Biodegradation of 4-aminobenzenesulfonate by Ralstonia sp. PBA and Hydrogenophaga sp. PBC isolated from textile wastewater treatment plant
A co-culture consisting of Hydrogenophaga sp. PBC and Ralstonia sp. PBA, isolated from textile wastewater treatment plant could tolerate up to 100mM 4-aminobenzenesulfonate (4-ABS) and utilize it as sole carbon, nitrogen and sulfur source under aerobic condition. The biodegradation of 4-ABS resulted...
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Elsevier Limited
2010
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| author | Gan, H. M. Shahir, Shafinaz Ibrahim, Zaharah Yahya, Adibah |
| author_facet | Gan, H. M. Shahir, Shafinaz Ibrahim, Zaharah Yahya, Adibah |
| author_sort | Gan, H. M. |
| collection | ePrints |
| description | A co-culture consisting of Hydrogenophaga sp. PBC and Ralstonia sp. PBA, isolated from textile wastewater treatment plant could tolerate up to 100mM 4-aminobenzenesulfonate (4-ABS) and utilize it as sole carbon, nitrogen and sulfur source under aerobic condition. The biodegradation of 4-ABS resulted in the release of nitrogen and sulfur in the form of ammonium and sulfate respectively. Ninety-eight percent removal of chemical oxygen demand attributed to 20mM of 4-ABS in cell-free supernatant could be achieved after 118h. Effective biodegradation of 4-ABS occurred at pH ranging from 6 to 8. During batch culture with 4-ABS as sole carbon and nitrogen source, the ratio of strain PBA to PBC was dynamic and a critical concentration of strain PBA has to be reached in order to enable effective biodegradation of 4-ABS. Haldane inhibition model was used to fit the degradation rate at different initial concentrations and the parameters µmax, Ks and Ki were determined to be 0.13h-1, 1.3mM and 42mM respectively. HPLC analyses revealed traced accumulation of 4-sulfocatechol and at least four unidentified metabolites during biodegradation. This is the first study to report on the characterization of 4-ABS-degrading bacterial consortium that was isolated from textile wastewater treatment plant. |
| first_indexed | 2024-03-05T18:36:28Z |
| format | Article |
| id | utm.eprints-22880 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T18:36:28Z |
| publishDate | 2010 |
| publisher | Elsevier Limited |
| record_format | dspace |
| spelling | utm.eprints-228802018-03-22T10:30:00Z http://eprints.utm.my/22880/ Biodegradation of 4-aminobenzenesulfonate by Ralstonia sp. PBA and Hydrogenophaga sp. PBC isolated from textile wastewater treatment plant Gan, H. M. Shahir, Shafinaz Ibrahim, Zaharah Yahya, Adibah Q Science (General) A co-culture consisting of Hydrogenophaga sp. PBC and Ralstonia sp. PBA, isolated from textile wastewater treatment plant could tolerate up to 100mM 4-aminobenzenesulfonate (4-ABS) and utilize it as sole carbon, nitrogen and sulfur source under aerobic condition. The biodegradation of 4-ABS resulted in the release of nitrogen and sulfur in the form of ammonium and sulfate respectively. Ninety-eight percent removal of chemical oxygen demand attributed to 20mM of 4-ABS in cell-free supernatant could be achieved after 118h. Effective biodegradation of 4-ABS occurred at pH ranging from 6 to 8. During batch culture with 4-ABS as sole carbon and nitrogen source, the ratio of strain PBA to PBC was dynamic and a critical concentration of strain PBA has to be reached in order to enable effective biodegradation of 4-ABS. Haldane inhibition model was used to fit the degradation rate at different initial concentrations and the parameters µmax, Ks and Ki were determined to be 0.13h-1, 1.3mM and 42mM respectively. HPLC analyses revealed traced accumulation of 4-sulfocatechol and at least four unidentified metabolites during biodegradation. This is the first study to report on the characterization of 4-ABS-degrading bacterial consortium that was isolated from textile wastewater treatment plant. Elsevier Limited 2010 Article PeerReviewed Gan, H. M. and Shahir, Shafinaz and Ibrahim, Zaharah and Yahya, Adibah (2010) Biodegradation of 4-aminobenzenesulfonate by Ralstonia sp. PBA and Hydrogenophaga sp. PBC isolated from textile wastewater treatment plant. Chemosphere, 82 (4). 507 - 513. ISSN 0045-6535 http://dx.doi.org/10.1016/j.chemosphere.2010.10.094 10.1016/j.chemosphere.2010.10.094 |
| spellingShingle | Q Science (General) Gan, H. M. Shahir, Shafinaz Ibrahim, Zaharah Yahya, Adibah Biodegradation of 4-aminobenzenesulfonate by Ralstonia sp. PBA and Hydrogenophaga sp. PBC isolated from textile wastewater treatment plant |
| title | Biodegradation of 4-aminobenzenesulfonate by Ralstonia sp. PBA and Hydrogenophaga sp. PBC isolated from textile wastewater treatment plant |
| title_full | Biodegradation of 4-aminobenzenesulfonate by Ralstonia sp. PBA and Hydrogenophaga sp. PBC isolated from textile wastewater treatment plant |
| title_fullStr | Biodegradation of 4-aminobenzenesulfonate by Ralstonia sp. PBA and Hydrogenophaga sp. PBC isolated from textile wastewater treatment plant |
| title_full_unstemmed | Biodegradation of 4-aminobenzenesulfonate by Ralstonia sp. PBA and Hydrogenophaga sp. PBC isolated from textile wastewater treatment plant |
| title_short | Biodegradation of 4-aminobenzenesulfonate by Ralstonia sp. PBA and Hydrogenophaga sp. PBC isolated from textile wastewater treatment plant |
| title_sort | biodegradation of 4 aminobenzenesulfonate by ralstonia sp pba and hydrogenophaga sp pbc isolated from textile wastewater treatment plant |
| topic | Q Science (General) |
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