyl-acetate and ethanol. Bioassay-guided chromatographic fractionation of these fractions led to the isolation of 3,4-dihydroxybenzoic acid as the bioactive principle responsible for the anti-inflammatory activity. The structures of the pure compounds were elucidated using various spectroscopic techniques.
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