Palm kernel cake as substrate for ß-Mannanase production by Bacillus subtilis ATCC3366 under submerged and solid state fermentations
Beta-Mannanase activity and growth profile of Bacillus subtilis ATCC3366 during submerged fermentation (SmF) and solid state fermentation (SSF) using palm kernel cake as substrate were examined in this study. After 36 h of fermentation, ß-Mannanase produced by Bacillus subtilis in SmF system was re...
| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English |
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2006
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| Online Access: | http://eprints.utm.my/189/1/ChinHooiChuan2006_Palm_kernel_cake_as_substrate.pdf |
| _version_ | 1796853043718782976 |
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| author | Chuan, Chin Hooi Krishnaiah, Kamatam Michael Wong, Clemente Janaun, Jidon |
| author_facet | Chuan, Chin Hooi Krishnaiah, Kamatam Michael Wong, Clemente Janaun, Jidon |
| author_sort | Chuan, Chin Hooi |
| collection | ePrints |
| description | Beta-Mannanase activity and growth profile of Bacillus subtilis ATCC3366 during submerged fermentation (SmF) and solid state fermentation (SSF) using palm kernel cake as substrate were examined in this study. After 36 h of fermentation, ß-Mannanase produced by Bacillus subtilis in SmF system was recorded at 8 U/ml, whereas in SSF system was 230 U/g dry PKC. In SmF system, a long lag phase of 28 h were observed before the number of viable cells reached the maximum of 2.08 × 108 CFU/ml at 36 h; whereas in SSF system was 3.75 × 109 CFU/ml at 24 h, with no lag phase observed. Optimum conditions of enzyme activity were also examined. The optimum pH was 7.0, and optimum temperature was 55oC. |
| first_indexed | 2024-03-05T17:53:46Z |
| format | Conference or Workshop Item |
| id | utm.eprints-189 |
| institution | Universiti Teknologi Malaysia - ePrints |
| language | English |
| last_indexed | 2024-03-05T17:53:46Z |
| publishDate | 2006 |
| record_format | dspace |
| spelling | utm.eprints-1892017-08-30T01:36:46Z http://eprints.utm.my/189/ Palm kernel cake as substrate for ß-Mannanase production by Bacillus subtilis ATCC3366 under submerged and solid state fermentations Chuan, Chin Hooi Krishnaiah, Kamatam Michael Wong, Clemente Janaun, Jidon TP Chemical technology Beta-Mannanase activity and growth profile of Bacillus subtilis ATCC3366 during submerged fermentation (SmF) and solid state fermentation (SSF) using palm kernel cake as substrate were examined in this study. After 36 h of fermentation, ß-Mannanase produced by Bacillus subtilis in SmF system was recorded at 8 U/ml, whereas in SSF system was 230 U/g dry PKC. In SmF system, a long lag phase of 28 h were observed before the number of viable cells reached the maximum of 2.08 × 108 CFU/ml at 36 h; whereas in SSF system was 3.75 × 109 CFU/ml at 24 h, with no lag phase observed. Optimum conditions of enzyme activity were also examined. The optimum pH was 7.0, and optimum temperature was 55oC. 2006 Conference or Workshop Item PeerReviewed application/pdf en http://eprints.utm.my/189/1/ChinHooiChuan2006_Palm_kernel_cake_as_substrate.pdf Chuan, Chin Hooi and Krishnaiah, Kamatam and Michael Wong, Clemente and Janaun, Jidon (2006) Palm kernel cake as substrate for ß-Mannanase production by Bacillus subtilis ATCC3366 under submerged and solid state fermentations. In: 1st International Conference on Natural Resources Engineering & Technology 2006, 24-25th July 2006, Putrajaya, Malaysia. |
| spellingShingle | TP Chemical technology Chuan, Chin Hooi Krishnaiah, Kamatam Michael Wong, Clemente Janaun, Jidon Palm kernel cake as substrate for ß-Mannanase production by Bacillus subtilis ATCC3366 under submerged and solid state fermentations |
| title | Palm kernel cake as substrate for ß-Mannanase production by Bacillus subtilis ATCC3366 under submerged and solid state fermentations |
| title_full | Palm kernel cake as substrate for ß-Mannanase production by Bacillus subtilis ATCC3366 under submerged and solid state fermentations |
| title_fullStr | Palm kernel cake as substrate for ß-Mannanase production by Bacillus subtilis ATCC3366 under submerged and solid state fermentations |
| title_full_unstemmed | Palm kernel cake as substrate for ß-Mannanase production by Bacillus subtilis ATCC3366 under submerged and solid state fermentations |
| title_short | Palm kernel cake as substrate for ß-Mannanase production by Bacillus subtilis ATCC3366 under submerged and solid state fermentations |
| title_sort | palm kernel cake as substrate for ay mannanase production by bacillus subtilis atcc3366 under submerged and solid state fermentations |
| topic | TP Chemical technology |
| url | http://eprints.utm.my/189/1/ChinHooiChuan2006_Palm_kernel_cake_as_substrate.pdf |
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