A Potential Use Of Dehalogenase D (Dehd) From Rhizobium Sp. For Industrial Process
The Rhizobium sp. DehL and DehD were produced by heterologous expression of the cloned gene in E.coli and both proteins purified using anion-exchange column chromatography. DehL and DehD were characterised by kinetic analysis to determine their Km, Kcat and the Specificity constant values. The kinet...
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| Format: | Article |
| Language: | English |
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Penerbit UTM Press
2003
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| Online Access: | http://eprints.utm.my/1467/1/JT38C%5B7%5D.pdf |
| _version_ | 1825909076385595392 |
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| author | Huyop, Fahrul Z. Ronald, A. Cooper |
| author_facet | Huyop, Fahrul Z. Ronald, A. Cooper |
| author_sort | Huyop, Fahrul Z. |
| collection | ePrints |
| description | The Rhizobium sp. DehL and DehD were produced by heterologous expression of the cloned gene in E.coli and both proteins purified using anion-exchange column chromatography. DehL and DehD were characterised by kinetic analysis to determine their Km, Kcat and the Specificity constant values. The kinetic analysis results showed that DehD from Rhizobium sp. has lower Km value (0.04 mM with D,L-2-CP) and higher Kcat (6.28 sec–1 for D,L-2-CP) and Specificity constants (1.46 × 105 M–1sec–1 for D,L-2-CP) compared to other D-specific dehalogenases from different organism suggesting DehD enzyme from Rhizobium sp. is better catalysts. D-2-haloacid dehalogenase is important for industrial biocatalysis compared to the L-2-haloacid and the kinetic data of DehD hold promise for further development to be used in an industrial process. |
| first_indexed | 2024-03-05T17:56:45Z |
| format | Article |
| id | utm.eprints-1467 |
| institution | Universiti Teknologi Malaysia - ePrints |
| language | English |
| last_indexed | 2024-03-05T17:56:45Z |
| publishDate | 2003 |
| publisher | Penerbit UTM Press |
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| spelling | utm.eprints-14672017-11-01T04:17:41Z http://eprints.utm.my/1467/ A Potential Use Of Dehalogenase D (Dehd) From Rhizobium Sp. For Industrial Process Huyop, Fahrul Z. Ronald, A. Cooper QH301 Biology The Rhizobium sp. DehL and DehD were produced by heterologous expression of the cloned gene in E.coli and both proteins purified using anion-exchange column chromatography. DehL and DehD were characterised by kinetic analysis to determine their Km, Kcat and the Specificity constant values. The kinetic analysis results showed that DehD from Rhizobium sp. has lower Km value (0.04 mM with D,L-2-CP) and higher Kcat (6.28 sec–1 for D,L-2-CP) and Specificity constants (1.46 × 105 M–1sec–1 for D,L-2-CP) compared to other D-specific dehalogenases from different organism suggesting DehD enzyme from Rhizobium sp. is better catalysts. D-2-haloacid dehalogenase is important for industrial biocatalysis compared to the L-2-haloacid and the kinetic data of DehD hold promise for further development to be used in an industrial process. Penerbit UTM Press 2003-06 Article PeerReviewed application/pdf en http://eprints.utm.my/1467/1/JT38C%5B7%5D.pdf Huyop, Fahrul Z. and Ronald, A. Cooper (2003) A Potential Use Of Dehalogenase D (Dehd) From Rhizobium Sp. For Industrial Process. Jurnal Teknologi C (38C). pp. 69-75. ISSN 0127-9696 |
| spellingShingle | QH301 Biology Huyop, Fahrul Z. Ronald, A. Cooper A Potential Use Of Dehalogenase D (Dehd) From Rhizobium Sp. For Industrial Process |
| title | A Potential Use Of Dehalogenase D (Dehd) From Rhizobium Sp. For Industrial Process |
| title_full | A Potential Use Of Dehalogenase D (Dehd) From Rhizobium Sp. For Industrial Process |
| title_fullStr | A Potential Use Of Dehalogenase D (Dehd) From Rhizobium Sp. For Industrial Process |
| title_full_unstemmed | A Potential Use Of Dehalogenase D (Dehd) From Rhizobium Sp. For Industrial Process |
| title_short | A Potential Use Of Dehalogenase D (Dehd) From Rhizobium Sp. For Industrial Process |
| title_sort | potential use of dehalogenase d dehd from rhizobium sp for industrial process |
| topic | QH301 Biology |
| url | http://eprints.utm.my/1467/1/JT38C%5B7%5D.pdf |
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